Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100866
Name   oriT_pKPC-98f in_silico
Organism   Enterobacter cloacae complex sp. ECNIH7
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP017993 (2042..2216 [+], 175 nt)
oriT length   175 nt
IRs (inverted repeats)      2..11, 15..24  (GTGTCGGGGC..GCCCTGAACC)
Location of nic site      55..56
Conserved sequence flanking the
  nic site    		  CTGG|CTTA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 175 nt

>oriT_pKPC-98f
GGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTACTATGTTGGCACGGATGAGGGTGTCAGTGAAGTGCTTCATGTGGCAGGAGAAAAAAGGCGGCACCGGTGCGTCAGCAGAATATGTGATACAGGATATATTCCGCTTCCTCGCTCAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   938 GenBank   WP_047719615
Name   PutativerelaxaseofpKPC-98f insolico UniProt ID   _
Length   498 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 498 a.a.        Molecular weight: 55043.39 Da        Isoelectric Point: 9.0463

>WP_047719615.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacteriaceae]
MIVKFHPRGRGGGAGPVDYLLGKDRQREGATVLQGKPEEVRELIDASPYVKKYTSGVLSFAEADLPPGQR
EKLMASFERVLMPGLDKDQYSILWVEHADKGRLELNFLIPNTELLTGRRLQPYYDRADRPRIDAWQTIVN
GRLGLHDPNAPENRRALVTPSGLPKTKQEAAEAITRGLLTLASSGELKSRQDVTDTLEGAGFEVVRTTKS
SISIADPDGGRNIRLKGAIYEQSFNAGEGLRAAIESAAAEYRRDAESRIQRARAVCQSGTERKREENQRR
HPRPRAEYERGDGEKPAERTADCRADMAHHPDGVSAADRHEREHSVVAGAADNRQLHASSSAGRYPGEND
RPDVGSALSGEQRRPEVSYPAAGDTNRSHPLRRDDMDPPETGVAENDRAGKAVAERIRAATAGLLAKAGR
MGERLRGMAEHVWAYATGERGAERARVGLEHAGAEFKRAAAPLVRELNAIELRQRENEYQKTLELERQPQ
KTYRGPTL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(57-238)


  Protein structure



No available structure.




Host bacterium

ID   1326 GenBank   NZ_CP017993
Plasmid name   pKPC-98f Incompatibility group   Col440II
Plasmid size   21186 bp Coordinate of oriT [Strand]   2042..2216 [+]
Host baterium   Enterobacter cloacae complex sp. ECNIH7

Cargo genes

Drug resistance gene   blaKPC-2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -