Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100859 |
| Name | oriT_pENH91  |
| Organism | Cupriavidus necator strain NH9 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP017760 (30494..31084 [-], 591 nt) |
| oriT length | 591 nt |
| IRs (inverted repeats) | 148..160, 166..178 (AGGCGCTGCCGGT..ACCGGAAGCGCCT) 250..255, 260..265 (GCCGGC..GCCGGC) 318..336, 339..357 (GCGAAGATAGATAACCGGC..GCCGGTTAGCTAACTTCGC) |
| Location of nic site | 365..366 |
| Conserved sequence flanking the nic site |
CATCCTG|C |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 591 nt
>oriT_pENH91
TGTCGCTACCCCTTGAGCGCGTGCGCCGTGTGTCGCGCGTAGGTCGCCACGGCTCGCCAGTAGGCGGCCATCGGCCCCTTGCGGCGTGCCCATGCCTTTTCCGCCTCGTCGTTGGCCTGCGTGCGCAGCTCGCGCAGCACGCGGGCAAGGCGCTGCCGGTCGGCAACCGGAAGCGCCTGGAGTTCGCGGCCTGCTGGAAGCTCCAGCAGCGGATTGATGTAGCCCATGTCGTTCGTCTCTCGCTTCTTGGCCGGCGGTAGCCGGCAGCCTCGCAGAGCAGGATGCCCGTTGAGTGCCGCAGGCGCGAATAAGGGACAGCGAAGATAGATAACCGGCCCGCCGGTTAGCTAACTTCGCACATCCTGCCCGCCTTACGGCGTTGATAACACCAAGGAAAGTCTATACCGAAACCTTCGCATCTGTCTGCATTTAGCGCGAAAAGATGGCGGATATATCGAATGAAGGCGTACAATGCCCGGAAAGCGCGCTAGATGCAGCGGTGTCAAGCTGCTATCCACGCGCTTTACATAGACTTTCCACGGGTCGATACATGAAAATGACGGCTATTTACAAACCACGGAAGCGAGGCGA
TGTCGCTACCCCTTGAGCGCGTGCGCCGTGTGTCGCGCGTAGGTCGCCACGGCTCGCCAGTAGGCGGCCATCGGCCCCTTGCGGCGTGCCCATGCCTTTTCCGCCTCGTCGTTGGCCTGCGTGCGCAGCTCGCGCAGCACGCGGGCAAGGCGCTGCCGGTCGGCAACCGGAAGCGCCTGGAGTTCGCGGCCTGCTGGAAGCTCCAGCAGCGGATTGATGTAGCCCATGTCGTTCGTCTCTCGCTTCTTGGCCGGCGGTAGCCGGCAGCCTCGCAGAGCAGGATGCCCGTTGAGTGCCGCAGGCGCGAATAAGGGACAGCGAAGATAGATAACCGGCCCGCCGGTTAGCTAACTTCGCACATCCTGCCCGCCTTACGGCGTTGATAACACCAAGGAAAGTCTATACCGAAACCTTCGCATCTGTCTGCATTTAGCGCGAAAAGATGGCGGATATATCGAATGAAGGCGTACAATGCCCGGAAAGCGCGCTAGATGCAGCGGTGTCAAGCTGCTATCCACGCGCTTTACATAGACTTTCCACGGGTCGATACATGAAAATGACGGCTATTTACAAACCACGGAAGCGAGGCGA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 931 | GenBank | WP_012478238 |
| Name | TraI_pENH91 |
UniProt ID | A0A1U9V3K4 |
| Length | 752 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 752 a.a. Molecular weight: 82867.55 Da Isoelectric Point: 10.6815
>WP_012478238.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Pseudomonadota]
MIAKHVPMRSLGKSDFAGLVNYVTDAQSKDHRLGQVQITNCDAVSVQDAITEVLATQHTNTRAKGDKTYH
LIVSFRAGEQPSADTLRAIEERICAGLGYGEHQRVSAVHNDTDNLHIHIAINKIHPTRNTMHEPYYSHRA
LAELCTALERDYGLERDNHEPRQRGAEGRAADMEQHAGVESLVGWIKRECLDEIKGAQSWQELHQVMRDN
GLELRERANGLVIEAGDGTMVKASTLARDLSKPKLEARLGPFEASPERQTRTKAKRQYQKDPIRLRVNTV
ELYARYKAEQQTLTATRGQALEQARRRKDRLVEAAKRSGRLRRATIKVIGESGPNKKLLYAQASKALRDE
IQAINKQYQQDRQALYDSHSRRTWADWLKKEATHGNAEALTALRAREAAQGLKGNTIQGRGDPRPGHAPV
TDNITKKGTIIFRAGLTAVRDDGDKLQVSREATREGLQEALRLAMERYGSRITVNGTTQFQAQIIRAAVD
SQLPITFADPALESRRLALLKKENTHERPDRTERADQRPAEHRGRAGRGPGGPGQRAATHDDAARPIAVA
RAGFGRPGGGRAGTAGVHAGAVHRKPDIGRIGRVPPPQSQHRLRTLSQLGVVRIAGGSEVLLPRDVPRHL
EQQGTQPDHQLRRDISRPGSGVAAAPPGLAAADKYIAERESKRSKGFDIPKHARYTAGEGGLAFEGVRNI
EGQALALLKRGDEVMVMPIDQATARRMSRIAVGDAVTITPKGSIKTSKGRSR
MIAKHVPMRSLGKSDFAGLVNYVTDAQSKDHRLGQVQITNCDAVSVQDAITEVLATQHTNTRAKGDKTYH
LIVSFRAGEQPSADTLRAIEERICAGLGYGEHQRVSAVHNDTDNLHIHIAINKIHPTRNTMHEPYYSHRA
LAELCTALERDYGLERDNHEPRQRGAEGRAADMEQHAGVESLVGWIKRECLDEIKGAQSWQELHQVMRDN
GLELRERANGLVIEAGDGTMVKASTLARDLSKPKLEARLGPFEASPERQTRTKAKRQYQKDPIRLRVNTV
ELYARYKAEQQTLTATRGQALEQARRRKDRLVEAAKRSGRLRRATIKVIGESGPNKKLLYAQASKALRDE
IQAINKQYQQDRQALYDSHSRRTWADWLKKEATHGNAEALTALRAREAAQGLKGNTIQGRGDPRPGHAPV
TDNITKKGTIIFRAGLTAVRDDGDKLQVSREATREGLQEALRLAMERYGSRITVNGTTQFQAQIIRAAVD
SQLPITFADPALESRRLALLKKENTHERPDRTERADQRPAEHRGRAGRGPGGPGQRAATHDDAARPIAVA
RAGFGRPGGGRAGTAGVHAGAVHRKPDIGRIGRVPPPQSQHRLRTLSQLGVVRIAGGSEVLLPRDVPRHL
EQQGTQPDHQLRRDISRPGSGVAAAPPGLAAADKYIAERESKRSKGFDIPKHARYTAGEGGLAFEGVRNI
EGQALALLKRGDEVMVMPIDQATARRMSRIAVGDAVTITPKGSIKTSKGRSR
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A1U9V3K4 |
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 65379..76002
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BJN34_RS37730 (BJN34_37425) | 60517..62073 | - | 1557 | WP_011255179 | IS21-like element IS1600 family transposase | - |
| BJN34_RS37735 (BJN34_37430) | 62173..62589 | - | 417 | WP_013397084 | major outer membrane lipoprotein | - |
| BJN34_RS37740 (BJN34_37435) | 62692..63135 | - | 444 | WP_011255142 | OmpA family protein | - |
| BJN34_RS37745 (BJN34_37440) | 63151..63849 | - | 699 | WP_012478194 | TraX family protein | - |
| BJN34_RS37750 (BJN34_37445) | 63846..64115 | - | 270 | WP_011255140 | hypothetical protein | - |
| BJN34_RS37755 (BJN34_37450) | 64145..64780 | - | 636 | WP_011255139 | transglycosylase SLT domain-containing protein | - |
| BJN34_RS37760 (BJN34_37455) | 64782..65360 | - | 579 | WP_011255138 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| BJN34_RS37765 (BJN34_37460) | 65379..67097 | - | 1719 | WP_012478193 | P-type conjugative transfer protein TrbL | virB6 |
| BJN34_RS37770 (BJN34_37465) | 67109..67330 | - | 222 | WP_011255136 | entry exclusion lipoprotein TrbK | - |
| BJN34_RS37775 (BJN34_37470) | 67340..68113 | - | 774 | WP_011255135 | P-type conjugative transfer protein TrbJ | virB5 |
| BJN34_RS37780 (BJN34_37475) | 68130..69509 | - | 1380 | WP_012478192 | TrbI/VirB10 family protein | virB10 |
| BJN34_RS37785 (BJN34_37480) | 69513..69974 | - | 462 | WP_011013267 | lipoprotein | - |
| BJN34_RS37790 (BJN34_37485) | 69977..70870 | - | 894 | WP_014386236 | P-type conjugative transfer protein TrbG | virB9 |
| BJN34_RS37795 (BJN34_37490) | 70888..71676 | - | 789 | WP_011013265 | conjugal transfer protein TrbF | virB8 |
| BJN34_RS37800 (BJN34_37495) | 71673..74231 | - | 2559 | WP_011013264 | VirB4 family type IV secretion/conjugal transfer ATPase | virb4 |
| BJN34_RS37805 (BJN34_37500) | 74228..74539 | - | 312 | WP_011013263 | conjugal transfer protein TrbD | virB3 |
| BJN34_RS37810 (BJN34_37505) | 74543..75007 | - | 465 | WP_011013262 | conjugal transfer system pilin TrbC | virB2 |
| BJN34_RS37815 (BJN34_37510) | 75037..76002 | - | 966 | WP_011013261 | P-type conjugative transfer ATPase TrbB | virB11 |
| BJN34_RS37820 (BJN34_37515) | 76317..76676 | - | 360 | WP_011013260 | transcriptional regulator | - |
| BJN34_RS37825 (BJN34_37520) | 76786..77124 | + | 339 | WP_011013259 | single-stranded DNA-binding protein | - |
Host bacterium
| ID | 1319 | GenBank | NZ_CP017760 |
| Plasmid name | pENH91 | Incompatibility group | IncP(Beta) |
| Plasmid size | 77172 bp | Coordinate of oriT [Strand] | 30494..31084 [-] |
| Host baterium | Cupriavidus necator strain NH9 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | pbrT, pbrA |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |