Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100847
Name   oriT_pSA4 in_silico
Organism   Novosphingobium resinovorum strain SA1
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP017079 (59882..60072 [-], 191 nt)
oriT length   191 nt
IRs (inverted repeats)      IR1: 19..38, 39..58  (AACCGTGAAGCGCGATCCGA..TAGGATTGAGCGTATCGGTT)
  IR2: 119..128, 131..140  (AGCGGTGGCA..TGCCACCGCT)
Location of nic site      86..87
Conserved sequence flanking the
  nic site    		  TATCCTG|C
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 191 nt

>oriT_pSA4
GTGGGGTAGGTATCTCCCAACCGTGAAGCGCGATCCGATAGGATTGAGCGTATCGGTTAACATTAGCCGCCGCAAGGCTTATCCTGCCATAAAGTAGATACCTACCCCACAAACAAAAAGCGGTGGCAACTGCCACCGCTTCAAAATTCCACGCCGCCTCAATGCTGAGTGAACGTGGACGGTAAAAGCTA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   919 GenBank   WP_007686163
Name   TraA_pSA4 insolico UniProt ID   A0A1D8AGX6
Length   1043 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 1043 a.a.        Molecular weight: 115046.74 Da        Isoelectric Point: 6.8987

>WP_007686163.1 MULTISPECIES: Ti-type conjugative transfer relaxase TraA [Sphingomonadaceae]
MAIYHFSAKVISRANGSSAVASAAYRAAERLHDDRLGRDHDFSNKAGVVHSEIMLPEGTPERLNDRATLW
NEVEAGEKRKDAQLAREVEFSIPRELDQKEGIRLARDFVEKQFVERGMVADMNVHWDLGKDGQSKPHAHV
MLSMREVGLEGFGQKVREWNSTALLQEWREAWADHVNERLAELDIDARIDHRSYEAQGIELEPQHKIGPA
ASRMPEQGLEAERVEDHARIARENGEKIIANPQIALDAITRQQATFTRRDLAQFAFRHSDGKDQFDQVMS
AVRTSPELVALGKDGRGEDRFTSREMIDTEQRLERAGYGLADRAGHGLPAASKMGGRDTAERVGLALGSQ
QKDALAHITGKNDLAIVVGYAGTGKSTMLGVARDEWERAGYQVRGAALSGIAAEGLEGGSGIASRTIASM
EYQWEQGRELLGPRDVLVIDEAGMIGTRQMERVLSEAERAGAKVILVGDAEQLQAIEAGAAFRSLAERYG
AAEISDVRRQHEDWQKDATRALATGRTGEAIHAYAEHGMVHAAETREAARESLVEGWDRARIADPDKTRI
ILTHTNAEVRDLNLAARDRLRDAGELGQDVRVSAERGAREFATGDRIMFLKNDRGLGVKNGTLGKVERVS
PDSMAVKLDDGRQVAFDLKDYAHVDHGYAATIHKSQGVTVDRAHVLATPGMDRHSAYVALSRHRDGVQLH
YGRDDFADDRRLVRTLSRERAKDMASDYPMYRDRDAEAQSFADRRGLSGEIRVADAPERKGVEILAPRAG
TSRQMGEDSRTPDIGGDRGAGEAKAAAERQSRRGMFDGLKLSAAPVKAAEPAQGAKEKPAPKRGMFDGLK
LPQRTPTPEPAKELPARAEQGQDRDFRRAVERASRSAEAVLQARASGGPVLEHQKVALERAVQALDTLRP
RASDDMAEAMKRDSGLLREAAAGRSGPMIEAIRQEARVRADPNLRADRFVERWQQLSQDRDRLYRAGDMA
GRKTLGQEMAGMAKSLERDPQVESILRGRTRELGLEIGMSPGRELGRELSRGLGISHDRGLSR

  Protein domains


Predicted by InterproScan.

MobA/MobL family (MobA_MobL)

(18-215)

AAA domain (AAA_30)

(347-534)


  Protein structure


Source ID Structure
AlphaFold DB A0A1D8AGX6


Host bacterium

ID   1307 GenBank   NZ_CP017079
Plasmid name   pSA4 Incompatibility group   _
Plasmid size   67284 bp Coordinate of oriT [Strand]   59882..60072 [-]
Host baterium   Novosphingobium resinovorum strain SA1

Cargo genes

Drug resistance gene   resistance to hydrophobic antibiotics such as nalidixic acid
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -