Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100846
Name   oriT_pPS1 in_silico
Organism   Providencia stuartii strain BE2467
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP017055 (82530..83080 [-], 551 nt)
oriT length   551 nt
IRs (inverted repeats)      IR1: 62..70, 71..79  (AACCCTTTC..GACAGGGTT)
  IR2: 143..150, 154..161  (TGGCCTGC..GGAGGCCA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 551 nt

>oriT_pPS1
TGTAAAGAGGCTGTGAAAGAATAAGAGCATCAAGATTCCAGATAGATAGAGGGAAATTTGACAAATTCCAAAGATGGGTTAGCCTAGTGACAGAACTAGATTCCAGTATTGGAATAATCAGCTTTAAATTCCAGATAGATAGTTATGTGGATAGGAATTGGATAGGAATTGGGAGGGTATTGAGG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   918 GenBank   WP_001326173
Name   TraI_pPS1 insolico UniProt ID   A0A0E3DSB5
Length   990 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 990 a.a.        Molecular weight: 109356.90 Da        Isoelectric Point: 4.9395

>WP_001326173.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure


Source ID Structure
AlphaFold DB A0A0E3DSB5


T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 59800..84946

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BGK56_RS20920 (BGK56_20465) 54927..55835 - 909 WP_000739139 hypothetical protein -
BGK56_RS20925 (BGK56_20470) 55846..56814 - 969 WP_000085162 AAA family ATPase -
BGK56_RS20930 (BGK56_20475) 57034..57219 - 186 WP_001186917 hypothetical protein -
BGK56_RS20935 (BGK56_20480) 57523..57843 - 321 WP_000547566 hypothetical protein -
BGK56_RS20940 (BGK56_20485) 58137..58778 + 642 WP_000796664 hypothetical protein -
BGK56_RS20945 (BGK56_20490) 59120..59761 + 642 WP_223854615 hypothetical protein -
BGK56_RS20950 (BGK56_20495) 59800..62610 - 2811 WP_081368660 conjugal transfer mating pair stabilization protein TraN traN
BGK56_RS20955 (BGK56_20500) 62714..63718 - 1005 WP_005507569 TraU family protein traU
BGK56_RS20960 (BGK56_20505) 63718..64389 - 672 WP_000575345 EAL domain-containing protein -
BGK56_RS20965 (BGK56_20510) 64386..65651 - 1266 WP_001447719 TrbC family F-type conjugative pilus assembly protein traW
BGK56_RS20970 (BGK56_20515) 65614..66144 - 531 WP_001010740 S26 family signal peptidase -
BGK56_RS20975 (BGK56_20520) 66141..66458 - 318 WP_000351984 hypothetical protein -
BGK56_RS20980 (BGK56_20525) 66473..68920 - 2448 WP_020833628 type IV secretion system protein TraC virb4
BGK56_RS20985 (BGK56_20530) 68917..69624 - 708 WP_001259346 DsbC family protein -
BGK56_RS20990 (BGK56_20535) 69773..75259 - 5487 WP_072503044 Ig-like domain-containing protein -
BGK56_RS20995 (BGK56_20540) 75460..75852 - 393 WP_000479535 TraA family conjugative transfer protein -
BGK56_RS21000 (BGK56_20545) 75856..76434 - 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
BGK56_RS21005 (BGK56_20550) 76431..77744 - 1314 WP_024131605 TraB/VirB10 family protein traB
BGK56_RS21010 (BGK56_20555) 77744..78661 - 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
BGK56_RS21015 (BGK56_20560) 78645..79271 - 627 WP_001049717 TraE/TraK family type IV conjugative transfer system protein traE
BGK56_RS21020 (BGK56_20565) 79268..79549 - 282 WP_000805625 type IV conjugative transfer system protein TraL traL
BGK56_RS21025 (BGK56_20570) 79694..80059 - 366 WP_001052531 hypothetical protein -
BGK56_RS22855 80071..80214 - 144 WP_193407049 hypothetical protein -
BGK56_RS21030 (BGK56_20575) 80395..81057 - 663 WP_001231464 hypothetical protein -
BGK56_RS21035 (BGK56_20580) 81057..81434 - 378 WP_000869297 hypothetical protein -
BGK56_RS23820 81676..81852 - 177 WP_169342285 hypothetical protein -
BGK56_RS21045 (BGK56_20585) 81900..82529 - 630 WP_000743449 DUF4400 domain-containing protein tfc7
BGK56_RS23825 (BGK56_20590) 82486..83031 - 546 WP_000228720 hypothetical protein -
BGK56_RS21055 (BGK56_20595) 83081..84946 - 1866 WP_000178857 conjugative transfer system coupling protein TraD virb4
BGK56_RS21060 (BGK56_20600) 84943..87915 - 2973 WP_001326173 MobH family relaxase -
BGK56_RS21065 (BGK56_20605) 88083..88700 + 618 WP_001249395 hypothetical protein -
BGK56_RS21070 (BGK56_20610) 88682..88915 + 234 WP_001191890 hypothetical protein -

Host bacterium

ID   1306 GenBank   NZ_CP017055
Plasmid name   pPS1 Incompatibility group   IncA/C2
Plasmid size   195378 bp Coordinate of oriT [Strand]   82530..83080 [-]
Host baterium   Providencia stuartii strain BE2467

Cargo genes

Drug resistance gene   sul2, aph(3')-Ia
Virulence gene   ureG
Metal resistance gene   arsH
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -