Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100845
Name   oriT_p23-1 in_silico
Organism   Klebsiella pneumoniae isolate 23
Sequence Completeness      core
NCBI accession of oriT (coordinates [strand])   NZ_CP016927 (14982..15086 [-], 105 nt)
oriT length   105 nt
IRs (inverted repeats)      18..35, 42..59  (GTTTTTGGTACACCGCCG..CGGCAGTGACGCAAAAAC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 105 nt

>oriT_p23-1
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   917 GenBank   WP_004206924
Name   Putativerelaxaseofp23-1 insolico UniProt ID   A0A0U3I0I2
Length   659 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 659 a.a.        Molecular weight: 75087.84 Da        Isoelectric Point: 10.0285

>WP_004206924.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNPK

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(86-334)


  Protein structure


Source ID Structure
AlphaFold DB A0A0U3I0I2


T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 600..12184

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A8C23_RS26695 (A8C23_26695) 60..554 - 495 WP_004187480 hypothetical protein -
A8C23_RS26700 (A8C23_26700) 600..989 - 390 WP_004187479 DUF6750 family protein traR
A8C23_RS26705 (A8C23_26705) 1006..1536 - 531 WP_004187478 conjugal transfer protein TraQ traQ
A8C23_RS26710 (A8C23_26710) 1560..2264 - 705 WP_004206933 conjugal transfer protein TraP traP
A8C23_RS26715 (A8C23_26715) 2276..3625 - 1350 WP_036970781 conjugal transfer protein TraO traO
A8C23_RS26720 (A8C23_26720) 3637..4788 - 1152 WP_015062843 DotH/IcmK family type IV secretion protein traN
A8C23_RS26730 (A8C23_26730) 4797..5510 - 714 WP_223294958 DotI/IcmL family type IV secretion protein traM
A8C23_RS26735 (A8C23_26735) 5578..5988 + 411 WP_004187465 H-NS family nucleoid-associated regulatory protein -
A8C23_RS28360 6017..6172 + 156 WP_172693602 Hha/YmoA family nucleoid-associated regulatory protein -
A8C23_RS26740 (A8C23_26740) 6173..6685 - 513 WP_011091071 hypothetical protein traL
A8C23_RS30560 6651..9956 - 3306 WP_220381329 LPD7 domain-containing protein -
A8C23_RS26750 (A8C23_26750) 9981..10241 - 261 WP_004187310 IcmT/TraK family protein traK
A8C23_RS26755 (A8C23_26755) 10231..11394 - 1164 WP_004206926 plasmid transfer ATPase TraJ virB11
A8C23_RS26760 (A8C23_26760) 11405..12184 - 780 WP_004187315 type IV secretory system conjugative DNA transfer family protein traI
A8C23_RS26765 (A8C23_26765) 12181..12681 - 501 WP_004206925 DotD/TraH family lipoprotein -
A8C23_RS26770 (A8C23_26770) 12695..14674 - 1980 WP_004206924 TraI/MobA(P) family conjugative relaxase -
A8C23_RS26775 (A8C23_26775) 14661..14978 - 318 WP_004206923 plasmid mobilization protein MobA -
A8C23_RS26780 (A8C23_26780) 15254..15619 + 366 WP_069012985 MobC -
A8C23_RS26785 (A8C23_26785) 16120..16656 - 537 WP_036970776 hypothetical protein -
A8C23_RS26790 (A8C23_26790) 16789..17100 - 312 WP_004187333 hypothetical protein -

Host bacterium

ID   1305 GenBank   NZ_CP016927
Plasmid name   p23-1 Incompatibility group   IncL/M
Plasmid size   72093 bp Coordinate of oriT [Strand]   14982..15086 [-]
Host baterium   Klebsiella pneumoniae isolate 23

Cargo genes

Drug resistance gene   aph(6)-Id, aph(3'')-Ib, aph(3')-VIb, blaCTX-M-14b, blaOXA-48
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -