Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100843
Name   oriT_pJM1B in_silico
Organism   Lactococcus cremoris strain JM1
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP016748 (37631..38090 [+], 460 nt)
oriT length   460 nt
IRs (inverted repeats)      IR1: 138..147, 152..161  (CCCATTTTAT..ATAAAATGGG)
  IR2: 207..219, 223..235  (AAAAGGGGAAAGT..ACTTCCCCCTTTT)
  IR3: 242..255, 268..281  (ACATTGTAATACAA..TTGTATTACAATGT)
Location of nic site      290..291
Conserved sequence flanking the
  nic site    		  CTTG|CC
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 460 nt

>oriT_pJM1B
TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGGAAAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTCTTCTTTTTGTATGACAAAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGTAGGTGGCGTTTGCATAAAGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCAACATTGTAATACAAGAACGAAGTGATTTGTATTACAATGTAATAGCTTGCCAGTATTTATGGTTTTATATGGTCTATTTTGTTGTGAGGATTGTAACCGAATAGGGCGCAATGCTTATTACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGAGGCATTTTATCTTTGAGGAAGTTCTTGATGGATCAGAAAAATGTATCACAAATTTAAAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   915 GenBank   WP_050595623
Name   PriA_pJM1B insolico UniProt ID   _
Length   380 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 380 a.a.        Molecular weight: 44959.99 Da        Isoelectric Point: 9.0637

>WP_050595623.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Lactococcus]
MVLNATNPLTLNKFQQNKNDLERLKEISDNISKEYGCKIIVRPEQKLGNSHKNYLVYLAKNSYRKEIKNK
LDFLMNHSHTWEDFKEKARALNLKVDDTKKYTTYLLEGSEQTKKIRDRSLKNDKFLKENLKERIEKNTIG
YSVDEVVKLWKDKESIQEKGREKEIEILLEHWQVTKETEKDLVVTIDTAFDNEATIKIPARCVDKLENGQ
YKIFIKKGDRFSYLDKKSPANHKIMYGATVAKNLQRQSGNIPLYSDNVNIKLKQVFHEFDFLISQGLSFD
RSFETIGEELKATYQETQHQLDKLDTKILEYVETTKTLPYEDTSIRDTIKNLTKERDDLRDTLYKVDKNI
QYYQKSEQRLEAYQKNQSPKHKARDDDFEI

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(2-136)


  Protein structure



No available structure.




Host bacterium

ID   1303 GenBank   NZ_CP016748
Plasmid name   pJM1B Incompatibility group   _
Plasmid size   48282 bp Coordinate of oriT [Strand]   37631..38090 [+]
Host baterium   Lactococcus cremoris strain JM1

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -