Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100842
Name   oriT_pJM3C in_silico
Organism   Lactococcus cremoris strain JM3
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP016739 (13398..13879 [-] [-], 482 nt)
oriT length   482 nt
IRs (inverted repeats)      IR1: 21..33, 37..49  (AAAAGGGGAAAGT..ACTTCCCCCTTTT)
 IR2: 55..69, 82..96  (CACATTGTAATACAA..TTGTATTACAATGTG)
Location of nic site      104..105
Conserved sequence flanking the
  nic site    		  CTTG|CA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 482 nt

>oriT_pJM3C
CGACACACTCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTATGGGTTTATATTTCCTATTTTGTTGTGAGGATTGTAACCGAATAGGGCGCAATACTTATTACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGAGGCATTTTATCTTTGAGGAAGTTCTTGATGGATCAGAAAAATGTATCACAAATTTAAACAAAGACTCACTCATTTAAGAGACGCTACTAGCATGAAATTTTGTTGTTGCGATAAGCAACTTCTGATACACGATTTTTAGCCATTACATCACTCGTTTTTAGAGTGATGTGTAAGTGCGCATTGCACTCTTTTTTAACGAAACAAGCCGACCAGCGTTTGAAACTTTTTAGTTTTTCATCATTCTATTTTAAAACGCTCTAAAACTCGA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   914 GenBank   WP_063284081
Name   Relaxase_pJM3C insolico UniProt ID   A0A1V0P6Q0
Length   410 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 410 a.a.        Molecular weight: 47744.33 Da        Isoelectric Point: 8.1264

>WP_063284081.1 relaxase/mobilization nuclease domain-containing protein [Lactococcus cremoris]
MATIAKISNGASAASALNYALGQDRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGIDPFIAKEQFD
VIRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLYPNHQSAVYTHLDGKNHVLHN
HIIVNKVNLETGKKLREQKGESVQRAREMNDRLASRENWHILEPPKERQTETEKELIAKNEYSYMDDLRE
RINKSLQDVSVSSYETFKERLSDNGVILSERGQTFSYAFLDANNKQRRARETRLGSDFGRETILHELENR
ARQNEFRAVEQREPAITPLERDTQQRESEIVSLEQAIEPRKSEALKRESTINRFIDTIKQFAGRVPELTQ
RVTRKLKQTKDKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(9-280)


  Protein structure



No available structure.




Host bacterium

ID   1302 GenBank   NZ_CP016739
Plasmid name   pJM3C Incompatibility group   _
Plasmid size   54483 bp Coordinate of oriT [Strand]   13398..13879 [-] [-]
Host baterium   Lactococcus cremoris strain JM3

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -