Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100841 |
| Name | oriT_pJM3A  |
| Organism | Lactococcus cremoris strain JM3 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP016737 (10136..10598 [-], 463 nt) |
| oriT length | 463 nt |
| IRs (inverted repeats) | IR1: 138..147, 152..161 (CCCATTTTAT..ATAAAATGGG) IR2: 207..219, 223..235 (AAAAGGGGAAAGT..ACTTCCCCCTTTT) IR3: 242..255, 268..281 (ACATTGTAATACAA..TTGTATTACAATGT) |
| Location of nic site | 290..291 |
| Conserved sequence flanking the nic site |
CTTG|CA |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 463 nt
>oriT_pJM3A
TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGGAAAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTCTTCTTTTTGTATGACAAAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGTAGGTGGCGTTTGCGTAAAGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCAACATTGTAATACAAGAACGAAGTGATTTGTATTACAATGTGATAGCTTGCAGTATTTATAGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATACTTATTACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGAGGCATTTTATCTTTGAGTAAGTTATTGATGGATCAGAAAAATGTATCACAAATTGAAACAAAG
TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGGAAAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTCTTCTTTTTGTATGACAAAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGTAGGTGGCGTTTGCGTAAAGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCAACATTGTAATACAAGAACGAAGTGATTTGTATTACAATGTGATAGCTTGCAGTATTTATAGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATACTTATTACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGAGGCATTTTATCTTTGAGTAAGTTATTGATGGATCAGAAAAATGTATCACAAATTGAAACAAAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 913 | GenBank | WP_011669060 |
| Name | Relaxase_pJM3A |
UniProt ID | A0A1V0P5P3 |
| Length | 135 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 135 a.a. Molecular weight: 15149.12 Da Isoelectric Point: 6.2189
>WP_011669060.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Lactococcus]
MTVIYMPRDTSGVIHSQGQLSRALNYIVNPEKTKGGELVSGQNINVPSNAYNEMLLTREMAILAGNQPKE
NERFGFHFVQSFSPEDNLTPEQVHEIGLKTMKAYLGESAEFVIATHTDKEHLHNVRPDRVLSQVV
MTVIYMPRDTSGVIHSQGQLSRALNYIVNPEKTKGGELVSGQNINVPSNAYNEMLLTREMAILAGNQPKE
NERFGFHFVQSFSPEDNLTPEQVHEIGLKTMKAYLGESAEFVIATHTDKEHLHNVRPDRVLSQVV
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Host bacterium
| ID | 1301 | GenBank | NZ_CP016737 |
| Plasmid name | pJM3A | Incompatibility group | _ |
| Plasmid size | 75814 bp | Coordinate of oriT [Strand] | 10136..10598 [-] |
| Host baterium | Lactococcus cremoris strain JM3 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |