Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100840
Name   oriT_pUC08C in_silico
Organism   Lactococcus lactis subsp. lactis strain UC08
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP016728 (10604..11066 [+], 463 nt)
oriT length   463 nt
IRs (inverted repeats)      IR1: 138..147, 152..161  (CCCATTTTAT..ATAAAATGGG)
  IR2: 207..219, 223..235  (AAAAGGGGAAAGT..ACTGCCCCCTTTT)
  IR3: 241..255, 268..282  (CACATTGTAATACAA..TTGTATTACAATGTG)
Location of nic site      290..291
Conserved sequence flanking the
  nic site    		  CTTG|CA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 463 nt

>oriT_pUC08C
TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGAAAAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTCTTCTTTTTGTATGACAAAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGTAGGTGGCGTTTGCGTAAAGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTGCCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTGTTTTGTATTACAATGTGATAGCTTGCAGTATTTATGGATTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATGCTTATTACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATTTTATCTTTGAGGAGGCTATTTATGGATCAGAAAGAAGTATCACAAAATCAAACAAAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   912 GenBank   WP_081200409
Name   Relaxase_pUC08C insolico UniProt ID   _
Length   410 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 410 a.a.        Molecular weight: 47620.24 Da        Isoelectric Point: 9.0715

>WP_081200409.1 relaxase/mobilization nuclease domain-containing protein [Lactococcus lactis]
MATIAKISNGASAASALNYALGQDRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGIDPFIAKEQFD
VVRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLYPNHQSAVYTHLDGKNHVLHN
HIIVNKVNLETGNKLREQKGESVQRAREMNDRLASRENWHILEPPKERQTETEKELIAKNEYSYMDDLRE
RINKSLQDISVGSYETFKERLSDNGVILSERGQTFSYAFLDANNKQRRARETRLGSDFGRETILHELENR
ARQNEFRAVEQREPAITPLERDTQQRESEIVSLEQAIEPRKSEALKRESTIDRFIDTLKQLAGRVPALTQ
RVTRKLNQTKEKILDDFERRFSKDMKNYEKEQQKNLEKQTNRAVQSEKKPTKNHNRGMSR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(9-280)


  Protein structure



No available structure.




Host bacterium

ID   1300 GenBank   NZ_CP016728
Plasmid name   pUC08C Incompatibility group   _
Plasmid size   15398 bp Coordinate of oriT [Strand]   10604..11066 [+]
Host baterium   Lactococcus lactis subsp. lactis strain UC08

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -