Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100838
Name   oriT_pUC11C in_silico
Organism   Lactococcus lactis subsp. lactis strain UC11
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP016722 (15730..16191 [+], 462 nt)
oriT length   462 nt
IRs (inverted repeats)      IR1: 137..146, 151..160  (CCCATTTTAT..ATAAAATGGG)
  IR2: 206..218, 222..234  (AAAAGGGGAAAGT..ACTTCCCCCTTTT)
  IR3: 240..254, 267..281  (CACATTGTAATACAA..TTGTATTACAATGTG)
Location of nic site      289..290
Conserved sequence flanking the
  nic site    		  CTTG|CA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 462 nt

>oriT_pUC11C
AGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGAAAAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTTCTTCTTTTTGTATGACAAAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGTAGGTGGCGTTTGCGTAAAGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTATGGTTTTATATTTGCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATGCTTATTACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATTTTATCTTTGAGGAGGCTATTTATGGATCAGAAAGAAGTATCACAAAATCAAACAAAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   910 GenBank   WP_082225095
Name   Relaxase_pUC11C insolico UniProt ID   _
Length   410 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 410 a.a.        Molecular weight: 47812.45 Da        Isoelectric Point: 8.6734

>WP_082225095.1 relaxase/mobilization nuclease domain-containing protein [Lactococcus lactis]
MATIAKISNGASAASALNYALGQDRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGIDPFIAKEQFD
VVRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLYPNHQSAVYTHLDGKNHVLHN
HIIVNKVNLETGKKLREQKGESVQRAREMNDRLASRENWHILEPPKERQTETEKELIAKNEYSYMDDLRE
RINKSLQDLSVSSYETFKERLSDNGVILSERGQTFSYAFLDANNKQRRARETRLGSDFGRETILHELENR
ARQNEFRAVEQREPAITPLERDTQQRESEIVNLEQAIEPRKSEALKRESKINRFIDTIKQFAGRVPELTQ
RVTRKLKQTKEKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(9-280)


  Protein structure



No available structure.




Host bacterium

ID   1298 GenBank   NZ_CP016722
Plasmid name   pUC11C Incompatibility group   _
Plasmid size   19351 bp Coordinate of oriT [Strand]   15730..16191 [+]
Host baterium   Lactococcus lactis subsp. lactis strain UC11

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -