Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100837 |
| Name | oriT_pUC063B  |
| Organism | Lactococcus lactis subsp. lactis strain UC063 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP016716 (26886..27349 [-], 464 nt) |
| oriT length | 464 nt |
| IRs (inverted repeats) | IR1: 139..148, 153..162 (CCCATTTTAT..ATAAAATGGG) IR2: 208..220, 224..236 (AAAAGGGGAAAGT..ACTTCCCCCTTTT) IR3: 242..256, 269..283 (CACATTGTAATACAA..TTGTATTACAATGTG) |
| Location of nic site | 291..292 |
| Conserved sequence flanking the nic site |
CTTG|CA |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 464 nt
>oriT_pUC063B
TTAGTGGATGATCAAACAAAATACGAGAGTTTTTTTGTTCGTTCATCCATGGTTTTAGAAAAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTTCTTCTTTTTGTATGACAAAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGCAGGTGGCGTTTGCGTAAAGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAGAACGTAGTAATTTGTATTACAATGTGATAGCTTGCAGTATTTATGGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATGCTTATTACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATTTTATCTTTTAGGAGGCTATTTATGGATCAGAAAGAAGTATCACAAAATCAAACAAAG
TTAGTGGATGATCAAACAAAATACGAGAGTTTTTTTGTTCGTTCATCCATGGTTTTAGAAAAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTTCTTCTTTTTGTATGACAAAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGCAGGTGGCGTTTGCGTAAAGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAGAACGTAGTAATTTGTATTACAATGTGATAGCTTGCAGTATTTATGGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATGCTTATTACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATTTTATCTTTTAGGAGGCTATTTATGGATCAGAAAGAAGTATCACAAAATCAAACAAAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 909 | GenBank | WP_081196304 |
| Name | PriA_pUC063B |
UniProt ID | _ |
| Length | 505 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 505 a.a. Molecular weight: 59137.01 Da Isoelectric Point: 8.3720
>WP_081196304.1 relaxase/mobilization nuclease domain-containing protein [Lactococcus lactis]
MTVIYMPKQSNGTVHSAKDLKQLIDYVMNSEKTNDFEYVSGQNILDIHSTCDEMLATRTMANALKKKPQK
NERFGYHFVQSFSPDDHLTPEQVHEIGCKTMKEYLGSSAEFIIATHTDKPHLHNHIVLNATDPLTLNKFQ
QNKNDLERLKEISDNISKEYGCKIIVRPEQKLGNSHKNYLVYLAKNSYRKEIKNKLEFLVNHSHTWEDFK
TKARALNLKVDDTKKYTTYLLEGSEQTKKIRDRSLKNDKFLKENLKERIEKNTIGYSVEEVVKLWNDKES
IQEKGRKKEIEILLEHWQVTKETEKDLVVTIDTAFDNEATIKIPARCVDKLENGQYKIFIKKGDRFSYID
KRSPANHKIMVGATVAKNLQRQSGNIPLYSDNVNIKLKQVFHEFDFLISQGLGFDRSFETIGEELKATYQ
ETQHQLDKLDTKILEYVETTKTLPYEDTSIRDTIKNLTKERDDLRDTLYKVDKNIQYYQKSEQRLEAYQK
NQSPKHKARDDDFEI
MTVIYMPKQSNGTVHSAKDLKQLIDYVMNSEKTNDFEYVSGQNILDIHSTCDEMLATRTMANALKKKPQK
NERFGYHFVQSFSPDDHLTPEQVHEIGCKTMKEYLGSSAEFIIATHTDKPHLHNHIVLNATDPLTLNKFQ
QNKNDLERLKEISDNISKEYGCKIIVRPEQKLGNSHKNYLVYLAKNSYRKEIKNKLEFLVNHSHTWEDFK
TKARALNLKVDDTKKYTTYLLEGSEQTKKIRDRSLKNDKFLKENLKERIEKNTIGYSVEEVVKLWNDKES
IQEKGRKKEIEILLEHWQVTKETEKDLVVTIDTAFDNEATIKIPARCVDKLENGQYKIFIKKGDRFSYID
KRSPANHKIMVGATVAKNLQRQSGNIPLYSDNVNIKLKQVFHEFDFLISQGLGFDRSFETIGEELKATYQ
ETQHQLDKLDTKILEYVETTKTLPYEDTSIRDTIKNLTKERDDLRDTLYKVDKNIQYYQKSEQRLEAYQK
NQSPKHKARDDDFEI
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Host bacterium
| ID | 1297 | GenBank | NZ_CP016716 |
| Plasmid name | pUC063B | Incompatibility group | _ |
| Plasmid size | 38269 bp | Coordinate of oriT [Strand] | 26886..27349 [-] |
| Host baterium | Lactococcus lactis subsp. lactis strain UC063 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |