Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100836
Name   oriT_p275B in_silico
Organism   Lactococcus lactis subsp. lactis strain 275
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP016700 (40221..40684 [+], 464 nt)
oriT length   464 nt
IRs (inverted repeats)      IR1: 139..148, 153..162  (CCCATTTTAT..ATAAAATGGG)
  IR2: 208..220, 224..236  (AAAAGGGGAAAGT..ACTTCCCCCTTTT)
  IR3: 242..256, 269..283  (CACATTGTAATACAA..TTGTATTACAATGTG)
Location of nic site      291..292
Conserved sequence flanking the
  nic site    		  CTTG|CA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 464 nt

>oriT_p275B
TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGAAAAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTTCTTCTTTTTGTATGACAAAAAGAAAGATTTTTTGCCCATTTTATTTTTATAAAATGGGTAGGTGGCGTTTGCGTAAAGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTAAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTGATTTGTATTACAATGTGATAGCTTGCAGTATTTATGGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATGCTTATTACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATTTTATCTTTGAGGAGGCTATTTATGGATCAGAAAAATGTATCACAAAATCAAACAAAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   908 GenBank   WP_081144421
Name   PriA_p275B insolico UniProt ID   _
Length   505 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 505 a.a.        Molecular weight: 59172.87 Da        Isoelectric Point: 7.2580

>WP_081144421.1 relaxase/mobilization nuclease domain-containing protein [Lactococcus lactis]
MTVIKMPKQSNGTVHSSKDLNQLIDYVMNPEKTNDFEYVSGQNILDIHSTCDEMLATRTMANALKNKPRK
NERFGYHFVQSFSPDDHLTPEQAHEIGLKTMKEYLGNSAEFIIATHTDKPHLHNHIVLNATDPLTLNKFQ
QSKNDLERLKEISDNISKEYGCKIIDRPEQKLGNSHKNYLVYLAKNSYRKEIKNKLEFLMNHSHTWEDFK
EKARALNLKVDDTKKYTTYLLEGSEQTKKIRDRSLKNDKFLKENLKERIEKNTIGYSVEEVVKLWKDKES
IQEKGQEKEIEILLEHWQVTKETEKDLVVTIDTAFDNEATIKIPARCVDKLENGQYKIFIKKGDRFSYID
QRSPANHKIMVGATVAKNLQRQSGNIPLYSDNVNIKLKQVFHEFDFLISQGLGFDRSFETIGEELKATYQ
ETQHQLDKLDTKILEYVETTKTLPYEDTSIRDTIKNLTKERDDLRDTLYKVDKNIQYYQKSEQRLEAYQK
NQSPKHKARDDDFEI

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(17-261)


  Protein structure



No available structure.




Host bacterium

ID   1296 GenBank   NZ_CP016700
Plasmid name   p275B Incompatibility group   _
Plasmid size   56332 bp Coordinate of oriT [Strand]   40221..40684 [+]
Host baterium   Lactococcus lactis subsp. lactis strain 275

Cargo genes

Drug resistance gene   -
Virulence gene   cpsF
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA21