Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100830 |
| Name | oriT_pSA01AB09084001_92  |
| Organism | Salmonella enterica subsp. enterica serovar Heidelberg strain SA01AB09084001 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP016533 (38105..38214 [-], 110 nt) |
| oriT length | 110 nt |
| IRs (inverted repeats) | IR1: 24..31, 35..42 (GTCGGGGC..GCCCTGAC) IR2: 49..65, 72..88 (GCAATTGTAATAGCGTC..GACGGTATTACAATTGC) |
| Location of nic site | 96..97 |
| Conserved sequence flanking the nic site |
CATCCTG|T |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 110 nt
>oriT_pSA01AB09084001_92
TCACTTCAGGCTCCTTACGGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTCAGG
TCACTTCAGGCTCCTTACGGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTCAGG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 902 | GenBank | WP_010891268 |
| Name | Relaxase_pSA01AB09084001_92 |
UniProt ID | _ |
| Length | 899 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 899 a.a. Molecular weight: 103977.42 Da Isoelectric Point: 7.2753
>WP_010891268.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYIAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFLQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYIAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFLQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 43574..81873
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BAT52_RS23430 (BAT52_23205) | 41290..43581 | - | 2292 | WP_001289276 | F-type conjugative transfer protein TrbC | - |
| BAT52_RS23435 (BAT52_23210) | 43574..44644 | - | 1071 | WP_000151583 | IncI1-type conjugal transfer protein TrbB | trbB |
| BAT52_RS23440 (BAT52_23215) | 44663..45871 | - | 1209 | WP_000121273 | IncI1-type conjugal transfer protein TrbA | trbA |
| BAT52_RS23445 (BAT52_23220) | 46163..46315 | + | 153 | WP_001303307 | Hok/Gef family protein | - |
| BAT52_RS23450 (BAT52_23225) | 46387..46638 | - | 252 | WP_001291964 | hypothetical protein | - |
| BAT52_RS26500 | 47139..47234 | + | 96 | WP_000609148 | DinQ-like type I toxin DqlB | - |
| BAT52_RS26145 | 47299..47475 | - | 177 | WP_001054904 | hypothetical protein | - |
| BAT52_RS23460 (BAT52_23235) | 47867..48076 | + | 210 | WP_000062603 | HEAT repeat domain-containing protein | - |
| BAT52_RS23465 (BAT52_23240) | 48148..48810 | - | 663 | WP_000653334 | plasmid IncI1-type surface exclusion protein ExcA | - |
| BAT52_RS23470 (BAT52_23245) | 48875..51037 | - | 2163 | WP_000698351 | DotA/TraY family protein | traY |
| BAT52_RS23475 (BAT52_23250) | 51134..51718 | - | 585 | WP_001037987 | IncI1-type conjugal transfer protein TraX | - |
| BAT52_RS23480 (BAT52_23255) | 51747..52949 | - | 1203 | WP_001189160 | IncI1-type conjugal transfer protein TraW | traW |
| BAT52_RS25205 | 52916..53530 | - | 615 | WP_000337398 | IncI1-type conjugal transfer protein TraV | traV |
| BAT52_RS23485 (BAT52_23260) | 53530..56574 | - | 3045 | WP_001024780 | IncI1-type conjugal transfer protein TraU | traU |
| BAT52_RS23490 (BAT52_23265) | 56664..57464 | - | 801 | WP_001164788 | IncI1-type conjugal transfer protein TraT | traT |
| BAT52_RS23495 (BAT52_23270) | 57448..57636 | - | 189 | WP_001277255 | putative conjugal transfer protein TraS | - |
| BAT52_RS23500 (BAT52_23275) | 57700..58104 | - | 405 | WP_000086957 | IncI1-type conjugal transfer protein TraR | traR |
| BAT52_RS23505 (BAT52_23280) | 58155..58682 | - | 528 | WP_001055569 | conjugal transfer protein TraQ | traQ |
| BAT52_RS23510 (BAT52_23285) | 58682..59386 | - | 705 | WP_000801920 | IncI1-type conjugal transfer protein TraP | traP |
| BAT52_RS23515 (BAT52_23290) | 59386..60675 | - | 1290 | WP_001272005 | conjugal transfer protein TraO | traO |
| BAT52_RS23520 (BAT52_23295) | 60678..61661 | - | 984 | WP_001191877 | IncI1-type conjugal transfer protein TraN | traN |
| BAT52_RS23525 (BAT52_23300) | 61672..62364 | - | 693 | WP_000138548 | DotI/IcmL family type IV secretion protein | traM |
| BAT52_RS23530 (BAT52_23305) | 62361..62708 | - | 348 | WP_001055900 | conjugal transfer protein | traL |
| BAT52_RS23535 (BAT52_23310) | 62726..66493 | - | 3768 | WP_001141529 | LPD7 domain-containing protein | - |
| BAT52_RS23540 (BAT52_23315) | 66583..67134 | - | 552 | WP_000014583 | phospholipase D family protein | - |
| BAT52_RS25210 | 67149..67439 | - | 291 | WP_001299214 | hypothetical protein | traK |
| BAT52_RS23545 (BAT52_23320) | 67436..68584 | - | 1149 | WP_001024972 | plasmid transfer ATPase TraJ | virB11 |
| BAT52_RS23550 (BAT52_23325) | 68581..69399 | - | 819 | WP_000646098 | IncI1-type conjugal transfer lipoprotein TraI | traI |
| BAT52_RS23555 (BAT52_23330) | 69396..69854 | - | 459 | WP_001079808 | IncI1-type conjugal transfer lipoprotein TraH | - |
| BAT52_RS23560 (BAT52_23335) | 70249..70833 | - | 585 | WP_000977522 | histidine phosphatase family protein | - |
| BAT52_RS23565 (BAT52_23340) | 70893..72095 | - | 1203 | WP_000976351 | conjugal transfer protein TraF | - |
| BAT52_RS23570 (BAT52_23345) | 72180..73004 | - | 825 | WP_001238939 | conjugal transfer protein TraE | traE |
| BAT52_RS23575 (BAT52_23350) | 73155..74309 | - | 1155 | WP_001139957 | site-specific integrase | - |
| BAT52_RS26010 | 75830..76078 | + | 249 | WP_001349157 | hypothetical protein | - |
| BAT52_RS23585 (BAT52_23360) | 76075..77367 | - | 1293 | WP_001417545 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| BAT52_RS25245 | 77367..78023 | - | 657 | WP_001193553 | prepilin peptidase | - |
| BAT52_RS23590 (BAT52_23365) | 78008..78568 | - | 561 | WP_000014116 | lytic transglycosylase domain-containing protein | virB1 |
| BAT52_RS23595 (BAT52_23370) | 78578..79192 | - | 615 | WP_065643134 | type 4 pilus major pilin | - |
| BAT52_RS23600 (BAT52_23375) | 79210..80307 | - | 1098 | WP_001208805 | type II secretion system F family protein | - |
| BAT52_RS23605 (BAT52_23380) | 80320..81873 | - | 1554 | WP_000362202 | ATPase, T2SS/T4P/T4SS family | virB11 |
| BAT52_RS23610 (BAT52_23385) | 81884..82336 | - | 453 | WP_001247336 | type IV pilus biogenesis protein PilP | - |
| BAT52_RS23615 (BAT52_23390) | 82323..83618 | - | 1296 | WP_000752774 | type 4b pilus protein PilO2 | - |
| BAT52_RS23620 (BAT52_23395) | 83611..85293 | - | 1683 | WP_000748143 | PilN family type IVB pilus formation outer membrane protein | - |
| BAT52_RS23625 (BAT52_23400) | 85307..85744 | - | 438 | WP_000539807 | type IV pilus biogenesis protein PilM | - |
| BAT52_RS25250 | 85744..86811 | - | 1068 | WP_000742600 | type IV pilus biogenesis lipoprotein PilL | - |
Host bacterium
| ID | 1290 | GenBank | NZ_CP016533 |
| Plasmid name | pSA01AB09084001_92 | Incompatibility group | IncI1 |
| Plasmid size | 91980 bp | Coordinate of oriT [Strand] | 38105..38214 [-] |
| Host baterium | Salmonella enterica subsp. enterica serovar Heidelberg strain SA01AB09084001 |
Cargo genes
| Drug resistance gene | resistance to cetylpyridinium; quaternary ammonium compound resistance |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |