Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100824
Name   oriT_pSH14-028_6 in_silico
Organism   Salmonella enterica subsp. enterica serovar Heidelberg strain SH14-028
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP016513 (6273..6355 [-], 83 nt)
oriT length   83 nt
IRs (inverted repeats)      3..12, 16..25  (GTGTCGGGGC..GCCCTGACCC)
Location of nic site      56..57
Conserved sequence flanking the
  nic site    		  CTGG|CTTA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 83 nt

>oriT_pSH14-028_6
GGGTGTCGGGGCGCAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACCATGCGGCATCAGTGCGGATT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   896 GenBank   WP_000635804
Name   Nuclease_pSH14-028_6 insolico UniProt ID   _
Length   517 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 517 a.a.        Molecular weight: 57794.27 Da        Isoelectric Point: 6.0811

>WP_000635804.1 MULTISPECIES: nuclease [Enterobacteriaceae]
MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSFAEKELPPGGR
EKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLVN
HHYGLHDPNAPENRRTLTLPDNLPETKQALAEGVTRGIDALYHAGEIKGRQDVIQALTEAGLEVVRVTRS
SISIADPNGGKNIRLKGAFYEQSFADGRGVREKAERESRIYRENAEQRVQEARRICKRGCDIKRDENQRR
YSPVHSLDRGIAGKTPGRGERGDDAAQEGRVKAGREYGHDVTGDSLSPVYREWRDALVSWREDTGEPGRN
QEAGRDIAETEREDMGRGVCAGREQEIPCPSVREISGGDSLSGERVGTSEGVTQSDRAGNTFAERLRAAA
TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQRELEIREERLIQE
REHVLSLERERQPEIQERTLDGPSLGW

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(57-237)


  Protein structure



No available structure.




Host bacterium

ID   1284 GenBank   NZ_CP016513
Plasmid name   pSH14-028_6 Incompatibility group   ColRNAI
Plasmid size   6647 bp Coordinate of oriT [Strand]   6273..6355 [-]
Host baterium   Salmonella enterica subsp. enterica serovar Heidelberg strain SH14-028

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -