Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100819
Name   oriT_pESBL931 in_silico
Organism   Salmonella enterica subsp. enterica serovar Typhimurium strain ST931R
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP016389 (40785..41334 [-], 550 nt)
oriT length   550 nt
IRs (inverted repeats)      243..255, 257..269  (TATTTAATAATTC..GAATTATTAAATA)
 311..318, 321..328  (GCAAAAAC..GTTTTTGC)
Location of nic site      337..338
Conserved sequence flanking the
  nic site    		  GTAGTGTGT|GG
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 550 nt

>oriT_pESBL931
TTACTCTGGCCATAAGATAAAACCTTTCATTATTAAGCAATGAACTTTTCACTATAAATATGCATATAGTGTTTACAAGTAAGAAAGACACTCCTAGCAGCGCCTCTAGGATCATCCTATAAAAAAATGCGATCCGGCGCTAGGGGCGTCCCTAATATATATCAATGTTTTTCATGAAAATTGTCAGTACTGATCCTAATAAGAGTCGCTATAGGGTCGTAACAGGATCGCCAACGACTCTCTATTTAATAATTCAGAATTATTAAATATAAATAGCGTTTGTTAATTACATGATTTAAAACGTAAATCAGCAAAAACTTGTTTTTGCGTAGTGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCTTTTTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAGGTGGAAAAATGAAAAAATGGATGTTAGCAATCTGCCTGATGTTTATAAATGGGATCTGCGAAGCCGCCGATTGCTTTGATCTTGCAGGTCGGGATTACAAAATAGACCCGGATTTACTGAGAGCGATATC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   891 GenBank   WP_000948354
Name   TraI_pESBL931 insolico UniProt ID   _
Length   1756 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 1756 a.a.        Molecular weight: 191683.22 Da        Isoelectric Point: 5.6912

>WP_000948354.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]
MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQDPQLHTHVVVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEA
LGYETEVVGKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLQEGMVFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYDRLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVADSPFTALKL
ENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETSLETAISLQKTGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPASERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVVIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEA
QQKAMLKGETFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGLITLEDAEGNTRLISPREAVAEGVT
LYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVL
EPKPDREVMNAQRLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDSSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGKTEQAVREIAGQERDRADITEREAALPESVLRESQREQEAVREVARENLLQERLQQIERDMVRDLQKE
KTLGGD

  Protein domains


Predicted by InterproScan.

DNA helicase TraI (TraI_C)

(1434-1557)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(633-712)

TrwC relaxase (TrwC)

(10-283)

AAA domain (AAA_30)

(968-1156)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(575-626)


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 40397..64007

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BBC45_RS24570 (BBC45_00210) 36369..36803 + 435 WP_000845953 conjugation system SOS inhibitor PsiB -
BBC45_RS24575 (BBC45_00215) 36800..37519 + 720 WP_001276217 plasmid SOS inhibition protein A -
BBC45_RS28410 37741..37890 + 150 Protein_48 plasmid maintenance protein Mok -
BBC45_RS24580 (BBC45_00220) 37832..37957 + 126 WP_001372321 type I toxin-antitoxin system Hok family toxin -
BBC45_RS28100 (BBC45_00225) 38276..38572 - 297 Protein_50 hypothetical protein -
BBC45_RS24590 (BBC45_00230) 38872..39168 + 297 WP_001272251 hypothetical protein -
BBC45_RS24595 (BBC45_00235) 39279..40100 + 822 WP_001234445 DUF932 domain-containing protein -
BBC45_RS24600 (BBC45_00240) 40397..40999 - 603 WP_000243713 transglycosylase SLT domain-containing protein virB1
BBC45_RS24605 (BBC45_00245) 41322..41705 + 384 WP_001354030 conjugal transfer relaxosome DNA-binding protein TraM -
BBC45_RS24610 (BBC45_00250) 41899..42570 + 672 WP_000283561 conjugal transfer transcriptional regulator TraJ -
BBC45_RS24615 42707..42934 + 228 WP_000089263 conjugal transfer relaxosome protein TraY -
BBC45_RS24620 (BBC45_00255) 42967..43326 + 360 WP_001098992 type IV conjugative transfer system pilin TraA -
BBC45_RS24625 (BBC45_00260) 43341..43652 + 312 WP_000012113 type IV conjugative transfer system protein TraL traL
BBC45_RS24630 (BBC45_00265) 43674..44240 + 567 WP_000399780 type IV conjugative transfer system protein TraE traE
BBC45_RS24635 (BBC45_00270) 44227..44955 + 729 WP_001230772 type-F conjugative transfer system secretin TraK traK
BBC45_RS24640 (BBC45_00275) 44955..46406 + 1452 WP_000146675 F-type conjugal transfer pilus assembly protein TraB traB
BBC45_RS24645 (BBC45_00280) 46396..46962 + 567 WP_000896599 conjugal transfer pilus-stabilizing protein TraP -
BBC45_RS24650 (BBC45_00285) 46949..47269 + 321 WP_001057307 conjugal transfer protein TrbD -
BBC45_RS24655 (BBC45_00290) 47266..47781 + 516 WP_000809881 type IV conjugative transfer system lipoprotein TraV traV
BBC45_RS27035 47916..48137 + 222 WP_001278683 conjugal transfer protein TraR -
BBC45_RS24660 (BBC45_00295) 48130..48606 + 477 WP_000549587 hypothetical protein -
BBC45_RS24665 (BBC45_00300) 48686..48904 + 219 WP_000556745 hypothetical protein -
BBC45_RS24670 (BBC45_00305) 48932..49279 + 348 WP_000836682 hypothetical protein -
BBC45_RS24675 (BBC45_00310) 49405..52035 + 2631 WP_000069777 type IV secretion system protein TraC virb4
BBC45_RS24680 (BBC45_00315) 52032..52418 + 387 WP_000214084 type-F conjugative transfer system protein TrbI -
BBC45_RS24685 (BBC45_00320) 52415..53047 + 633 WP_001203728 type-F conjugative transfer system protein TraW traW
BBC45_RS24690 (BBC45_00325) 53044..54036 + 993 WP_000830838 conjugal transfer pilus assembly protein TraU traU
BBC45_RS24695 (BBC45_00330) 54066..54371 + 306 WP_000224416 hypothetical protein -
BBC45_RS24700 (BBC45_00335) 54380..55018 + 639 WP_001080256 type-F conjugative transfer system pilin assembly protein TrbC trbC
BBC45_RS24705 (BBC45_00340) 55015..56865 + 1851 WP_000821856 type-F conjugative transfer system mating-pair stabilization protein TraN traN
BBC45_RS24710 (BBC45_00345) 56892..57149 + 258 WP_000864353 conjugal transfer protein TrbE -
BBC45_RS24715 (BBC45_00350) 57142..57885 + 744 WP_001030371 type-F conjugative transfer system pilin assembly protein TraF traF
BBC45_RS27040 57899..58243 + 345 WP_000556796 conjugal transfer protein TrbA -
BBC45_RS24720 (BBC45_00355) 58362..58646 + 285 WP_000624194 type-F conjugative transfer system pilin chaperone TraQ -
BBC45_RS24725 (BBC45_00360) 58633..59178 + 546 WP_000059831 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
BBC45_RS24730 (BBC45_00365) 59108..59455 + 348 WP_001309242 P-type conjugative transfer protein TrbJ -
BBC45_RS24735 (BBC45_00370) 59436..59828 + 393 WP_000660699 F-type conjugal transfer protein TrbF -
BBC45_RS24740 (BBC45_00375) 59815..61188 + 1374 WP_000944331 conjugal transfer pilus assembly protein TraH traH
BBC45_RS24745 (BBC45_00380) 61185..64007 + 2823 WP_001007039 conjugal transfer mating-pair stabilization protein TraG traG
BBC45_RS27045 64023..64508 + 486 WP_000605870 hypothetical protein -
BBC45_RS24750 (BBC45_00385) 64557..65288 + 732 WP_000782451 conjugal transfer complement resistance protein TraT -
BBC45_RS24755 (BBC45_00390) 65491..66228 + 738 WP_000199905 hypothetical protein -

Host bacterium

ID   1279 GenBank   NZ_CP016389
Plasmid name   pESBL931 Incompatibility group   IncFII
Plasmid size   68117 bp Coordinate of oriT [Strand]   40785..41334 [-]
Host baterium   Salmonella enterica subsp. enterica serovar Typhimurium strain ST931R

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -