Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100818 |
| Name | oriT_p931IncI1  |
| Organism | Salmonella enterica subsp. enterica serovar Typhimurium strain ST931R |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP016387 (78855..78964 [+], 110 nt) |
| oriT length | 110 nt |
| IRs (inverted repeats) | IR1: 24..31, 35..42 (GTCGGGGC..GCCCTGAC) IR2: 49..65, 72..88 (GTAATTGTAATAGCGTC..GACGGTATTACAATTAC) |
| Location of nic site | 96..97 |
| Conserved sequence flanking the nic site |
CATCCTG|T |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 110 nt
>oriT_p931IncI1
TCACTTCAGGCTCCTTACGGGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTACACATCCTGTCCCGTTTTTCAGG
TCACTTCAGGCTCCTTACGGGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTACACATCCTGTCCCGTTTTTCAGG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 890 | GenBank | WP_039005629 |
| Name | NikB_p931IncI1 |
UniProt ID | _ |
| Length | 899 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 899 a.a. Molecular weight: 103950.51 Da Isoelectric Point: 7.4195
>WP_039005629.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRNRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIAQQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVR
HTLKSLGLGEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFHEYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNRKGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPENRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGELVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFKRDPRMGFEPEGNDGQFNQVFAEMVAW
YNVTGFSEHGNYVITRPDVDQHREVSERGYRNYMDSNTYRDGAQPVQDSENRWEPPTPV
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRNRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIAQQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVR
HTLKSLGLGEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFHEYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNRKGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPENRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGELVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFKRDPRMGFEPEGNDGQFNQVFAEMVAW
YNVTGFSEHGNYVITRPDVDQHREVSERGYRNYMDSNTYRDGAQPVQDSENRWEPPTPV
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 32312..67919
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BBC45_RS26765 | 27374..28441 | + | 1068 | WP_001360287 | type IV pilus biogenesis lipoprotein PilL | - |
| BBC45_RS23690 (BBC45_00575) | 28441..28878 | + | 438 | WP_000539807 | type IV pilus biogenesis protein PilM | - |
| BBC45_RS23695 (BBC45_00580) | 28892..30574 | + | 1683 | WP_000748143 | PilN family type IVB pilus formation outer membrane protein | - |
| BBC45_RS23700 (BBC45_00585) | 30567..31862 | + | 1296 | WP_000752780 | type 4b pilus protein PilO2 | - |
| BBC45_RS23705 (BBC45_00590) | 31849..32301 | + | 453 | WP_001247337 | type IV pilus biogenesis protein PilP | - |
| BBC45_RS23710 (BBC45_00595) | 32312..33865 | + | 1554 | WP_000362206 | ATPase, T2SS/T4P/T4SS family | virB11 |
| BBC45_RS23715 (BBC45_00600) | 33878..34963 | + | 1086 | WP_001208802 | type II secretion system F family protein | - |
| BBC45_RS23720 (BBC45_00605) | 34980..35594 | + | 615 | WP_000908226 | type 4 pilus major pilin | - |
| BBC45_RS23725 (BBC45_00610) | 35604..36164 | + | 561 | WP_000014005 | lytic transglycosylase domain-containing protein | virB1 |
| BBC45_RS23730 (BBC45_00615) | 36149..36805 | + | 657 | WP_001193549 | A24 family peptidase | - |
| BBC45_RS23735 (BBC45_00620) | 36805..38097 | + | 1293 | WP_001350014 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| BBC45_RS27545 | 38154..38279 | - | 126 | Protein_49 | transposase | - |
| BBC45_RS23740 (BBC45_00625) | 38487..39311 | + | 825 | WP_069601577 | conjugal transfer protein TraE | traE |
| BBC45_RS23745 (BBC45_00630) | 39397..40599 | + | 1203 | WP_000976348 | conjugal transfer protein TraF | - |
| BBC45_RS23750 (BBC45_00635) | 40659..41243 | + | 585 | WP_000977522 | histidine phosphatase family protein | - |
| BBC45_RS23755 (BBC45_00640) | 41638..42096 | + | 459 | WP_022630640 | IncI1-type conjugal transfer lipoprotein TraH | - |
| BBC45_RS23760 (BBC45_00645) | 42093..42911 | + | 819 | WP_000866663 | IncI1-type conjugal transfer lipoprotein TraI | traI |
| BBC45_RS23765 (BBC45_00650) | 42908..44056 | + | 1149 | WP_001024972 | plasmid transfer ATPase TraJ | virB11 |
| BBC45_RS26770 | 44053..44343 | + | 291 | WP_001299214 | hypothetical protein | traK |
| BBC45_RS23770 (BBC45_00655) | 44358..44909 | + | 552 | WP_022630639 | phospholipase D family protein | - |
| BBC45_RS23775 (BBC45_00660) | 44999..48766 | + | 3768 | WP_022630638 | LPD7 domain-containing protein | - |
| BBC45_RS23780 (BBC45_00665) | 48784..49131 | + | 348 | WP_001055900 | conjugal transfer protein | traL |
| BBC45_RS23785 (BBC45_00670) | 49128..49820 | + | 693 | WP_000138552 | DotI/IcmL family type IV secretion protein | traM |
| BBC45_RS23790 (BBC45_00675) | 49831..50814 | + | 984 | WP_022630637 | IncI1-type conjugal transfer protein TraN | traN |
| BBC45_RS23795 (BBC45_00680) | 50817..52106 | + | 1290 | WP_001271994 | conjugal transfer protein TraO | traO |
| BBC45_RS23800 (BBC45_00685) | 52106..52810 | + | 705 | WP_000801920 | IncI1-type conjugal transfer protein TraP | traP |
| BBC45_RS23805 (BBC45_00690) | 52810..53337 | + | 528 | WP_001055569 | conjugal transfer protein TraQ | traQ |
| BBC45_RS23810 (BBC45_00695) | 53388..53792 | + | 405 | WP_001422824 | IncI1-type conjugal transfer protein TraR | traR |
| BBC45_RS23815 (BBC45_00700) | 53856..54044 | + | 189 | WP_001277253 | putative conjugal transfer protein TraS | - |
| BBC45_RS23820 (BBC45_00705) | 54028..54828 | + | 801 | WP_022630636 | IncI1-type conjugal transfer protein TraT | traT |
| BBC45_RS23825 (BBC45_00710) | 54918..57962 | + | 3045 | WP_022630635 | IncI1-type conjugal transfer protein TraU | traU |
| BBC45_RS26775 | 57962..58576 | + | 615 | WP_000337399 | IncI1-type conjugal transfer protein TraV | traV |
| BBC45_RS23830 (BBC45_00715) | 58543..59745 | + | 1203 | WP_001189156 | IncI1-type conjugal transfer protein TraW | traW |
| BBC45_RS23835 (BBC45_00720) | 59774..60358 | + | 585 | WP_001037987 | IncI1-type conjugal transfer protein TraX | - |
| BBC45_RS23840 (BBC45_00725) | 60455..62623 | + | 2169 | WP_000698357 | DotA/TraY family protein | traY |
| BBC45_RS23845 (BBC45_00730) | 62697..63347 | + | 651 | WP_001178506 | plasmid IncI1-type surface exclusion protein ExcA | - |
| BBC45_RS23850 (BBC45_00735) | 63419..63628 | - | 210 | WP_000062603 | HEAT repeat domain-containing protein | - |
| BBC45_RS28045 | 64020..64196 | + | 177 | WP_001054897 | hypothetical protein | - |
| BBC45_RS28535 | 64261..64356 | - | 96 | WP_001303310 | DinQ-like type I toxin DqlB | - |
| BBC45_RS23855 (BBC45_00740) | 64855..65106 | + | 252 | WP_001291964 | hypothetical protein | - |
| BBC45_RS23860 (BBC45_00745) | 65178..65330 | - | 153 | WP_001331364 | Hok/Gef family protein | - |
| BBC45_RS23865 (BBC45_00750) | 65622..66830 | + | 1209 | WP_016245570 | IncI1-type conjugal transfer protein TrbA | trbA |
| BBC45_RS23870 (BBC45_00755) | 66849..67919 | + | 1071 | WP_000151583 | IncI1-type conjugal transfer protein TrbB | trbB |
| BBC45_RS23875 (BBC45_00760) | 67912..70203 | + | 2292 | WP_024178322 | F-type conjugative transfer protein TrbC | - |
| BBC45_RS28385 | 71261..71623 | + | 363 | WP_085004988 | CPBP family intramembrane glutamic endopeptidase | - |
Host bacterium
| ID | 1278 | GenBank | NZ_CP016387 |
| Plasmid name | p931IncI1 | Incompatibility group | IncI1 |
| Plasmid size | 93202 bp | Coordinate of oriT [Strand] | 78855..78964 [+] |
| Host baterium | Salmonella enterica subsp. enterica serovar Typhimurium strain ST931R |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |