Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100817
Name   oriT_pC06114_6 in_silico
Organism   Escherichia coli isolate Co6114
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP016040 (2690..2772 [-], 83 nt)
oriT length   83 nt
IRs (inverted repeats)      3..12, 16..25  (GTGTCGGGGC..GCCCTGACCC)
Location of nic site      56..57
Conserved sequence flanking the
  nic site    		  CTGG|CTTA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 83 nt

>oriT_pC06114_6
GGGTGTCGGGGCGCAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGTGGCATCAGTGCGGATT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   889 GenBank   WP_063269975
Name   Nuclease_pC06114_6 insolico UniProt ID   _
Length   529 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 529 a.a.        Molecular weight: 59962.63 Da        Isoelectric Point: 9.9167

>WP_063269975.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacteriaceae]
MIVKFHARGKGGGSGPVDYLLGRERNREGATVLRGNPEEIRELIDATPFSKKYTSGVLSFAEKELPPGER
ERVMTSFERVLMPGLEKDQYSILWVEHQDKGRLELNFVIPNMELQSGKRLQPYYDRADRPRINAWQTLVN
HHYGLRDPNAPENRRTLVTPNNLPKAKQEAAEAITRGLEALYRAGAIKTRQDVTEALTAAGFEVVRTTRS
SISIADPEGGRNLRLKGALYEQSFTNGNRLREETERASRFYREHAEQRVQEARRVCRQGTELKQAENQRR
YQRTYNIRETATGYAEHHREHAAQHHRGRTVPAAEKPALAGDQRAGTVSGADRAQRHGRHFPGVLGADSV
AGKQDCEKYLDHQRAGKHAGETRGKNLGGDVRQWREREISGAAGRGERGKHLDGGRQERREAGQGVNEYD
GTGRAFAERLRAFATGLYAAAERVGERLRRIAEDARGYATEQRDTERACRELESAGAAISRTSRPLESVI
YHEQQIRDEQERQAQELARRWHEEQEPEARIWRGPSMGM

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(57-240)


  Protein structure



No available structure.




Host bacterium

ID   1277 GenBank   NZ_CP016040
Plasmid name   pC06114_6 Incompatibility group   ColRNAI
Plasmid size   4634 bp Coordinate of oriT [Strand]   2690..2772 [-]
Host baterium   Escherichia coli isolate Co6114

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -