Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100815
Name   oriT_pC06114_2 in_silico
Organism   Escherichia coli isolate Co6114
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP016036 (81303..81853 [+], 551 nt)
oriT length   551 nt
IRs (inverted repeats)      IR1: 62..70, 71..79  (AACCCTTTC..GACAGGGTT)
  IR2: 143..150, 154..161  (TGGCCTGC..GGAGGCCA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 551 nt

>oriT_pC06114_2
GAGCAGAGCTATGTGTGACAAGAAGTATAGAAATTACGAGGTAGCCATCATGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAAGGCTAATCAGGAGCCTGTGATTTACCCGATCATTGAAGAAGCTCTGCATCGCTACAGCCAGCTCGTGTTTCATGAGCAGAGAGAGAAATATGAAGACCCGGCCAGAATTGGGGCATTTCTGGAAACCCTGATCACCGAAACCTGCCGGGCGTTGGAAGTGCAAATTGTCGATAGTGGCGGTGATTCATGGTCTGTCGATTCAGGAGAGTCGTTCTCACTGTGGCTTTCTTCCCATCCAGGAGAACTATCCATTAACCCGCAGCCCCATGAGGATGAGACCTCTTTGCGTGGCTTGCTGTATGAGCTCATCACCTGTGAGAGCGTGAAAACTGTTTTAAGGAGAACCGACT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   887 GenBank   WP_001326173
Name   TraI_pC06114_2 insolico UniProt ID   _
Length   990 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 990 a.a.        Molecular weight: 109356.90 Da        Isoelectric Point: 4.9395

>WP_001326173.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 79437..88527

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A8V37_RS25355 (A8V37_25355) 75468..75701 - 234 WP_001191890 hypothetical protein -
A8V37_RS25360 (A8V37_25360) 75683..76300 - 618 WP_001249395 hypothetical protein -
A8V37_RS25365 (A8V37_25365) 76468..79440 + 2973 WP_001326173 MobH family relaxase -
A8V37_RS25370 (A8V37_25370) 79437..81302 + 1866 WP_000178857 conjugative transfer system coupling protein TraD virb4
A8V37_RS25375 (A8V37_25375) 81313..81897 + 585 WP_000332868 hypothetical protein -
A8V37_RS25380 (A8V37_25380) 81854..82483 + 630 WP_000743449 DUF4400 domain-containing protein tfc7
A8V37_RS25385 (A8V37_25385) 82493..82939 + 447 WP_000122507 hypothetical protein -
A8V37_RS25390 (A8V37_25390) 82949..83326 + 378 WP_000869297 hypothetical protein -
A8V37_RS25395 (A8V37_25395) 83326..83988 + 663 WP_001231464 hypothetical protein -
A8V37_RS25400 (A8V37_25400) 84312..84689 + 378 WP_001326174 hypothetical protein -
A8V37_RS25405 (A8V37_25405) 84834..85115 + 282 WP_000805625 type IV conjugative transfer system protein TraL traL
A8V37_RS25410 (A8V37_25410) 85112..85738 + 627 WP_001049717 TraE/TraK family type IV conjugative transfer system protein traE
A8V37_RS25415 (A8V37_25415) 85722..86639 + 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
A8V37_RS25420 (A8V37_25420) 86636..87952 + 1317 WP_000595749 TraB/VirB10 family protein traB
A8V37_RS25425 (A8V37_25425) 87949..88527 + 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
A8V37_RS25430 (A8V37_25430) 88531..88923 + 393 WP_000479535 TraA family conjugative transfer protein -
A8V37_RS25435 (A8V37_25435) 89124..91901 + 2778 WP_077791902 Ig-like domain-containing protein -
A8V37_RS29145 91935..92009 - 75 Protein_122 IS6 family transposase -

Host bacterium

ID   1275 GenBank   NZ_CP016036
Plasmid name   pC06114_2 Incompatibility group   IncA/C2
Plasmid size   92009 bp Coordinate of oriT [Strand]   81303..81853 [+]
Host baterium   Escherichia coli isolate Co6114

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -