Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100813 |
| Name | oriT_pNGF1_pCol_let_like  |
| Organism | Escherichia coli strain NGF1 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP016009 (1838..1920 [+], 83 nt) |
| oriT length | 83 nt |
| IRs (inverted repeats) | 3..12, 16..25 (GTGTCGGGGC..GCCCTGACCC) |
| Location of nic site | 56..57 |
| Conserved sequence flanking the nic site |
CTGG|CTTA |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 83 nt
>oriT_pNGF1_pCol_let_like
GGGTGTCGGGGCGCAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGACATCAGTGCGGATT
GGGTGTCGGGGCGCAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGACATCAGTGCGGATT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 885 | GenBank | WP_032286519 |
| Name | MobC_pNGF1_pCol_let_like |
UniProt ID | _ |
| Length | 107 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 107 a.a. Molecular weight: 11903.77 Da Isoelectric Point: 10.8973
>WP_032286519.1 plasmid mobilization relaxosome protein MobC [Escherichia coli]
MLTIRVTDDEHARLLERCEGKQMAVWMRRVCLGEPVARSGKLPTLAPPLLRQLAAIGNNLNQTARKVNSG
QWSSGDRVQVVAALMAIGDELRRLRLAVREQGTRDDS
MLTIRVTDDEHARLLERCEGKQMAVWMRRVCLGEPVARSGKLPTLAPPLLRQLAAIGNNLNQTARKVNSG
QWSSGDRVQVVAALMAIGDELRRLRLAVREQGTRDDS
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Host bacterium
| ID | 1273 | GenBank | NZ_CP016009 |
| Plasmid name | pNGF1_pCol_let_like | Incompatibility group | ColRNAI |
| Plasmid size | 8556 bp | Coordinate of oriT [Strand] | 1838..1920 [+] |
| Host baterium | Escherichia coli strain NGF1 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |