Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100811
Name   oriT_pSLy5 in_silico
Organism   Escherichia coli strain 210205630 isolate 210205630
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP015917 (8024..8197 [-], 174 nt)
oriT length   174 nt
IRs (inverted repeats)      2..11, 15..24  (GTGTCGGGGC..GCCCTGAACC)
Location of nic site      40..41
Conserved sequence flanking the
  nic site    		  CTAG|CGGA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 174 nt

>oriT_pSLy5
GGTGTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTATTATGTAGGCACGGGTGCGGGAATCAGTGAAGTGCTTCATGTGGCAGGAGGAAAATGCAGCAGCAGAGCGTAAGCGGAATATATGAGACAGGAGATATTCCGCTTCCTCGCTCAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   883 GenBank   WP_000635804
Name   Nuclease_pSLy5 insolico UniProt ID   _
Length   517 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 517 a.a.        Molecular weight: 57794.27 Da        Isoelectric Point: 6.0811

>WP_000635804.1 MULTISPECIES: nuclease [Enterobacteriaceae]
MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSFAEKELPPGGR
EKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLVN
HHYGLHDPNAPENRRTLTLPDNLPETKQALAEGVTRGIDALYHAGEIKGRQDVIQALTEAGLEVVRVTRS
SISIADPNGGKNIRLKGAFYEQSFADGRGVREKAERESRIYRENAEQRVQEARRICKRGCDIKRDENQRR
YSPVHSLDRGIAGKTPGRGERGDDAAQEGRVKAGREYGHDVTGDSLSPVYREWRDALVSWREDTGEPGRN
QEAGRDIAETEREDMGRGVCAGREQEIPCPSVREISGGDSLSGERVGTSEGVTQSDRAGNTFAERLRAAA
TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQRELEIREERLIQE
REHVLSLERERQPEIQERTLDGPSLGW

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(57-237)


  Protein structure



No available structure.




Host bacterium

ID   1271 GenBank   NZ_CP015917
Plasmid name   pSLy5 Incompatibility group   Col440I
Plasmid size   8972 bp Coordinate of oriT [Strand]   8024..8197 [-]
Host baterium   Escherichia coli strain 210205630 isolate 210205630

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -