Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100807
Name   oriT_pMS6198A in_silico
Organism   Escherichia coli strain MS6198
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP015835 (47479..48029 [+], 551 nt)
oriT length   551 nt
IRs (inverted repeats)      IR1: 62..70, 71..79  (AACCCTTTC..GACAGGGTT)
  IR2: 143..150, 154..161  (TGGCCTGC..GGAGGCCA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 551 nt

>oriT_pMS6198A
GAGCAGAGCTATGTGTGACAAGAAGTATAGAAATTACGAGGTAGCCATCATGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAAGGCTAATCAGGAGCCTGTGATTTACCCGATCATTGAAGAAGCTCTGCATCGCTACAGCCAGCTCGTGTTTCATGAGCAGAGAGAGAAATATGAAGACCCGGCCAGAATTGGGGCATTTCTGGAAACCCTGATCACCGAAACCTGCCGGGCGTTGGAAGTGCAAATTGTCGATAGTGGCGGTGATTCATGGTCTGTCGATTCAGGAGAGTCGTTCTCACTGTGGCTTTCTTCCCATCCAGGAGAACTATCCATTAACCCGCAGCCCCATGAGGATGAGACCTCTTTGCGTGGCTTGCTGTATGAGCTCATCACCTGTGAGAGCGTGAAAACTGTTTTAAGGAGAACCGACT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   879 GenBank   WP_001326173
Name   TraI_pMS6198A insolico UniProt ID   C4NVS7
Length   990 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 990 a.a.        Molecular weight: 109356.90 Da        Isoelectric Point: 4.9395

>WP_001326173.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure


Source ID Structure
AlphaFold DB C4NVS7


T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 45613..54703

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MS6198_RS25855 (MS6198_A069) 41644..41877 - 234 WP_001191890 hypothetical protein -
MS6198_RS25860 (MS6198_A070) 41859..42476 - 618 WP_001249395 hypothetical protein -
MS6198_RS25865 (MS6198_A071) 42644..45616 + 2973 WP_001326173 MobH family relaxase -
MS6198_RS25870 (MS6198_A072) 45613..47478 + 1866 WP_000178857 conjugative transfer system coupling protein TraD virb4
MS6198_RS25875 (MS6198_A073) 47528..48073 + 546 WP_000228720 hypothetical protein -
MS6198_RS25880 (MS6198_A074) 48030..48659 + 630 WP_000743449 DUF4400 domain-containing protein tfc7
MS6198_RS25885 (MS6198_A075) 48669..49115 + 447 WP_000122507 hypothetical protein -
MS6198_RS25890 (MS6198_A076) 49125..49502 + 378 WP_000869297 hypothetical protein -
MS6198_RS25895 (MS6198_A077) 49502..50164 + 663 WP_001231464 hypothetical protein -
MS6198_RS31575 (MS6198_A078) 50345..50488 + 144 WP_001275801 hypothetical protein -
MS6198_RS25900 (MS6198_A079) 50500..50865 + 366 WP_001052530 hypothetical protein -
MS6198_RS25905 (MS6198_A081) 51010..51291 + 282 WP_000805625 type IV conjugative transfer system protein TraL traL
MS6198_RS25910 (MS6198_A082) 51288..51914 + 627 WP_001049717 TraE/TraK family type IV conjugative transfer system protein traE
MS6198_RS25915 (MS6198_A083) 51898..52815 + 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
MS6198_RS25920 (MS6198_A084) 52815..54128 + 1314 WP_024131605 TraB/VirB10 family protein traB
MS6198_RS25925 (MS6198_A085) 54125..54703 + 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
MS6198_RS25930 (MS6198_A086) 54707..55099 + 393 WP_000479535 TraA family conjugative transfer protein -
MS6198_RS25935 (MS6198_A087) 55384..56646 + 1263 WP_000608644 IS1380-like element ISEcp1 family transposase -
MS6198_RS25945 (MS6198_A089) 56970..58115 + 1146 WP_015058212 class C beta-lactamase CMY-6 -
MS6198_RS25950 (MS6198_A090) 58209..58742 + 534 WP_001221666 lipocalin family protein -
MS6198_RS25955 (MS6198_A091) 58739..59056 - 318 WP_000118520 quaternary ammonium compound efflux SMR transporter SugE -
MS6198_RS32380 (MS6198_A093) 59313..59678 + 366 Protein_87 LuxR family transcriptional regulator -

Host bacterium

ID   1267 GenBank   NZ_CP015835
Plasmid name   pMS6198A Incompatibility group   IncA/C2
Plasmid size   137565 bp Coordinate of oriT [Strand]   47479..48029 [+]
Host baterium   Escherichia coli strain MS6198

Cargo genes

Drug resistance gene   blaCMY-6, aac(6')-Ib, qacE, sul1, rmtC, blaNDM-1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -