Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100797 |
| Name | oriT_pEC732_IMP14  |
| Organism | Escherichia coli strain Ecol_732 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP015139 (88649..89199 [-], 551 nt) |
| oriT length | 551 nt |
| IRs (inverted repeats) | IR1: 62..70, 71..79 (AACCCTTTC..GACAGGGTT) IR2: 143..150, 154..161 (TGGCCTGC..GGAGGCCA) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 551 nt
>oriT_pEC732_IMP14
GAGCAGAGCTATGTGTGACAAGAAGTATAGAAATTACGAGGTAGCCATCATGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAAGGCTAATCAGGAGCCTGTGATTTACCCGATCATTGAAGAAGCTCTGCATCGCTACAGCCAGCTCGTGTTTCATGAGCAGAGAGAGAAATATGAAGACCCGGCCAGAATTGGGGCATTTCTGGAAACCCTGATCACCGAAACCTGCCGGGCGTTGGAAGTGCAAATTGTCGATAGTGGCGGTGATTCATGGTCTGTCGATTCAGGAGAGTCGTTCTCACTGTGGCTTTCTTCCCATCCAGGAGAACTATCCATTAACCCGCAGCCCCATGAGGATGAGACCTCTTTGCGTGGCTTGCTGTATGAGCTCATCACCTGTGAGAGCGTGAAAACTGTTTTAAGGAGAACCGACT
GAGCAGAGCTATGTGTGACAAGAAGTATAGAAATTACGAGGTAGCCATCATGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAAGGCTAATCAGGAGCCTGTGATTTACCCGATCATTGAAGAAGCTCTGCATCGCTACAGCCAGCTCGTGTTTCATGAGCAGAGAGAGAAATATGAAGACCCGGCCAGAATTGGGGCATTTCTGGAAACCCTGATCACCGAAACCTGCCGGGCGTTGGAAGTGCAAATTGTCGATAGTGGCGGTGATTCATGGTCTGTCGATTCAGGAGAGTCGTTCTCACTGTGGCTTTCTTCCCATCCAGGAGAACTATCCATTAACCCGCAGCCCCATGAGGATGAGACCTCTTTGCGTGGCTTGCTGTATGAGCTCATCACCTGTGAGAGCGTGAAAACTGTTTTAAGGAGAACCGACT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 869 | GenBank | WP_000130000 |
| Name | Relaxase_pEC732_IMP14 |
UniProt ID | _ |
| Length | 101 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 101 a.a. Molecular weight: 11477.11 Da Isoelectric Point: 7.5204
>WP_000130000.1 MULTISPECIES: PadR family transcriptional regulator [Pseudomonadota]
MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGTVYPMLHGLEKKGYLTSRHERTGRRER
RVYDITEQGRTALADAKTKVKELFGELVEGG
MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGTVYPMLHGLEKKGYLTSRHERTGRRER
RVYDITEQGRTALADAKTKVKELFGELVEGG
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 65922..91065
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| A4X18_RS25320 (A4X18_25305) | 61048..61956 | - | 909 | WP_000739139 | hypothetical protein | - |
| A4X18_RS25325 (A4X18_25310) | 61967..62935 | - | 969 | WP_000085160 | AAA family ATPase | - |
| A4X18_RS25330 (A4X18_25315) | 63155..63340 | - | 186 | WP_001186917 | hypothetical protein | - |
| A4X18_RS25335 (A4X18_25320) | 63644..63964 | - | 321 | WP_000547566 | hypothetical protein | - |
| A4X18_RS25340 (A4X18_25325) | 64259..64900 | + | 642 | WP_000796664 | hypothetical protein | - |
| A4X18_RS25345 (A4X18_25330) | 65023..65883 | + | 861 | WP_000709517 | hypothetical protein | - |
| A4X18_RS25350 (A4X18_25335) | 65922..68717 | - | 2796 | WP_001256484 | conjugal transfer mating pair stabilization protein TraN | traN |
| A4X18_RS25355 (A4X18_25340) | 68833..69837 | - | 1005 | WP_005507569 | TraU family protein | traU |
| A4X18_RS25360 (A4X18_25345) | 69837..70508 | - | 672 | WP_000575345 | EAL domain-containing protein | - |
| A4X18_RS25365 (A4X18_25350) | 70505..71770 | - | 1266 | WP_001447719 | TrbC family F-type conjugative pilus assembly protein | traW |
| A4X18_RS25370 (A4X18_25355) | 71733..72263 | - | 531 | WP_001010740 | S26 family signal peptidase | - |
| A4X18_RS25375 (A4X18_25360) | 72260..72577 | - | 318 | WP_000351984 | hypothetical protein | - |
| A4X18_RS25380 (A4X18_25365) | 72592..75039 | - | 2448 | WP_000637384 | type IV secretion system protein TraC | virb4 |
| A4X18_RS25385 (A4X18_25370) | 75036..75743 | - | 708 | WP_001259346 | DsbC family protein | - |
| A4X18_RS25390 (A4X18_25375) | 75892..81378 | - | 5487 | WP_000606835 | Ig-like domain-containing protein | - |
| A4X18_RS25395 (A4X18_25380) | 81579..81971 | - | 393 | WP_000479535 | TraA family conjugative transfer protein | - |
| A4X18_RS25400 (A4X18_25385) | 81975..82553 | - | 579 | WP_000793435 | type IV conjugative transfer system lipoprotein TraV | traV |
| A4X18_RS25405 (A4X18_25390) | 82550..83863 | - | 1314 | WP_024131605 | TraB/VirB10 family protein | traB |
| A4X18_RS25410 (A4X18_25395) | 83863..84780 | - | 918 | WP_000794249 | type-F conjugative transfer system secretin TraK | traK |
| A4X18_RS25415 (A4X18_25400) | 84764..85390 | - | 627 | WP_001049717 | TraE/TraK family type IV conjugative transfer system protein | traE |
| A4X18_RS25420 (A4X18_25405) | 85387..85668 | - | 282 | WP_000805625 | type IV conjugative transfer system protein TraL | traL |
| A4X18_RS25425 (A4X18_25410) | 85813..86178 | - | 366 | WP_001052530 | hypothetical protein | - |
| A4X18_RS31515 | 86190..86333 | - | 144 | WP_001275801 | hypothetical protein | - |
| A4X18_RS25430 (A4X18_25415) | 86514..87176 | - | 663 | WP_001231464 | hypothetical protein | - |
| A4X18_RS25435 (A4X18_25420) | 87176..87553 | - | 378 | WP_000869297 | hypothetical protein | - |
| A4X18_RS25440 (A4X18_25425) | 87563..88009 | - | 447 | WP_000122507 | hypothetical protein | - |
| A4X18_RS25445 (A4X18_25430) | 88019..88648 | - | 630 | WP_000743449 | DUF4400 domain-containing protein | tfc7 |
| A4X18_RS25450 (A4X18_25435) | 88605..89150 | - | 546 | WP_000228720 | hypothetical protein | - |
| A4X18_RS25455 (A4X18_25440) | 89200..91065 | - | 1866 | WP_000178857 | conjugative transfer system coupling protein TraD | virb4 |
| A4X18_RS25460 (A4X18_25445) | 91062..94034 | - | 2973 | WP_001326173 | MobH family relaxase | - |
| A4X18_RS25465 (A4X18_25450) | 94202..94819 | + | 618 | WP_001249395 | hypothetical protein | - |
| A4X18_RS25470 (A4X18_25455) | 94801..95034 | + | 234 | WP_001191890 | hypothetical protein | - |
Host bacterium
| ID | 1257 | GenBank | NZ_CP015139 |
| Plasmid name | pEC732_IMP14 | Incompatibility group | IncA/C2 |
| Plasmid size | 186826 bp | Coordinate of oriT [Strand] | 88649..89199 [-] |
| Host baterium | Escherichia coli strain Ecol_732 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | mercury resistance |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |