Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100784 |
| Name | oriT_pKQPS2  |
| Organism | Klebsiella quasipneumoniae strain ATCC 700603 isolate K6 |
| Sequence Completeness | core |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP014698 (38701..38805 [+], 105 nt) |
| oriT length | 105 nt |
| IRs (inverted repeats) | 18..35, 42..59 (GTTTTTGGTACACCGCCG..CGGCAGTGACGCAAAAAC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 105 nt
>oriT_pKQPS2
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 856 | GenBank | WP_004206924 |
| Name | PutativerelaxaseofpKQPS2 |
UniProt ID | _ |
| Length | 659 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75087.84 Da Isoelectric Point: 10.0285
>WP_004206924.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 41603..60814
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AVR78_RS28260 (AVR78_27450) | 36687..36998 | + | 312 | WP_004187333 | hypothetical protein | - |
| AVR78_RS28265 (AVR78_27455) | 37131..37667 | + | 537 | WP_004206920 | hypothetical protein | - |
| AVR78_RS28270 (AVR78_27460) | 38168..38533 | - | 366 | WP_004206922 | hypothetical protein | - |
| AVR78_RS28275 (AVR78_27465) | 38809..39126 | + | 318 | WP_004206923 | plasmid mobilization protein MobA | - |
| AVR78_RS28280 (AVR78_27470) | 39113..41092 | + | 1980 | WP_004206924 | TraI/MobA(P) family conjugative relaxase | - |
| AVR78_RS28285 (AVR78_27475) | 41106..41606 | + | 501 | WP_004206925 | DotD/TraH family lipoprotein | - |
| AVR78_RS28290 (AVR78_27480) | 41603..42382 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| AVR78_RS28295 (AVR78_27485) | 42393..43556 | + | 1164 | WP_004206926 | plasmid transfer ATPase TraJ | virB11 |
| AVR78_RS28300 (AVR78_27490) | 43546..43806 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| AVR78_RS28305 (AVR78_27635) | 43831..44631 | + | 801 | WP_072500849 | DUF5710 domain-containing protein | - |
| AVR78_RS30465 | 44606..46039 | + | 1434 | WP_227520075 | LPD7 domain-containing protein | - |
| AVR78_RS30470 | 46236..46484 | + | 249 | WP_227520076 | hypothetical protein | - |
| AVR78_RS30475 | 46517..46726 | + | 210 | WP_227520077 | hypothetical protein | - |
| AVR78_RS30480 | 46776..47165 | + | 390 | WP_227520078 | hypothetical protein | - |
| AVR78_RS28315 | 47189..47623 | + | 435 | WP_159376490 | hypothetical protein | traL |
| AVR78_RS28320 | 47624..47836 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| AVR78_RS28325 (AVR78_27500) | 47808..48218 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| AVR78_RS30485 | 48649..48864 | + | 216 | WP_227520079 | hypothetical protein | traM |
| AVR78_RS30505 | 49013..49906 | + | 894 | WP_148663137 | DotH/IcmK family type IV secretion protein | traN |
| AVR78_RS30510 (AVR78_27510) | 49923..50165 | + | 243 | WP_158513517 | DotH/IcmK family type IV secretion protein | traN |
| AVR78_RS28345 (AVR78_27515) | 50177..51526 | + | 1350 | WP_004206932 | conjugal transfer protein TraO | traO |
| AVR78_RS28350 (AVR78_27520) | 51538..52242 | + | 705 | WP_004206933 | conjugal transfer protein TraP | traP |
| AVR78_RS28355 (AVR78_27525) | 52266..52796 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| AVR78_RS28360 (AVR78_27530) | 52813..53202 | + | 390 | WP_011154472 | DUF6750 family protein | traR |
| AVR78_RS28365 (AVR78_27535) | 53248..53742 | + | 495 | WP_004187480 | hypothetical protein | - |
| AVR78_RS28370 (AVR78_27540) | 53739..56789 | + | 3051 | WP_004206934 | hypothetical protein | traU |
| AVR78_RS28375 (AVR78_27545) | 56786..57994 | + | 1209 | WP_004187486 | conjugal transfer protein TraW | traW |
| AVR78_RS28380 (AVR78_27550) | 57991..58641 | + | 651 | WP_004187488 | hypothetical protein | - |
| AVR78_RS28385 (AVR78_27555) | 58634..60814 | + | 2181 | WP_004187492 | DotA/TraY family protein | traY |
| AVR78_RS28390 (AVR78_27560) | 60817..61470 | + | 654 | WP_004206935 | hypothetical protein | - |
| AVR78_RS28395 (AVR78_27565) | 61544..61774 | + | 231 | WP_004187496 | IncL/M type plasmid replication protein RepC | - |
| AVR78_RS28400 (AVR78_27570) | 62071..63126 | + | 1056 | WP_015062846 | plasmid replication initiator RepA | - |
| AVR78_RS28405 (AVR78_27575) | 64298..64831 | - | 534 | Protein_85 | IS110-like element IS4321 family transposase | - |
Host bacterium
| ID | 1244 | GenBank | NZ_CP014698 |
| Plasmid name | pKQPS2 | Incompatibility group | IncL/M |
| Plasmid size | 77127 bp | Coordinate of oriT [Strand] | 38701..38805 [+] |
| Host baterium | Klebsiella quasipneumoniae strain ATCC 700603 isolate K6 |
Cargo genes
| Drug resistance gene | blaSHV-18, aadA6, blaOXA-2, qacE, sul1 |
| Virulence gene | - |
| Metal resistance gene | merR, merE, merD, merA, merC, merP, merT |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |