Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100783 |
| Name | oriT_pSAN1-2010K-2577  |
| Organism | Salmonella enterica subsp. enterica serovar Anatum str. USDA-ARS-USMARC-1783 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP014662 (50510..50612 [-], 103 nt) |
| oriT length | 103 nt |
| IRs (inverted repeats) | IR1: 19..26, 30..37 (GTCGGGGC..GCCCTGAC) IR2: 44..58, 67..81 (GCAATTGTATAATCG..CGGTTATACAATTGC) |
| Location of nic site | 89..90 |
| Conserved sequence flanking the nic site |
CATCCTG|T |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 103 nt
>oriT_pSAN1-2010K-2577
TCAGCTCCTTACTGGGGTGTCGGGGCGAAGCCCTGACCAGGAGGCAATTGTATAATCGCGCGTGCGCGGTTATACAATTGCACATCCTGTCCCGTTTTTCAGG
TCAGCTCCTTACTGGGGTGTCGGGGCGAAGCCCTGACCAGGAGGCAATTGTATAATCGCGCGTGCGCGGTTATACAATTGCACATCCTGTCCCGTTTTTCAGG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 855 | GenBank | WP_001354015 |
| Name | NikB_pSAN1-2010K-2577 |
UniProt ID | _ |
| Length | 899 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 899 a.a. Molecular weight: 104018.47 Da Isoelectric Point: 7.3526
>WP_001354015.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCLAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPENRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCLAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPENRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 55978..93744
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AW46_RS22930 (AW46_22930) | 53694..55985 | - | 2292 | WP_001289276 | F-type conjugative transfer protein TrbC | - |
| AW46_RS22935 (AW46_22935) | 55978..57048 | - | 1071 | WP_000151583 | IncI1-type conjugal transfer protein TrbB | trbB |
| AW46_RS22940 (AW46_22940) | 57067..58275 | - | 1209 | WP_000121274 | IncI1-type conjugal transfer protein TrbA | trbA |
| AW46_RS22945 (AW46_22945) | 58567..58719 | + | 153 | WP_001331364 | Hok/Gef family protein | - |
| AW46_RS26025 | 58791..58982 | - | 192 | WP_174715448 | hypothetical protein | - |
| AW46_RS25985 | 59543..59638 | + | 96 | WP_000609148 | DinQ-like type I toxin DqlB | - |
| AW46_RS25610 | 59703..59879 | - | 177 | WP_001054904 | hypothetical protein | - |
| AW46_RS22955 (AW46_22955) | 60271..60480 | + | 210 | WP_000062603 | HEAT repeat domain-containing protein | - |
| AW46_RS22960 (AW46_22960) | 60552..61214 | - | 663 | WP_000653334 | plasmid IncI1-type surface exclusion protein ExcA | - |
| AW46_RS22965 (AW46_22965) | 61279..63441 | - | 2163 | WP_000698351 | DotA/TraY family protein | traY |
| AW46_RS22970 (AW46_22970) | 63538..64122 | - | 585 | WP_001037987 | IncI1-type conjugal transfer protein TraX | - |
| AW46_RS22975 (AW46_22975) | 64151..65353 | - | 1203 | WP_024261752 | IncI1-type conjugal transfer protein TraW | traW |
| AW46_RS24605 | 65320..65934 | - | 615 | WP_000337398 | IncI1-type conjugal transfer protein TraV | traV |
| AW46_RS22980 (AW46_22980) | 65934..68978 | - | 3045 | WP_001024780 | IncI1-type conjugal transfer protein TraU | traU |
| AW46_RS22985 (AW46_22985) | 69068..69868 | - | 801 | WP_001164788 | IncI1-type conjugal transfer protein TraT | traT |
| AW46_RS22990 (AW46_22990) | 69852..70040 | - | 189 | WP_001277255 | putative conjugal transfer protein TraS | - |
| AW46_RS22995 (AW46_22995) | 70104..70508 | - | 405 | WP_000086957 | IncI1-type conjugal transfer protein TraR | traR |
| AW46_RS23000 (AW46_23000) | 70559..71086 | - | 528 | WP_001055569 | conjugal transfer protein TraQ | traQ |
| AW46_RS23005 (AW46_23005) | 71086..71790 | - | 705 | WP_000801920 | IncI1-type conjugal transfer protein TraP | traP |
| AW46_RS23010 (AW46_23010) | 71790..73079 | - | 1290 | WP_001272005 | conjugal transfer protein TraO | traO |
| AW46_RS23015 (AW46_23015) | 73082..74065 | - | 984 | WP_001191877 | IncI1-type conjugal transfer protein TraN | traN |
| AW46_RS23020 (AW46_23020) | 74076..74768 | - | 693 | WP_000138548 | DotI/IcmL family type IV secretion protein | traM |
| AW46_RS23025 (AW46_23025) | 74765..75112 | - | 348 | WP_001055900 | conjugal transfer protein | traL |
| AW46_RS23030 (AW46_23030) | 75130..78897 | - | 3768 | WP_001141529 | LPD7 domain-containing protein | - |
| AW46_RS23035 (AW46_23035) | 78987..79538 | - | 552 | WP_000014583 | phospholipase D family protein | - |
| AW46_RS24610 | 79553..79843 | - | 291 | WP_001299214 | hypothetical protein | traK |
| AW46_RS23040 (AW46_23040) | 79840..80988 | - | 1149 | WP_001024972 | plasmid transfer ATPase TraJ | virB11 |
| AW46_RS23045 (AW46_23045) | 80985..81803 | - | 819 | WP_000646098 | IncI1-type conjugal transfer lipoprotein TraI | traI |
| AW46_RS23050 (AW46_23050) | 81800..82258 | - | 459 | WP_001079808 | IncI1-type conjugal transfer lipoprotein TraH | - |
| AW46_RS23055 (AW46_23055) | 82653..83237 | - | 585 | WP_000977522 | histidine phosphatase family protein | - |
| AW46_RS23060 (AW46_23060) | 83297..84499 | - | 1203 | WP_000976351 | conjugal transfer protein TraF | - |
| AW46_RS23065 (AW46_23065) | 84584..85408 | - | 825 | WP_001238939 | conjugal transfer protein TraE | traE |
| AW46_RS23070 (AW46_23070) | 85559..86713 | - | 1155 | WP_001139957 | site-specific integrase | - |
| AW46_RS25940 | 86712..87065 | + | 354 | WP_001393368 | hypothetical protein | - |
| AW46_RS25425 | 87701..87949 | + | 249 | WP_001349157 | hypothetical protein | - |
| AW46_RS23075 (AW46_23075) | 87946..89238 | - | 1293 | WP_001417545 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| AW46_RS24645 | 89238..89894 | - | 657 | WP_001193553 | prepilin peptidase | - |
| AW46_RS23080 (AW46_23080) | 89879..90439 | - | 561 | WP_000014116 | lytic transglycosylase domain-containing protein | virB1 |
| AW46_RS23085 (AW46_23085) | 90449..91063 | - | 615 | WP_000959785 | type 4 pilus major pilin | - |
| AW46_RS23090 (AW46_23090) | 91081..92178 | - | 1098 | WP_001208805 | type II secretion system F family protein | - |
| AW46_RS23095 (AW46_23095) | 92191..93744 | - | 1554 | WP_000362202 | ATPase, T2SS/T4P/T4SS family | virB11 |
| AW46_RS23100 (AW46_23100) | 93755..94207 | - | 453 | WP_001247336 | type IV pilus biogenesis protein PilP | - |
| AW46_RS23105 (AW46_23105) | 94194..95489 | - | 1296 | WP_000752774 | type 4b pilus protein PilO2 | - |
| AW46_RS23110 (AW46_23110) | 95482..97164 | - | 1683 | WP_000748143 | PilN family type IVB pilus formation outer membrane protein | - |
| AW46_RS23115 (AW46_23115) | 97178..97615 | - | 438 | WP_000539807 | type IV pilus biogenesis protein PilM | - |
| AW46_RS24650 | 97615..98682 | - | 1068 | WP_000742600 | type IV pilus biogenesis lipoprotein PilL | - |
Host bacterium
| ID | 1243 | GenBank | NZ_CP014662 |
| Plasmid name | pSAN1-2010K-2577 | Incompatibility group | IncI1 |
| Plasmid size | 103851 bp | Coordinate of oriT [Strand] | 50510..50612 [-] |
| Host baterium | Salmonella enterica subsp. enterica serovar Anatum str. USDA-ARS-USMARC-1783 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |