Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100779 |
| Name | oriT_pZH063_2  |
| Organism | Escherichia coli strain ZH063 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP014524 (36021..36647 [+], 627 nt) |
| oriT length | 627 nt |
| IRs (inverted repeats) | IR1: 198..207, 211..220 (ATTGGGTGTT..AATACCTAAT) IR2:319..324, 330..335 (AAAAAA..TTTTTT) IR3: 393..402, 405..414 (GTATTCATGC..GCATGAATAC) |
| Location of nic site | 301..302 |
| Conserved sequence flanking the nic site |
GGTGT|ATAGC |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 627 nt
>oriT_pZH063_2
AGCGCCGCAGATAATCTGACCGATTACCTCCTGAAACCAGGTCTATATAGGCCAAAAGTTCATCTGATACTTTTGCGGTTATTATTGGCATTCAGTCCTCACATTGTGCATTTTTTAAACAAAAAATTGGGATCTAACAAGCTGAAATCTTAGTATTACCAAAGTAATAAAGCAAACTCATTATAAAACAATGAGTTATTGGGTGTTTTTAATACCTAATTATTACCGAATATTGTTGCTATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAGGGGCGATCTACGTAGGTTAAGGACTAACTGGCTAAAAAGCGTTCAATATTCCGTATTCATGCTTGCATGAATACCAGTACAACAAAAGTACATCAAAATTACATCAAAATTACATCACTTGAAGGTTGACAGTACAACAAAATTACATCATTCTTTGGTCATGAGGTAGCCAGTACAACAAAAGTACATCAAAAGTACATCAAAATTACATCAAAATTACATCATTCTAAATGAGGGTACTATGAAGCCCAAAAGTATCAGGGCGGCACTTCAGTTGATGTTGCCGG
AGCGCCGCAGATAATCTGACCGATTACCTCCTGAAACCAGGTCTATATAGGCCAAAAGTTCATCTGATACTTTTGCGGTTATTATTGGCATTCAGTCCTCACATTGTGCATTTTTTAAACAAAAAATTGGGATCTAACAAGCTGAAATCTTAGTATTACCAAAGTAATAAAGCAAACTCATTATAAAACAATGAGTTATTGGGTGTTTTTAATACCTAATTATTACCGAATATTGTTGCTATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAGGGGCGATCTACGTAGGTTAAGGACTAACTGGCTAAAAAGCGTTCAATATTCCGTATTCATGCTTGCATGAATACCAGTACAACAAAAGTACATCAAAATTACATCAAAATTACATCACTTGAAGGTTGACAGTACAACAAAATTACATCATTCTTTGGTCATGAGGTAGCCAGTACAACAAAAGTACATCAAAAGTACATCAAAATTACATCAAAATTACATCATTCTAAATGAGGGTACTATGAAGCCCAAAAGTATCAGGGCGGCACTTCAGTTGATGTTGCCGG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 851 | GenBank | WP_001144737 |
| Name | MobC_pZH063_2 |
UniProt ID | _ |
| Length | 88 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 88 a.a. Molecular weight: 10315.57 Da Isoelectric Point: 5.5791
>WP_001144737.1 MULTISPECIES: plasmid mobilization relaxosome protein MobC [Gammaproteobacteria]
MPVLTDEEVNTVRESCRQLGAIGRNLNQVARALNIEFRESDKLKQEAIEKLAERIDQHLDHVSELFDKTW
SRWHDCHFQKTTAPVDWA
MPVLTDEEVNTVRESCRQLGAIGRNLNQVARALNIEFRESDKLKQEAIEKLAERIDQHLDHVSELFDKTW
SRWHDCHFQKTTAPVDWA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 12226..22550
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AVR76_RS25825 (AVR76_25840) | 8071..9504 | - | 1434 | WP_001288432 | DNA cytosine methyltransferase | - |
| AVR76_RS25830 (AVR76_25845) | 9603..9875 | + | 273 | Protein_10 | IS1 family transposase | - |
| AVR76_RS25835 (AVR76_25850) | 9886..10092 | - | 207 | WP_001749967 | hypothetical protein | - |
| AVR76_RS25840 (AVR76_25855) | 10097..10744 | - | 648 | WP_015344958 | restriction endonuclease | - |
| AVR76_RS25845 (AVR76_25860) | 10794..11105 | - | 312 | WP_001452736 | hypothetical protein | - |
| AVR76_RS25850 (AVR76_25865) | 11141..11455 | - | 315 | WP_001749965 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| AVR76_RS25855 (AVR76_25870) | 11452..11796 | - | 345 | WP_001749964 | hypothetical protein | - |
| AVR76_RS25860 (AVR76_25875) | 11812..12117 | - | 306 | WP_000960954 | H-NS family nucleoid-associated regulatory protein | - |
| AVR76_RS25865 (AVR76_25880) | 12226..12960 | + | 735 | WP_001749963 | lytic transglycosylase domain-containing protein | virB1 |
| AVR76_RS25870 (AVR76_25885) | 12969..13250 | + | 282 | WP_000440698 | transcriptional repressor KorA | - |
| AVR76_RS25875 (AVR76_25890) | 13260..13553 | + | 294 | WP_001749962 | hypothetical protein | virB2 |
| AVR76_RS25880 (AVR76_25895) | 13603..13920 | + | 318 | WP_000496058 | VirB3 family type IV secretion system protein | virB3 |
| AVR76_RS25885 (AVR76_25900) | 13920..16520 | + | 2601 | WP_001749961 | VirB4 family type IV secretion/conjugal transfer ATPase | virb4 |
| AVR76_RS25890 (AVR76_25905) | 16538..17251 | + | 714 | WP_001749960 | type IV secretion system protein | virB5 |
| AVR76_RS25895 (AVR76_25910) | 17259..17486 | + | 228 | WP_001749959 | IncN-type entry exclusion lipoprotein EexN | - |
| AVR76_RS25900 (AVR76_25915) | 17502..18542 | + | 1041 | WP_001749958 | type IV secretion system protein | virB6 |
| AVR76_RS25905 (AVR76_25920) | 18625..18771 | + | 147 | WP_001257173 | conjugal transfer protein TraN | - |
| AVR76_RS25910 (AVR76_25925) | 18761..19459 | + | 699 | WP_000646594 | type IV secretion system protein | virB8 |
| AVR76_RS25915 (AVR76_25930) | 19470..20354 | + | 885 | WP_000735066 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| AVR76_RS25920 (AVR76_25935) | 20354..21513 | + | 1160 | Protein_28 | type IV secretion system protein VirB10 | - |
| AVR76_RS25925 (AVR76_25940) | 21555..22550 | + | 996 | WP_000128596 | ATPase, T2SS/T4P/T4SS family | virB11 |
| AVR76_RS25930 (AVR76_25945) | 22550..23083 | + | 534 | WP_000792636 | phospholipase D family protein | - |
| AVR76_RS30075 (AVR76_25950) | 23257..23544 | - | 288 | Protein_31 | DUF6710 family protein | - |
| AVR76_RS25940 (AVR76_25955) | 23811..23990 | + | 180 | Protein_32 | helix-turn-helix domain-containing protein | - |
| AVR76_RS25945 (AVR76_25960) | 24055..24759 | + | 705 | WP_001067855 | IS6-like element IS26 family transposase | - |
| AVR76_RS28015 | 24812..25435 | - | 624 | Protein_34 | DUF4158 domain-containing protein | - |
| AVR76_RS25950 (AVR76_25965) | 25530..26135 | + | 606 | WP_000509966 | recombinase family protein | - |
| AVR76_RS25955 (AVR76_25970) | 26168..26416 | - | 249 | Protein_36 | IS6 family transposase | - |
| AVR76_RS25960 (AVR76_25975) | 26428..27084 | - | 657 | WP_000113282 | tetracycline resistance transcriptional repressor TetR(D) | - |
Host bacterium
| ID | 1239 | GenBank | NZ_CP014524 |
| Plasmid name | pZH063_2 | Incompatibility group | IncN |
| Plasmid size | 49467 bp | Coordinate of oriT [Strand] | 36021..36647 [+] |
| Host baterium | Escherichia coli strain ZH063 |
Cargo genes
| Drug resistance gene | dfrA14, tet(D) |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |