Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100777
• Name: oriT_pUSA06-1-SUR10
• Organism: Staphylococcus aureus strain USA300-SUR10
• Sequence Completeness: core
• NCBI accession of oriT (coordinates [strand]): NZ_CP014401 (4022..4074 [-], 53 nt)
• oriT length: 53 nt
• IRs (inverted repeats): 12..19, 35..42 (GTATATTC..GAATATAC)
• Location of nic site: 50..51
• Conserved sequence flanking the nic site: CGCTTG|CCA
• Note: predicted by the oriTfinder

oriT sequence

• Length: 53 nt

>oriT_pUSA06-1-SUR10
CCAGCAAGCCGGTATATTCAGAACGAAGTGGCTAGAATATACGACGCTTGCCA

oriT secondary structure

Predicted by RNAfold.

Relaxase

Rlx_pUSA06-1-SUR10

• ID: 849
• GenBank: WP_000246202
• Name: Rlx_pUSA06-1-SUR10
• UniProt ID: A0A0U2YPT6
• Length: 320 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 320 a.a.
• Molecular weight: 36992.94 Da
• Isoelectric Point: 5.4336

>WP_000246202.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Staphylococcaceae]
MATTKLGNTKSASRAINYAEERAEEKSGLNCDVDYAKSYFKQTRALYGKENGVQAHTVIQSFKPGEVTAK
ECNEIGLELAKKIAPDYQVAVYTHTDKDHYHNHIIINSVNLETGNKYQSNKEQRDFIKKANDQLCEERGL
SVPEKSSEIRYTLAEQNMIDKDKRSWKNDIRMAVEETKDNAVDFEEFNTLLKEKGVEITRVTKNNVTYRH
IEEDKKVRGNKLGDSYDKGVIENGFAIEKFRREREEEREYDEYADTFEVDWDAVAENSEDLRKRRIARTE
ETKQASNKIYIRDERTTGLERKGIAGNQVEFEKDDGGLSR

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (8-235)

Protein structure

• AlphaFold DB: A0A0U2YPT6

Host bacterium

• ID: 1237
• GenBank: NZ_CP014401
• Plasmid name: pUSA06-1-SUR10
• Incompatibility group: _
• Plasmid size: 4397 bp
• Coordinate of oriT [Strand]: 4022..4074 [-]
• Host baterium: Staphylococcus aureus strain USA300-SUR10

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -