Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100774
• Name: oriT_pFOL01
• Organism: Weissella jogaejeotgali strain FOL01
• Sequence Completeness: incomplete
• NCBI accession of oriT (coordinates [strand]): NZ_CP014333 (24918..24975 [+], 58 nt)
• oriT length: 58 nt
• IRs (inverted repeats): 1..27, 32..58 (AATTGATTTATTATGTGAGTAGAAACC.. GGGTTTCACGAGCTTAATCAATCAATT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: predicted by the oriTfinder

oriT sequence

• Length: 58 nt

>oriT_pFOL01
AATTGATTTATTATGTGAGTAGAAACCCTTAGGGTTTCACGAGCTTAATCAATCAATT

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_pFOL01

• ID: 846
• GenBank: WP_075270664
• Name: Relaxase_pFOL01
• UniProt ID: A0A1L6REE8
• Length: 390 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 390 a.a.
• Molecular weight: 45395.01 Da
• Isoelectric Point: 9.9021

>WP_075270664.1 relaxase/mobilization nuclease domain-containing protein [Weissella jogaejeotgali]
MATTHIKRSASASRLVNYAEKRAVLKDGLNLDVDYAKSQFKQVREVYGNQGKTQAYASRISFSPKEFDPT
NEDDQQKALDIAKEVYQKTYPNQQVALYEHADTGALHIHAVVGAIDLETGKKMHGNWQEYREKLVHNTDE
IVQEHGLAVTQVNPERYEKRSMAEIKMQDRGQPTWKDQIRQAVDSTMSNQLIHDFQTFRDDLKQKAIDVW
ERGKDLTYQLTGTNYKARGKKLGTAYEKGAIYHELERRTRDPRDTQSETRKPDTTRIDSSQSDFERNQEL
SGYTNSTPEQTANRVDRTAPTNRTNQPTRQSSLSDDLEQFRKQQQDQQRTISRDAKRRAKTVQRTDNDHQ
SKDNRRTQTDKPRTKEQQRYPEDLKRQPKPNRSKDYGPSR

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (9-248)

Protein structure

• AlphaFold DB: A0A1L6REE8

Host bacterium

• ID: 1234
• GenBank: NZ_CP014333
• Plasmid name: pFOL01
• Incompatibility group: _
• Plasmid size: 35382 bp
• Coordinate of oriT [Strand]: 24918..24975 [+]
• Host baterium: Weissella jogaejeotgali strain FOL01

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -