Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100768 |
| Name | oriT_pKP38731-2  |
| Organism | Klebsiella pneumoniae strain KP38731 |
| Sequence Completeness | core |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP014298 (31515..31620 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 18..35, 41..58 (GTTTTTGGAGTACCGCCG..CGGCGGCCGTCCAAAAAC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 106 nt
>oriT_pKP38731-2
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 840 | GenBank | WP_050868819 |
| Name | MobA_pKP38731-2 |
UniProt ID | A0A142BMF1 |
| Length | 659 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75234.98 Da Isoelectric Point: 10.0285
>WP_050868819.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPVYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSERDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPVYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSERDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A142BMF1 |
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 34416..50695
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AXK16_RS29370 | 29501..29811 | + | 311 | Protein_48 | hypothetical protein | - |
| AXK16_RS29375 (AXK16_29455) | 29944..30480 | + | 537 | WP_011091066 | hypothetical protein | - |
| AXK16_RS29380 (AXK16_29460) | 30981..31346 | - | 366 | WP_011091067 | hypothetical protein | - |
| AXK16_RS29385 (AXK16_29465) | 31622..31939 | + | 318 | WP_011091068 | plasmid mobilization protein MobA | - |
| AXK16_RS29390 (AXK16_29470) | 31926..33905 | + | 1980 | WP_050868819 | TraI/MobA(P) family conjugative relaxase | - |
| AXK16_RS29395 (AXK16_29475) | 33919..34419 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| AXK16_RS29400 (AXK16_29480) | 34416..35195 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| AXK16_RS29405 (AXK16_29485) | 35206..36369 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| AXK16_RS29410 (AXK16_29490) | 36359..36619 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| AXK16_RS29415 (AXK16_29495) | 38180..39855 | + | 1676 | Protein_57 | hypothetical protein | - |
| AXK16_RS29420 | 39821..40333 | + | 513 | WP_011091071 | hypothetical protein | traL |
| AXK16_RS29425 | 40334..40531 | - | 198 | WP_004187464 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| AXK16_RS29430 (AXK16_29500) | 40518..40928 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| AXK16_RS29435 (AXK16_29505) | 40927..41709 | + | 783 | WP_011091073 | DotI/IcmL family type IV secretion protein | traM |
| AXK16_RS29440 | 41718..42665 | + | 948 | WP_148663877 | DotH/IcmK family type IV secretion protein | traN |
| AXK16_RS30685 (AXK16_29515) | 42596..42868 | + | 273 | WP_148663878 | DotH/IcmK family type IV secretion protein | traN |
| AXK16_RS29445 (AXK16_29520) | 42880..44229 | + | 1350 | WP_011091075 | conjugal transfer protein TraO | traO |
| AXK16_RS29450 (AXK16_29525) | 44241..44944 | + | 704 | Protein_65 | conjugal transfer protein TraP | - |
| AXK16_RS29455 (AXK16_29530) | 44968..45498 | + | 531 | WP_011091077 | conjugal transfer protein TraQ | traQ |
| AXK16_RS29460 (AXK16_29535) | 45515..45904 | + | 390 | WP_011091078 | DUF6750 family protein | traR |
| AXK16_RS29465 (AXK16_29540) | 45950..46444 | + | 495 | WP_011091080 | hypothetical protein | - |
| AXK16_RS29470 (AXK16_29545) | 46441..49490 | + | 3050 | Protein_69 | conjugal transfer protein | - |
| AXK16_RS29475 (AXK16_29550) | 49487..50695 | + | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| AXK16_RS29480 (AXK16_29555) | 50692..51342 | + | 651 | WP_011091083 | hypothetical protein | - |
| AXK16_RS29485 (AXK16_29560) | 51335..53514 | + | 2180 | Protein_72 | DotA/TraY family protein | - |
| AXK16_RS29490 (AXK16_29565) | 53517..54169 | + | 653 | Protein_73 | ExcA | - |
Host bacterium
| ID | 1228 | GenBank | NZ_CP014298 |
| Plasmid name | pKP38731-2 | Incompatibility group | IncL/M |
| Plasmid size | 70438 bp | Coordinate of oriT [Strand] | 31515..31620 [+] |
| Host baterium | Klebsiella pneumoniae strain KP38731 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |