Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100767 |
| Name | oriT_pKP38731-1  |
| Organism | Klebsiella pneumoniae strain KP38731 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP014295 (128671..129221 [-], 551 nt) |
| oriT length | 551 nt |
| IRs (inverted repeats) | IR1: 62..70, 71..79 (AACCCTTTC..GACAGGGTT) IR2: 143..150, 154..161 (TGGCCTGC..GGAGGCCA) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 551 nt
>oriT_pKP38731-1
TGTAAAGAGGCTGTGAAAGAATAAGAGCATCAAGATTCCAGATAGATAGAGGGAAATTTGACAAATTCCAAAGATGGGTTAGCCTAGTGACAGAACTAGATTCCAGTATTGGAATAATCAGCTTTAAATTCCAGATAGATAGTTATGTGGATAGGAATTGGATAGGAATTGGGAGGGTATTGAGG
TGTAAAGAGGCTGTGAAAGAATAAGAGCATCAAGATTCCAGATAGATAGAGGGAAATTTGACAAATTCCAAAGATGGGTTAGCCTAGTGACAGAACTAGATTCCAGTATTGGAATAATCAGCTTTAAATTCCAGATAGATAGTTATGTGGATAGGAATTGGATAGGAATTGGGAGGGTATTGAGG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 839 | GenBank | WP_001337757 |
| Name | TraI_pKP38731-1 |
UniProt ID | _ |
| Length | 992 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 992 a.a. Molecular weight: 109581.85 Da Isoelectric Point: 4.8956
>WP_001337757.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGPTESSK
PDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPF
DAFNASAETTSTDATNSEIPDVAMPGKQEEQPKQDFVPQEQNSLQGDDFPMFGGSDEPPSWAIEPLPMLT
DAPEQPTHTPEMPHTDNVNQHEKDAKTLLVEMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVI
LYPDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQ
DAFELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQ
RKPVQEKQNVARLPKREAQPVAPEPKVEREKELGHVEVREREDPEVREFEPPKAKTNPKDINAEDFLPSG
VTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKK
RQGKLYLEVNET
MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGPTESSK
PDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPF
DAFNASAETTSTDATNSEIPDVAMPGKQEEQPKQDFVPQEQNSLQGDDFPMFGGSDEPPSWAIEPLPMLT
DAPEQPTHTPEMPHTDNVNQHEKDAKTLLVEMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVI
LYPDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQ
DAFELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQ
RKPVQEKQNVARLPKREAQPVAPEPKVEREKELGHVEVREREDPEVREFEPPKAKTNPKDINAEDFLPSG
VTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKK
RQGKLYLEVNET
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 103969..131087
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AXK16_RS27965 (AXK16_29055) | 99096..100004 | - | 909 | WP_000739139 | hypothetical protein | - |
| AXK16_RS27970 (AXK16_29060) | 100015..100983 | - | 969 | WP_000085162 | AAA family ATPase | - |
| AXK16_RS27975 (AXK16_29065) | 101203..101388 | - | 186 | WP_001186917 | hypothetical protein | - |
| AXK16_RS27980 (AXK16_29070) | 101692..102012 | - | 321 | WP_000547566 | hypothetical protein | - |
| AXK16_RS27985 (AXK16_29075) | 102306..102947 | + | 642 | WP_000796664 | hypothetical protein | - |
| AXK16_RS27990 (AXK16_29080) | 103070..103930 | + | 861 | WP_000709517 | hypothetical protein | - |
| AXK16_RS27995 (AXK16_29085) | 103969..106779 | - | 2811 | WP_001909286 | conjugal transfer mating pair stabilization protein TraN | traN |
| AXK16_RS28000 (AXK16_29090) | 106883..107890 | - | 1008 | WP_000983283 | TraU family protein | traU |
| AXK16_RS28005 (AXK16_29095) | 107887..108558 | - | 672 | WP_001337754 | EAL domain-containing protein | - |
| AXK16_RS28010 (AXK16_29100) | 108555..109820 | - | 1266 | WP_000621288 | TrbC family F-type conjugative pilus assembly protein | traW |
| AXK16_RS28015 (AXK16_29105) | 109783..110313 | - | 531 | WP_001010738 | S26 family signal peptidase | - |
| AXK16_RS28020 (AXK16_29110) | 110310..110627 | - | 318 | WP_000351984 | hypothetical protein | - |
| AXK16_RS28025 (AXK16_29115) | 110642..113089 | - | 2448 | WP_000637386 | type IV secretion system protein TraC | virb4 |
| AXK16_RS28030 (AXK16_29120) | 113086..113793 | - | 708 | WP_001259347 | DsbC family protein | - |
| AXK16_RS28035 (AXK16_29125) | 113967..115373 | - | 1407 | WP_082261857 | group II intron reverse transcriptase/maturase | - |
| AXK16_RS28040 (AXK16_29130) | 115872..121403 | - | 5532 | WP_000606833 | Ig-like domain-containing protein | - |
| AXK16_RS28045 (AXK16_29135) | 121604..121996 | - | 393 | WP_000479535 | TraA family conjugative transfer protein | - |
| AXK16_RS28050 (AXK16_29140) | 122000..122578 | - | 579 | WP_000793435 | type IV conjugative transfer system lipoprotein TraV | traV |
| AXK16_RS28055 (AXK16_29145) | 122575..123890 | - | 1316 | Protein_154 | conjugal transfer protein TraB | - |
| AXK16_RS28060 (AXK16_29150) | 123887..124804 | - | 918 | WP_000794249 | type-F conjugative transfer system secretin TraK | traK |
| AXK16_RS28065 (AXK16_29155) | 124788..125414 | - | 627 | WP_001049716 | TraE/TraK family type IV conjugative transfer system protein | traE |
| AXK16_RS28070 (AXK16_29160) | 125411..125691 | - | 281 | Protein_157 | type IV conjugative transfer system protein TraL | - |
| AXK16_RS28075 (AXK16_29165) | 125836..126213 | - | 378 | WP_000509509 | hypothetical protein | - |
| AXK16_RS28080 (AXK16_29170) | 126537..127127 | - | 591 | WP_002210536 | hypothetical protein | - |
| AXK16_RS28085 (AXK16_29175) | 127198..127575 | - | 378 | WP_000869261 | hypothetical protein | - |
| AXK16_RS28090 (AXK16_29180) | 127585..128031 | - | 447 | WP_000122506 | hypothetical protein | - |
| AXK16_RS28095 (AXK16_29185) | 128041..128670 | - | 630 | WP_000743450 | DUF4400 domain-containing protein | tfc7 |
| AXK16_RS28100 (AXK16_29190) | 128627..129211 | - | 585 | WP_000332866 | hypothetical protein | - |
| AXK16_RS28105 (AXK16_29195) | 129222..131087 | - | 1866 | WP_000178856 | conjugative transfer system coupling protein TraD | virb4 |
| AXK16_RS28110 (AXK16_29200) | 131084..134062 | - | 2979 | WP_001337757 | MobH family relaxase | - |
| AXK16_RS28115 (AXK16_29205) | 134230..134847 | + | 618 | WP_001249396 | hypothetical protein | - |
| AXK16_RS28120 (AXK16_29210) | 134829..135062 | + | 234 | WP_001191892 | hypothetical protein | - |
Host bacterium
| ID | 1227 | GenBank | NZ_CP014295 |
| Plasmid name | pKP38731-1 | Incompatibility group | IncA/C2 |
| Plasmid size | 137267 bp | Coordinate of oriT [Strand] | 128671..129221 [-] |
| Host baterium | Klebsiella pneumoniae strain KP38731 |
Cargo genes
| Drug resistance gene | tetracycline resistance |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |