Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100759
Name   oriT_pCAV1193-166 in_silico
Organism   Klebsiella pneumoniae strain CAV1193
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP013324 (18319..18869 [-], 551 nt)
oriT length   551 nt
IRs (inverted repeats)      IR1: 62..70, 71..79  (AACCCTTTC..GACAGGGTT)
  IR2: 143..150, 154..161  (TGGCCTGC..GGAGGCCA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 551 nt

>oriT_pCAV1193-166
GAGCAGAGCTATGTGTGACAAGAAGTATAGAAATTACGAGGTAGCCATCATGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAAGGCTAATCAGGAGCCTGTGATTTACCCGATCATTGAAGAAGCTCTGCATCGCTACAGCCAGCTCGTGTTTCATGAGCAGAGAGAGAAATATGAAGACCCGGCCAGAATTGGGGCATTTCTGGAAACCCTGATCACCGAAACCTGCCGGGCGTTGGAAGTGCAAATTGTCGATAGTGGCGGTGATTCATGGTCTGTCGATTCAGGAGAGTCGTTCTCACTGTGGCTTTCTTCCCATCCAGGAGAACTATCCATTAACCCGCAGCCCCATGAGGATGAGACCTCTTTGCGTGGCTTGCTGTATGAGCTCATCACCTGTGAGAGCGTGAAAACTGTTTTAAGGAGAACCGACT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   831 GenBank   WP_001326173
Name   TraI_pCAV1193-166 insolico UniProt ID   G9FJJ6
Length   990 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 990 a.a.        Molecular weight: 109356.90 Da        Isoelectric Point: 4.9395

>WP_001326173.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure


Source ID Structure
AlphaFold DB G9FJJ6


T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 175..20735

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AGG09_RS28015 (AGG09_00735) 175..1440 - 1266 WP_001447719 TrbC family F-type conjugative pilus assembly protein traW
AGG09_RS28020 (AGG09_00740) 1403..1933 - 531 WP_001010740 S26 family signal peptidase -
AGG09_RS28025 (AGG09_00745) 1930..2247 - 318 WP_000351984 hypothetical protein -
AGG09_RS28030 (AGG09_00750) 2262..4709 - 2448 WP_000637384 type IV secretion system protein TraC virb4
AGG09_RS28035 (AGG09_00755) 4706..5413 - 708 WP_001259346 DsbC family protein -
AGG09_RS28040 (AGG09_00760) 5562..11048 - 5487 WP_047718077 Ig-like domain-containing protein -
AGG09_RS28045 (AGG09_00765) 11249..11641 - 393 WP_000479535 TraA family conjugative transfer protein -
AGG09_RS28050 (AGG09_00770) 11645..12223 - 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
AGG09_RS28055 (AGG09_00775) 12220..13533 - 1314 WP_024131605 TraB/VirB10 family protein traB
AGG09_RS28060 (AGG09_00780) 13533..14450 - 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
AGG09_RS28065 (AGG09_00785) 14434..15060 - 627 WP_001049717 TraE/TraK family type IV conjugative transfer system protein traE
AGG09_RS28070 (AGG09_00790) 15057..15338 - 282 WP_000805625 type IV conjugative transfer system protein TraL traL
AGG09_RS28075 (AGG09_00795) 15483..15848 - 366 WP_001052530 hypothetical protein -
AGG09_RS28080 (AGG09_00800) 16184..16846 - 663 WP_001231464 hypothetical protein -
AGG09_RS28085 (AGG09_00805) 16846..17223 - 378 WP_000869297 hypothetical protein -
AGG09_RS28090 (AGG09_00810) 17233..17679 - 447 WP_000122507 hypothetical protein -
AGG09_RS28095 (AGG09_00815) 17689..18318 - 630 WP_000743449 DUF4400 domain-containing protein tfc7
AGG09_RS28100 (AGG09_00820) 18275..18820 - 546 WP_000228720 hypothetical protein -
AGG09_RS28105 (AGG09_00825) 18870..20735 - 1866 WP_000178857 conjugative transfer system coupling protein TraD virb4
AGG09_RS28110 (AGG09_00830) 20732..23704 - 2973 WP_001326173 MobH family relaxase -
AGG09_RS28115 (AGG09_00835) 23872..24489 + 618 WP_001249395 hypothetical protein -
AGG09_RS28120 (AGG09_00840) 24471..24704 + 234 WP_001191890 hypothetical protein -

Host bacterium

ID   1219 GenBank   NZ_CP013324
Plasmid name   pCAV1193-166 Incompatibility group   IncA/C2
Plasmid size   166486 bp Coordinate of oriT [Strand]   18319..18869 [-]
Host baterium   Klebsiella pneumoniae strain CAV1193

Cargo genes

Drug resistance gene   blaSHV-30, blaTEM-1A, blaOXA-9, ant(3'')-Ia, aac(6')-Ib, sul1, qnrA1, qacE, ant(2'')-Ia
Virulence gene   -
Metal resistance gene   merE, merD, merA, merC, merP, merT, merR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -