Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100756
• Name: oriT_PDM03
• Organism: Salmonella enterica subsp. enterica serovar Anatum strain GT-01
• Sequence Completeness: core
• NCBI accession of oriT (coordinates [strand]): NZ_CP013223 (344..404 [+], 61 nt)
• oriT length: 61 nt
• IRs (inverted repeats): 3..12, 16..25 (GTGTCGGGGC..GCCCTGACCC)
• Location of nic site: 57..58
• Conserved sequence flanking the nic site: CTGG|CTTA
• Note: predicted by the oriTfinder

oriT sequence

• Length: 61 nt

>oriT_PDM03
GGGTGTCGGGGCGCAGCCCTGACCCAGTTCACAGAGCGATAGCGATGTGTATACTGGCTTA

oriT secondary structure

Predicted by RNAfold.

Relaxase

Nuclease_PDM03

• ID: 828
• GenBank: WP_000635821
• Name: Nuclease_PDM03
• UniProt ID: _
• Length: 524 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 524 a.a.
• Molecular weight: 59269.83 Da
• Isoelectric Point: 9.9740

>WP_000635821.1 MULTISPECIES: nuclease [Bacteria]
MIVKFHARGKGGGSGPVDYLLGRERNREGATVLRGNPEEVRELIDSTPFSKKYTSGVLSFAEKELPPGER
ERVMTSFERVLMPGLEKDQYSILWVEHQDKGRLELNFVIPNMELQSGKRLQPYYDRADRPRINAWQTLVN
HHYGLRDPNAPENRRTLVTPNNLPKAKQEAAEAITRGLEALYHAGAIKTRQDVTEALTAAGFEVVRTTKS
SISIADPEGGRNLRLKGALYEQSFTNGNRLREETERASRFYREHAEQRVQEARRVCRQGIELKQAENQRR
YQRTYNIREKATGYAEHHREHAVQHHRGRTVPAAEKTALAGDQRADTVSGADRAQRHGRHFPGVLGADGV
AGKQDCEKYPDYQRAGKHAGETRGKNLGGDVRQWREREISGVAGRGERGKHLDGGRQERREAGQGVNEYD
GTGRAFAERLRAFATGLYAAAERVGERLRGIAEDARGYATEQRDTERARRDIESAGAEISRASGSLESVI
YREQQLRDEQERARYRHEVQERAARVRSGPSMEM

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (57-240)

Protein structure

No available structure.

Host bacterium

• ID: 1216
• GenBank: NZ_CP013223
• Plasmid name: PDM03
• Incompatibility group: _
• Plasmid size: 3371 bp
• Coordinate of oriT [Strand]: 344..404 [+]
• Host baterium: Salmonella enterica subsp. enterica serovar Anatum strain GT-01

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -