Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100749
Name   oriT_pKp04a in_silico
Organism   Klebsiella pneumoniae strain KpN01
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP012991 (2485..2677 [-], 193 nt)
oriT length   193 nt
IRs (inverted repeats)      22..29, 33..40  (TTCGGGGC..GCCCTGAA)
Location of nic site      73..74
Conserved sequence flanking the
  nic site    		  CTGG|CTAA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 193 nt

>oriT_pKp04a
GCGGCAGTGGTTTTAGCGGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTAAACATGTTATGACGGAGTGGTAATTTATGAAAGTGCTTCATGTGACAGGGGAAAAATGCGGCTGCCGGGCGTAAGTGCAAAATGCAGGTGGTGATGAAATGACGCCTCCTCGCTCAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   821 GenBank   WP_045265544
Name   PutativerelaxaseofpKp04a insolico UniProt ID   _
Length   497 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 497 a.a.        Molecular weight: 54828.84 Da        Isoelectric Point: 6.7956

>WP_045265544.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacterales]
MIVKFHPRGRGGGGGPVDYLLGKDRQRDGASVLQGKPDEVRELIDASPYAKKYTSGVLSFAEQDLPPGQR
EKLMASFERVLMPGLDKDQYSVLWVEHRDKGRLELNFLIPNTELLTGKRLQPYYDRADRPRIDAWQTIVN
GRLGLHDPNAPENRRVLVSPSALPEAKQEAAQAITSGLLALASSGELKTRQDVTEALESAGFEVVRTTKS
SISIADPDGGRNIRLKGAIYEQSFNAGEGLRAEIESAAADYRRDAESRIQRAREVCQSGTERKREENQRR
HPRPRPEAVLSHEPAHERDAAHGQPDVADHRSGLRAADSVERGHSVVAGAADTRELYDHPGAEEHAGHAV
REEQRRTALDLRRAETPLREGEPGSGTVRRGLELDDTGGEIAHDGAGKTVAERIRAATAGLLEKAGRVGE
RLRGMADDVWSYSTGERGAERARHGLEQAGAEFERAAAPVVERLNAIETHRQQERAVQHQKALELERSQW
QHHGPSL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(57-238)


  Protein structure



No available structure.




Host bacterium

ID   1209 GenBank   NZ_CP012991
Plasmid name   pKp04a Incompatibility group   ColRNAI
Plasmid size   3223 bp Coordinate of oriT [Strand]   2485..2677 [-]
Host baterium   Klebsiella pneumoniae strain KpN01

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -