Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100733
• Name: oriT_pCFSAN000679_01
• Organism: Salmonella enterica subsp. enterica serovar Choleraesuis str. ATCC 10708
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NZ_CP012345 (102856..103114 [-], 259 nt)
• oriT length: 259 nt
• IRs (inverted repeats): 115..128, 134..147 (ATAGCGGTGCCGGC..GCCTGCACCGCTAT)
• Location of nic site: 241..242
• Conserved sequence flanking the nic site: GTGGGGTGT|GG
• Note: predicted by the oriTfinder

oriT sequence

• Length: 259 nt

>oriT_pCFSAN000679_01
GCAGCGCCCCCAGGGGTGTTACTTAAAAACAGGCACAGCGACGCTAGCAGCGCCCCTGATATAATGCAATGTTTTTTATAAAAATAGTCAGTACTACCCCTGTATAGCGTCGTCATAGCGGTGCCGGCGACGTGCCTGCACCGCTATTTAATACTTTAAGATAAATTAAATTACATTATTATGAACATAACTCACTGTTACTAAAAACAAATCAGCAAAATTTTAATTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAG

oriT secondary structure

Predicted by RNAfold.

Relaxase

TraI_pCFSAN000679_01

• ID: 805
• GenBank: WP_053253528
• Name: TraI_pCFSAN000679_01
• UniProt ID: _
• Length: 1747 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 1747 a.a.
• Molecular weight: 190612.51 Da
• Isoelectric Point: 5.9711

>WP_053253528.1 conjugative transfer relaxase/helicase TraI [Salmonella enterica]
MMSIAQVRSAGSAAGYYSDRDNYYVLGSLEERWAGKGAEQLGLQGAVDKEVFTRVLEGRLPDGADLSRQQ
DGSNKHRPGYDLTFSAPKSVSLMAMLAGDKRLTEAHNQAVDIAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVANVTLHDGEWKTLSSDKVGKTGFIENVYANQIAFGKIYRAVLKEKVEA
LGYETEVVGKHGMWEMPGVPVEAFSSRSQVIREAVGEDASLKSRDVAALDTRKSKQHVDPEVKMAEWMQT
LKDTGFDISAYREAADRRAEIQVAQPAPSQEQPDIQQAVTQAIAGLSDRKVQFTYTDVLARTVGMLSPEA
GVIEKARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSSDIMKQNRVTIHPEKSVPRTGSYSD
AVSVLAQDRPSLAIISGQGGAAGQRERVAELTMMAREQGREVQIIAADRRSQTNLKQDERLSGELITGRR
QLQEGMSFSPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKEAGVNSYRWQ
GGQQTPATVISEPDRNVRYARLAGDFVAAVKAGEESVAQVSGVREQAILAGMIRSELKTQGILGQQDTMM
TALSPVWLDSRNRYLRDMYREGMVMEQWNPEKRSHDRYVIDRVTAQSHSLTLRDAQGETQMVRISALDSS
WSLFRPEKIPVADGERLMVTGKIPGLRVSGGDRLQISAVNDGMMTVIVPGRAEPASLPVGDSPFTALKLE
NGWVETPGHSVSDSAKVFASVTQMAMNNATLNGLARSGRDVRLYSSLDETRTAEKLSRHPSFTVVSEQIK
ARAGEVSLETAISRQKAGLHTPAQQAIHLALPVVESKNLAFSQVELLTEAKSFAAVGTGFADLGREIDAQ
IKRGDLLHVDVAKGYGTDLLISRASYDAEKSILHHVLEGKEAVTPLMERVSGELMETLTSGQRAATRMIL
ETKDRFTVVQGYAGVGKTTQFRAVMSAVNLLPEDERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDTQ
LQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAA
DVAIMKEIVRQTPELRDAVYSLINRDVNKALSGLENVKPIQVPRLKGAWVPENSVTEFSRLQERELVKAA
QEAEKKGEAFPDVPVTLYEAIVRDYTGRTPDAREQTLIVTHLNEDRRVLNSMTQDALAKPGEQQVTVPVL
TTANIRDGGLRRLSTWENHQGALALVDKVYHRIAGISKEDGLITLQDADGNTRLISPREAPAEGITLYNP
ETIRVGAGDRMRFTKSDRERGYVANSVWTVTAVSGDGVTLSDGKQTRVVRPGQDRAEQHIDLAYAITAHG
AQGASETFAIALEGTEGGRKQMAGFESAYVALSRMKQHVQVYTDDRQGWVKAINSAEQKGSAHDVLEPKS
EREMMNAERLFSTARELRDVAAGRAVLRNAGLAQGDSRARFIAPGRKYPQPYVALPAFDRNGKSAGIWLN
PLTTDDGAGLRGFSGEGRVKGSEEAQFVALQGSRNGESLLADNMQEGVRIARDNSDSGVVVRIAGDGRPW
NPGAITGGRVWGDIPDSSVQPGAGNGEPVTAEILAQRQAEEAVRRADEIVRKMAEDKSDLPEEKTAQAVR
EIAGQEQDRTLLPERKPPLPESVLREPVRERETILEVARESRVQERLQQMEQEMVRDLQKERTPGGD

Protein domains

Predicted by InterproScan.

• TrwC relaxase (TrwC) (11-282)

• AAA domain (AAA_30) (967-1158)

• single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N) (574-626)

• DNA helicase TraI (TraI_C) (1430-1553)

• DNA relaxase TraI 2B/2B-like domain (TraI_2B) (632-711)

Protein structure

No available structure.

Host bacterium

• ID: 1193
• GenBank: NZ_CP012345
• Plasmid name: pCFSAN000679_01
• Incompatibility group: IncFIB
• Plasmid size: 49584 bp
• Coordinate of oriT [Strand]: 102856..103114 [-]
• Host baterium: Salmonella enterica subsp. enterica serovar Choleraesuis str. ATCC 10708

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -