Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100732
Name   oriT_p34998-239.973kb in_silico
Organism   Enterobacter hormaechei subsp. steigerwaltii strain 34998
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP012168 (78000..78550 [+], 551 nt)
oriT length   551 nt
IRs (inverted repeats)      62..69, 72..79  (AACCCGTT..ACCGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 551 nt

>oriT_p34998-239.973kb
GAGCAGAGCTATGTGTGACAAGAAGTATAGAGATTATGAGGTAGCCATCATGGTCGATGTGAACCCGTTTGACCGGGTTATGAATGAGTTGAAAAGTCGTGGCCGCAAGAACGCTCATATCCTGAGCATCCTTCAATTCGACTGGCCCGCATCGGAGGTCATCATCGAGAAGCTGAGCTGTTACATCACAGATGGGATTAAGGCCAATCAGCAGCCTGTGATTTACCCGATCATTGAAGAAGCTCTGCATCGCTACAGCCAGCTCGTGTTTCATGAGCAGAGAGAAAAGTATGAGGACCCGGCCAGAATAGGGGCATTTCTGGAAACCCTGATCACTGAAACTTGCCGGGCGTTGGAAGTGCAAATTGTCGATAGTGGTGGCGATTCATGGTCTGTCGATTCAGGAGAGTCGTTCTCACTTTGGCTTTCTTCCCATCCAGGAGAACTATCCATTAACCCGCAGCCTCATGAGGATGAGACCTCTTTGCGTGGCTTGCTGTATGAGCTCATCACCTGTGAGAGTGTGAAAACTGTTTTAAGGAGAACCGACT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   804 GenBank   WP_000909947
Name   TraI_p34998-239.973kb insolico UniProt ID   _
Length   987 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 987 a.a.        Molecular weight: 109404.96 Da        Isoelectric Point: 4.8922

>WP_000909947.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALHKLFGGRSGAIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWATQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAYRHGIDRYFIRWRDKRHKRHEQFSL
LAWHRIIPAETQEFLSKSGPSIIEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVNEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISQDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGPVKILMLRLESNDLVFTTEPPAAVTGEVVGDVEDAEIE
FVDPEEADEDQAEGDAGLNNDMLAAELEAEKALAGLGFGDAMAMLKSTSDIAEEKPEQEDTESTELPKPD
ASKKGKQQSKPGKARAEKQPQKPEVKEDLSPQDIAKNAPPLANDDPLQALKDVGGGLGDIDFPFDVFNAS
AETACTDATNLEIPDITEPEKQEEQPKPDFVPQEQNSLQNGDFPIFSDSDEPPSWAIEPLPMLTDTPEQT
IPAPDMQHAGKPKELEKDAKTLLSEMLAGYGEASTLLEQAIMPVLEGKTTLGEVLCLMKGQAVILYPEGA
RSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLVDQLSDAVVAAIKDAEASMGGYQEAFELV
SPPGSGVSKNKSAPKQQSRKKTQQQKPEVDAGAGSEQKTKDKSLQQQPKEKQGNVASLVEEQKRKPVQDQ
EKQNVARLPKREAQPVAPKPKVEHERELGHVEVRERDEPEVREFEPPKAKTNPKDINEEDFLPSGVTPEK
ALLMLKDMIQKRSGRWLVTPVLEEDGCLVTSVKAFDMIAGENVGISKHILYGLLSRAQRRPLLKKRQGKL
YLEVDKA

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 58919..101238

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LI65_RS23570 (LI65_023765) 54148..54333 - 186 Protein_67 integrase core domain-containing protein -
LI65_RS23575 (LI65_023770) 54429..54959 - 531 WP_000043569 hypothetical protein -
LI65_RS23580 (LI65_023775) 55241..56146 - 906 WP_023093368 LysR family transcriptional regulator -
LI65_RS23585 (LI65_023780) 56452..58449 + 1998 WP_001163195 IncP-type conjugal transfer protein TraG -
LI65_RS23590 (LI65_023785) 58446..58922 + 477 WP_000085131 ribbon-helix-helix protein, CopG family -
LI65_RS23595 (LI65_023790) 58919..59989 + 1071 WP_023093367 P-type conjugative transfer ATPase TrbB virB11
LI65_RS23600 (LI65_023795) 59986..60372 + 387 WP_000150010 TrbC/VirB2 family protein virB2
LI65_RS23605 (LI65_023800) 60369..60638 + 270 WP_003092280 VirB3 family type IV secretion system protein virB3
LI65_RS23610 (LI65_023805) 60651..63101 + 2451 WP_000933135 conjugal transfer protein TrbE virb4
LI65_RS23615 (LI65_023810) 63098..63841 + 744 WP_003092277 P-type conjugative transfer protein TrbJ virB5
LI65_RS23620 (LI65_023815) 63854..64180 + 327 WP_014603538 hypothetical protein -
LI65_RS23625 (LI65_023820) 64177..65544 + 1368 WP_001000746 P-type conjugative transfer protein TrbL virB6
LI65_RS23630 (LI65_023825) 65563..66267 + 705 WP_001211312 conjugal transfer protein TrbF virB8
LI65_RS23635 (LI65_023830) 66264..67256 + 993 WP_000776560 P-type conjugative transfer protein TrbG virB9
LI65_RS23640 (LI65_023835) 67259..68539 + 1281 WP_003092274 TrbI/VirB10 family protein virB10
LI65_RS23645 (LI65_023840) 68536..68781 + 246 WP_000108283 DUF2274 domain-containing protein -
LI65_RS23650 (LI65_023845) 69119..69535 + 417 WP_012766321 hypothetical protein -
LI65_RS23655 (LI65_023850) 69573..69974 - 402 WP_039272428 hypothetical protein -
LI65_RS23660 (LI65_023855) 69985..72174 - 2190 WP_039272426 DNA topoisomerase 3 -
LI65_RS23665 (LI65_023860) 72174..72407 - 234 WP_039272424 hypothetical protein -
LI65_RS23670 (LI65_023865) 72389..73006 - 618 WP_012766323 hypothetical protein -
LI65_RS23675 (LI65_023870) 73174..76137 + 2964 WP_000909947 MobH family relaxase -
LI65_RS23680 (LI65_023875) 76134..77999 + 1866 WP_000178858 conjugative transfer system coupling protein TraD virb4
LI65_RS23685 (LI65_023880) 78049..78594 + 546 WP_023356350 hypothetical protein -
LI65_RS23690 (LI65_023885) 78551..79180 + 630 WP_000743448 DUF4400 domain-containing protein tfc7
LI65_RS23695 (LI65_023890) 79191..79637 + 447 WP_039272421 hypothetical protein -
LI65_RS23700 (LI65_023895) 79647..80024 + 378 WP_000869260 hypothetical protein -
LI65_RS23705 (LI65_023900) 80024..80686 + 663 WP_039272419 hypothetical protein -
LI65_RS27980 80865..81008 + 144 WP_168199038 hypothetical protein -
LI65_RS23710 (LI65_023905) 81021..81368 + 348 WP_039272417 hypothetical protein -
LI65_RS23715 (LI65_023910) 81511..81792 + 282 WP_000805626 type IV conjugative transfer system protein TraL traL
LI65_RS23720 (LI65_023915) 81789..82415 + 627 WP_039272415 TraE/TraK family type IV conjugative transfer system protein traE
LI65_RS23725 (LI65_023920) 82399..83316 + 918 WP_012766326 type-F conjugative transfer system secretin TraK traK
LI65_RS23730 (LI65_023925) 83313..84629 + 1317 WP_039272412 TraB/VirB10 family protein traB
LI65_RS23735 (LI65_023930) 84626..85204 + 579 WP_039272410 type IV conjugative transfer system lipoprotein TraV traV
LI65_RS23740 (LI65_023935) 85208..85600 + 393 WP_012766327 TraA family conjugative transfer protein -
LI65_RS23745 (LI65_023940) 85801..91287 + 5487 WP_039272407 Ig-like domain-containing protein -
LI65_RS23750 (LI65_023945) 91436..92143 + 708 WP_001259344 DsbC family protein -
LI65_RS23755 (LI65_023950) 92140..94587 + 2448 WP_051479468 type IV secretion system protein TraC virb4
LI65_RS23760 (LI65_023955) 94602..94919 + 318 WP_000364398 hypothetical protein -
LI65_RS23765 (LI65_023960) 94916..95446 + 531 WP_000672456 S26 family signal peptidase -
LI65_RS23770 (LI65_023965) 95409..96677 + 1269 WP_039272404 TrbC family F-type conjugative pilus assembly protein traW
LI65_RS23775 (LI65_023970) 96674..97351 + 678 WP_039272402 EAL domain-containing protein -
LI65_RS23780 (LI65_023975) 97345..98349 + 1005 WP_000911304 TraU family protein traU
LI65_RS23785 (LI65_023980) 98500..101238 + 2739 WP_039272401 conjugal transfer mating pair stabilization protein TraN traN
LI65_RS23790 (LI65_023985) 101277..102137 - 861 WP_000679674 hypothetical protein -
LI65_RS23795 (LI65_023990) 102249..102899 - 651 WP_000796641 hypothetical protein -
LI65_RS23800 (LI65_023995) 103191..103511 + 321 WP_012766334 hypothetical protein -
LI65_RS23805 (LI65_024000) 103822..104007 + 186 WP_001186892 hypothetical protein -
LI65_RS23810 (LI65_024005) 104228..105196 + 969 WP_001553257 AAA family ATPase -
LI65_RS23815 (LI65_024010) 105208..106113 + 906 WP_039272396 hypothetical protein -

Host bacterium

ID   1192 GenBank   NZ_CP012168
Plasmid name   p34998-239.973kb Incompatibility group   IncA/C
Plasmid size   239973 bp Coordinate of oriT [Strand]   78000..78550 [+]
Host baterium   Enterobacter hormaechei subsp. steigerwaltii strain 34998

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   mercury resistance
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -