Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100725 |
| Name | oriT_pCAV1374-84  |
| Organism | Klebsiella oxytoca strain CAV1374 |
| Sequence Completeness | core |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP011632 (9434..9538 [+], 105 nt) |
| oriT length | 105 nt |
| IRs (inverted repeats) | 18..35, 42..59 (GTTTTTGGTACACCGCCG..CGGCAGTGACGCAAAAAC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 105 nt
>oriT_pCAV1374-84
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 797 | GenBank | WP_004206924 |
| Name | PutativerelaxaseofpCAV1374-84 |
UniProt ID | A0A0G3RZP6 |
| Length | 659 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75087.84 Da Isoelectric Point: 10.0285
>WP_004206924.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0G3RZP6 |
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 12336..31532
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AB185_RS01055 (AB185_01055) | 7420..7731 | + | 312 | WP_004187333 | hypothetical protein | - |
| AB185_RS01060 (AB185_01060) | 7864..8400 | + | 537 | WP_004206920 | hypothetical protein | - |
| AB185_RS01065 (AB185_01065) | 8901..9266 | - | 366 | WP_004206922 | hypothetical protein | - |
| AB185_RS01070 (AB185_01070) | 9542..9859 | + | 318 | WP_004206923 | plasmid mobilization protein MobA | - |
| AB185_RS01075 (AB185_01075) | 9846..11825 | + | 1980 | WP_004206924 | TraI/MobA(P) family conjugative relaxase | - |
| AB185_RS01080 (AB185_01080) | 11839..12339 | + | 501 | WP_004206925 | DotD/TraH family lipoprotein | - |
| AB185_RS01085 (AB185_01085) | 12336..13115 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| AB185_RS01090 (AB185_01090) | 13126..14289 | + | 1164 | WP_004206926 | plasmid transfer ATPase TraJ | virB11 |
| AB185_RS01095 (AB185_01095) | 14279..14539 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| AB185_RS38710 | 14564..17869 | + | 3306 | WP_219818010 | LPD7 domain-containing protein | - |
| AB185_RS34640 | 17835..18347 | + | 513 | WP_011091071 | hypothetical protein | traL |
| AB185_RS34890 | 18348..18545 | - | 198 | WP_004187464 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| AB185_RS01110 (AB185_01110) | 18532..18942 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| AB185_RS01115 (AB185_01115) | 18941..19723 | + | 783 | WP_004206929 | DotI/IcmL family type IV secretion protein | traM |
| AB185_RS34895 (AB185_01125) | 19732..20883 | + | 1152 | WP_015062843 | DotH/IcmK family type IV secretion protein | traN |
| AB185_RS01130 (AB185_01130) | 20895..22244 | + | 1350 | WP_004206932 | conjugal transfer protein TraO | traO |
| AB185_RS01135 (AB185_01135) | 22256..22960 | + | 705 | WP_004206933 | conjugal transfer protein TraP | traP |
| AB185_RS01140 (AB185_01140) | 22984..23514 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| AB185_RS01145 (AB185_01145) | 23531..23920 | + | 390 | WP_011154472 | DUF6750 family protein | traR |
| AB185_RS01150 (AB185_01150) | 23966..24460 | + | 495 | WP_004187480 | hypothetical protein | - |
| AB185_RS01155 (AB185_01155) | 24457..27507 | + | 3051 | WP_047722547 | conjugal transfer protein | traU |
| AB185_RS01160 (AB185_01160) | 27504..28712 | + | 1209 | WP_004187486 | conjugal transfer protein TraW | traW |
| AB185_RS01165 (AB185_01165) | 28709..29359 | + | 651 | WP_004187488 | hypothetical protein | - |
| AB185_RS01170 (AB185_01170) | 29352..31532 | + | 2181 | WP_004187492 | DotA/TraY family protein | traY |
| AB185_RS01175 (AB185_01175) | 31535..32188 | + | 654 | WP_004206935 | hypothetical protein | - |
| AB185_RS01180 (AB185_01180) | 32262..32492 | + | 231 | WP_004187496 | IncL/M type plasmid replication protein RepC | - |
| AB185_RS01185 (AB185_01185) | 32777..33844 | + | 1068 | WP_020316718 | plasmid replication initiator RepA | - |
Host bacterium
| ID | 1185 | GenBank | NZ_CP011632 |
| Plasmid name | pCAV1374-84 | Incompatibility group | IncL/M |
| Plasmid size | 83652 bp | Coordinate of oriT [Strand] | 9434..9538 [+] |
| Host baterium | Klebsiella oxytoca strain CAV1374 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | mercury resistance |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |