Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100724
Name   oriT_pKPC_CAV1344 in_silico
Organism   Klebsiella pneumoniae strain CAV1344
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP011622 (18319..18869 [-], 551 nt)
oriT length   551 nt
IRs (inverted repeats)      IR1: 62..70, 71..79  (AACCCTTTC..GACAGGGTT)
  IR2: 143..150, 154..161  (TGGCCTGC..GGAGGCCA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 551 nt

>oriT_pKPC_CAV1344
GAGCAGAGCTATGTGTGACAAGAAGTATAGAAATTACGAGGTAGCCATCATGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAAGGCTAATCAGGAGCCTGTGATTTACCCGATCATTGAAGAAGCTCTGCATCGCTACAGCCAGCTCGTGTTTCATGAGCAGAGAGAGAAATATGAAGACCCGGCCAGAATTGGGGCATTTCTGGAAACCCTGATCACCGAAACCTGCCGGGCGTTGGAAGTGCAAATTGTCGATAGTGGCGGTGATTCATGGTCTGTCGATTCAGGAGAGTCGTTCTCACTGTGGCTTTCTTCCCATCCAGGAGAACTATCCATTAACCCGCAGCCCCATGAGGATGAGACCTCTTTGCGTGGCTTGCTGTATGAGCTCATCACCTGTGAGAGCGTGAAAACTGTTTTAAGGAGAACCGACT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   796 GenBank   WP_001326173
Name   TraI_pKPC_CAV1344 insolico UniProt ID   _
Length   990 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 990 a.a.        Molecular weight: 109356.90 Da        Isoelectric Point: 4.9395

>WP_001326173.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 175..20735

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AB186_RS00700 (AB186_00700) 175..1440 - 1266 WP_001447719 TrbC family F-type conjugative pilus assembly protein traW
AB186_RS00705 (AB186_00705) 1403..1933 - 531 WP_001010740 S26 family signal peptidase -
AB186_RS00710 (AB186_00710) 1930..2247 - 318 WP_000351984 hypothetical protein -
AB186_RS00715 (AB186_00715) 2262..4709 - 2448 WP_000637384 type IV secretion system protein TraC virb4
AB186_RS00720 (AB186_00720) 4706..5413 - 708 WP_001259346 DsbC family protein -
AB186_RS00725 (AB186_00725) 5562..11048 - 5487 WP_047718077 Ig-like domain-containing protein -
AB186_RS00730 (AB186_00730) 11249..11641 - 393 WP_000479535 TraA family conjugative transfer protein -
AB186_RS00735 (AB186_00735) 11645..12223 - 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
AB186_RS00740 (AB186_00740) 12220..13533 - 1314 WP_024131605 TraB/VirB10 family protein traB
AB186_RS00745 (AB186_00745) 13533..14450 - 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
AB186_RS00750 (AB186_00750) 14434..15060 - 627 WP_001049717 TraE/TraK family type IV conjugative transfer system protein traE
AB186_RS00755 (AB186_00755) 15057..15338 - 282 WP_000805625 type IV conjugative transfer system protein TraL traL
AB186_RS00760 (AB186_00760) 15483..15848 - 366 WP_001052530 hypothetical protein -
AB186_RS00765 (AB186_00765) 16184..16846 - 663 WP_001231464 hypothetical protein -
AB186_RS00770 (AB186_00770) 16846..17223 - 378 WP_000869297 hypothetical protein -
AB186_RS00775 (AB186_00775) 17233..17679 - 447 WP_000122507 hypothetical protein -
AB186_RS00780 (AB186_00780) 17689..18318 - 630 WP_000743449 DUF4400 domain-containing protein tfc7
AB186_RS00785 (AB186_00785) 18275..18820 - 546 WP_000228720 hypothetical protein -
AB186_RS00790 (AB186_00790) 18870..20735 - 1866 WP_000178857 conjugative transfer system coupling protein TraD virb4
AB186_RS00795 (AB186_00795) 20732..23704 - 2973 WP_001326173 MobH family relaxase -
AB186_RS00800 (AB186_00800) 23872..24489 + 618 WP_001249395 hypothetical protein -
AB186_RS00805 (AB186_00805) 24471..24704 + 234 WP_001191890 hypothetical protein -

Host bacterium

ID   1184 GenBank   NZ_CP011622
Plasmid name   pKPC_CAV1344 Incompatibility group   IncA/C2
Plasmid size   176497 bp Coordinate of oriT [Strand]   18319..18869 [-]
Host baterium   Klebsiella pneumoniae strain CAV1344

Cargo genes

Drug resistance gene   ant(2'')-Ia, qacE, sul1, qnrA1, blaKPC-2, aac(6')-Ib, ant(3'')-Ia, blaOXA-9, blaTEM-1A, blaSHV-30
Virulence gene   -
Metal resistance gene   merR, merT, merP, merC, merA, merD, merE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -