Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100722
Name   oriT_pCAV1335-5410 in_silico
Organism   Klebsiella oxytoca strain CAV1335
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP011613 (3018..3192 [-], 175 nt)
oriT length   175 nt
IRs (inverted repeats)      2..11, 15..24  (GTTTCGGGGC..GCCCTGAACC)
Location of nic site      55..56
Conserved sequence flanking the
  nic site    		  CTGG|CTTA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 175 nt

>oriT_pCAV1335-5410
GGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTACTATGTTGGCACGGATGAGGGTGTCAGTGAAGTGCTTCATGTGGCAGGAGAAAAAAGGCGGCACCGGTGCGTCAGCAGAATATGTGATACAGGATATATTCCGCTTCCTCGCTCAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   794 GenBank   WP_047719615
Name   PutativerelaxaseofpCAV1335-5410 insolico UniProt ID   _
Length   498 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 498 a.a.        Molecular weight: 55043.39 Da        Isoelectric Point: 9.0463

>WP_047719615.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacteriaceae]
MIVKFHPRGRGGGAGPVDYLLGKDRQREGATVLQGKPEEVRELIDASPYVKKYTSGVLSFAEADLPPGQR
EKLMASFERVLMPGLDKDQYSILWVEHADKGRLELNFLIPNTELLTGRRLQPYYDRADRPRIDAWQTIVN
GRLGLHDPNAPENRRALVTPSGLPKTKQEAAEAITRGLLTLASSGELKSRQDVTDTLEGAGFEVVRTTKS
SISIADPDGGRNIRLKGAIYEQSFNAGEGLRAAIESAAAEYRRDAESRIQRARAVCQSGTERKREENQRR
HPRPRAEYERGDGEKPAERTADCRADMAHHPDGVSAADRHEREHSVVAGAADNRQLHASSSAGRYPGEND
RPDVGSALSGEQRRPEVSYPAAGDTNRSHPLRRDDMDPPETGVAENDRAGKAVAERIRAATAGLLAKAGR
MGERLRGMAEHVWAYATGERGAERARVGLEHAGAEFKRAAAPLVRELNAIELRQRENEYQKTLELERQPQ
KTYRGPTL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(57-238)


  Protein structure



No available structure.




Host bacterium

ID   1182 GenBank   NZ_CP011613
Plasmid name   pCAV1335-5410 Incompatibility group   ColRNAI
Plasmid size   5410 bp Coordinate of oriT [Strand]   3018..3192 [-]
Host baterium   Klebsiella oxytoca strain CAV1335

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -