Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100720
Name   oriT_pCAV1321-3820 in_silico
Organism   Citrobacter freundii strain CAV1321
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP011605 (937..1110 [-], 174 nt)
oriT length   174 nt
IRs (inverted repeats)      2..11, 15..24  (GTTTCGGGGC..GCCCTGAACC)
Location of nic site      55..56
Conserved sequence flanking the
  nic site    		  CTGG|CTTA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 174 nt

>oriT_pCAV1321-3820
GGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTACTATGTTGGCACGGATGCGAGTGTCAGTGAAGTGCTTCATGTGGCAGGAGAAAAATGCAGCAGCGGAGCGTCAGCAGAATATGTGATACAGGATATATTCCGCTTCCTCGCTCAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   792 GenBank   WP_023063529
Name   PutativerelaxaseofpCAV1321-3820 insolico UniProt ID   _
Length   501 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 501 a.a.        Molecular weight: 55268.41 Da        Isoelectric Point: 7.1149

>WP_023063529.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacteriaceae]
MIVKFHPRGRGGGAGPVDYLLGKDRQRDGASILQGKPEEVRELIDASPYAKKYTSGVLSFAEQDLPPGQR
EKLMASFERVLMPGLDKDQYSVLWVEHQDKGRLELNFLIPNTELLTGKRLQPYYDRADRPRIDAWQTIVN
GRLGLHDPNAPENRRALVTPSALPETKQEAAQAITRGLLSLASSGELKTRQDVTKALESAGFEVVRTTKS
SISIADPDGGRNIRLKGAIYEQSFNAGEGLRAEIESAAADYRRDAESRIQRAREVCQSGTERKREENQRR
HPRPRPEAVLSHEPAHERDAAHGQPDVADHRPGVHAADSLERGHSVVAGATDTRQLHEYPGAERRAGEAE
RQDVGREVPGRQPGAFSGAAGGRESGHELDVRQRQEEGHQTGTGVEQHDGAGKTVIERIRAATTGLLEKA
GRVGECLRGMADDVWTYATGERGAERARHGLEQAGAEFERAAAPVVEQLNTIEHRQQHERSQVQKILELE
KAPRARYGPTL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(57-238)


  Protein structure



No available structure.




Host bacterium

ID   1180 GenBank   NZ_CP011605
Plasmid name   pCAV1321-3820 Incompatibility group   ColRNAI
Plasmid size   3820 bp Coordinate of oriT [Strand]   937..1110 [-]
Host baterium   Citrobacter freundii strain CAV1321

Cargo genes

Drug resistance gene   -
Virulence gene   virulence factor
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -