Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100718 |
| Name | oriT_pCAV1151-83  |
| Organism | Phytobacter ursingii strain CAV1151 |
| Sequence Completeness | core |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP011599 (9116..9220 [+], 105 nt) |
| oriT length | 105 nt |
| IRs (inverted repeats) | 18..35, 42..59 (GTTTTTGGTACACCGCCG..CGGCAGTGACGCAAAAAC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 105 nt
>oriT_pCAV1151-83
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 790 | GenBank | WP_004206924 |
| Name | PutativerelaxaseofpCAV1151-83 |
UniProt ID | A0A0G3Q6S1 |
| Length | 659 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75087.84 Da Isoelectric Point: 10.0285
>WP_004206924.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0G3Q6S1 |
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 12018..31214
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AB182_RS00330 (AB182_00325) | 7102..7413 | + | 312 | WP_004187333 | hypothetical protein | - |
| AB182_RS00335 (AB182_00330) | 7546..8082 | + | 537 | WP_004206920 | hypothetical protein | - |
| AB182_RS00340 (AB182_00335) | 8583..8948 | - | 366 | WP_004206922 | hypothetical protein | - |
| AB182_RS00345 (AB182_00340) | 9224..9541 | + | 318 | WP_004206923 | plasmid mobilization protein MobA | - |
| AB182_RS00350 (AB182_00345) | 9528..11507 | + | 1980 | WP_004206924 | TraI/MobA(P) family conjugative relaxase | - |
| AB182_RS00355 (AB182_00350) | 11521..12021 | + | 501 | WP_004206925 | DotD/TraH family lipoprotein | - |
| AB182_RS00360 (AB182_00355) | 12018..12797 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| AB182_RS00365 (AB182_00360) | 12808..13971 | + | 1164 | WP_004206926 | plasmid transfer ATPase TraJ | virB11 |
| AB182_RS00370 (AB182_00365) | 13961..14221 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| AB182_RS32805 | 14246..17551 | + | 3306 | WP_219818010 | LPD7 domain-containing protein | - |
| AB182_RS29675 | 17517..18029 | + | 513 | WP_011091071 | hypothetical protein | traL |
| AB182_RS00385 (AB182_00380) | 18030..18242 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| AB182_RS00390 (AB182_00385) | 18214..18624 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| AB182_RS00395 (AB182_00390) | 18692..19405 | + | 714 | WP_223294958 | DotI/IcmL family type IV secretion protein | traM |
| AB182_RS00400 (AB182_00395) | 19414..20565 | + | 1152 | WP_015062843 | DotH/IcmK family type IV secretion protein | traN |
| AB182_RS00405 (AB182_00400) | 20577..21926 | + | 1350 | WP_004206932 | conjugal transfer protein TraO | traO |
| AB182_RS00410 (AB182_00405) | 21938..22642 | + | 705 | WP_004206933 | conjugal transfer protein TraP | traP |
| AB182_RS00415 (AB182_00410) | 22666..23196 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| AB182_RS00420 (AB182_00415) | 23213..23602 | + | 390 | WP_011154472 | DUF6750 family protein | traR |
| AB182_RS00425 (AB182_00420) | 23648..24142 | + | 495 | WP_004187480 | hypothetical protein | - |
| AB182_RS00430 (AB182_00425) | 24139..27189 | + | 3051 | WP_011154474 | conjugative transfer protein | traU |
| AB182_RS00435 (AB182_00430) | 27186..28394 | + | 1209 | WP_004187486 | conjugal transfer protein TraW | traW |
| AB182_RS00440 (AB182_00435) | 28391..29041 | + | 651 | WP_004187488 | hypothetical protein | - |
| AB182_RS00445 (AB182_00440) | 29034..31214 | + | 2181 | WP_004187492 | DotA/TraY family protein | traY |
| AB182_RS00450 (AB182_00445) | 31217..31870 | + | 654 | WP_004206935 | hypothetical protein | - |
| AB182_RS00455 (AB182_00450) | 31944..32174 | + | 231 | WP_004187496 | IncL/M type plasmid replication protein RepC | - |
| AB182_RS00460 (AB182_00455) | 32471..33526 | + | 1056 | WP_015062846 | plasmid replication initiator RepA | - |
| AB182_RS00465 (AB182_00460) | 34698..35231 | - | 534 | Protein_41 | IS110-like element IS4321 family transposase | - |
Host bacterium
| ID | 1178 | GenBank | NZ_CP011599 |
| Plasmid name | pCAV1151-83 | Incompatibility group | IncL/M |
| Plasmid size | 82986 bp | Coordinate of oriT [Strand] | 9116..9220 [+] |
| Host baterium | Phytobacter ursingii strain CAV1151 |
Cargo genes
| Drug resistance gene | ant(2'')-Ia, blaOXA-2, qacE, sul1, blaSHV-30 |
| Virulence gene | - |
| Metal resistance gene | merE, merD, merA, merC, merP, merT, merR |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |