Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100714 |
| Name | oriT_pKPC_CAV1043  |
| Organism | Enterobacter asburiae strain CAV1043 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP011589 (45730..46356 [+], 627 nt) |
| oriT length | 627 nt |
| IRs (inverted repeats) | IR1: 198..207, 211..220 (ATTGGGTGTT..AATACCTAAT) IR2:319..324, 330..335 (AAAAAA..TTTTTT) IR3: 393..402, 405..414 (GTATTCATGC..GCATGAATAC) |
| Location of nic site | 301..302 |
| Conserved sequence flanking the nic site |
GGTGT|ATAGC |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 627 nt
>oriT_pKPC_CAV1043
AGCGCCGCAGATAATCTGACCGATTACCTCCTGAAACCAGGTCTATATAGGCCAAAAGTTCATCTGATACTTTTGCGGTTATTATTGGCATTCAGTCCTCACATTGTGCATTTTTTAAACAAAAAATTGGGATCTAACAAGCTGAAATCTTAGTATTACCAAAGTAATAAAGCAAACTCATTATAAAACAATGAGTTATTGGGTGTTTTTAATACCTAATTATTACCGAATATTGTTGCTATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAGGGGCGATCTACGTAGGTTAAGGACTAACTGGCTAAAAAGCGTTCAATATTCCGTATTCATGCTTGCATGAATACCAGTACAACAAAAGTACATCAAAATTACATCAAAATTACATCACTTGAAGGTTGACAGTACAACAAAATTACATCATTCTTTGGTCATGAGGTAGCCAGTACAACAAAAGTACATCAAAAGTACATCAAAATTACATCAAAATTACATCATTCTAAATGAGGGTACTATGAAGCCCAAAAGTATCAGGGCGGCACTTCAGTTGATGTTGCCGG
AGCGCCGCAGATAATCTGACCGATTACCTCCTGAAACCAGGTCTATATAGGCCAAAAGTTCATCTGATACTTTTGCGGTTATTATTGGCATTCAGTCCTCACATTGTGCATTTTTTAAACAAAAAATTGGGATCTAACAAGCTGAAATCTTAGTATTACCAAAGTAATAAAGCAAACTCATTATAAAACAATGAGTTATTGGGTGTTTTTAATACCTAATTATTACCGAATATTGTTGCTATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAGGGGCGATCTACGTAGGTTAAGGACTAACTGGCTAAAAAGCGTTCAATATTCCGTATTCATGCTTGCATGAATACCAGTACAACAAAAGTACATCAAAATTACATCAAAATTACATCACTTGAAGGTTGACAGTACAACAAAATTACATCATTCTTTGGTCATGAGGTAGCCAGTACAACAAAAGTACATCAAAAGTACATCAAAATTACATCAAAATTACATCATTCTAAATGAGGGTACTATGAAGCCCAAAAGTATCAGGGCGGCACTTCAGTTGATGTTGCCGG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 786 | GenBank | WP_001144737 |
| Name | MobC_pKPC_CAV1043 |
UniProt ID | _ |
| Length | 88 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 88 a.a. Molecular weight: 10315.57 Da Isoelectric Point: 5.5791
>WP_001144737.1 MULTISPECIES: plasmid mobilization relaxosome protein MobC [Gammaproteobacteria]
MPVLTDEEVNTVRESCRQLGAIGRNLNQVARALNIEFRESDKLKQEAIEKLAERIDQHLDHVSELFDKTW
SRWHDCHFQKTTAPVDWA
MPVLTDEEVNTVRESCRQLGAIGRNLNQVARALNIEFRESDKLKQEAIEKLAERIDQHLDHVSELFDKTW
SRWHDCHFQKTTAPVDWA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 27802..45402
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AB190_RS00835 (AB190_00825) | 23606..25717 | + | 2112 | Protein_24 | Tn3-like element Tn3 family transposase | - |
| AB190_RS24710 | 25737..26297 | - | 561 | Protein_25 | IS6-like element IS26 family transposase | - |
| AB190_RS24715 (AB190_00835) | 26362..26541 | - | 180 | Protein_26 | helix-turn-helix domain-containing protein | - |
| AB190_RS26730 (AB190_00840) | 26808..27095 | + | 288 | Protein_27 | DUF6710 family protein | - |
| AB190_RS00855 (AB190_00845) | 27269..27802 | - | 534 | WP_000792636 | phospholipase D family protein | - |
| AB190_RS00860 (AB190_00850) | 27802..28797 | - | 996 | WP_000128596 | ATPase, T2SS/T4P/T4SS family | virB11 |
| AB190_RS00865 (AB190_00855) | 28839..29999 | - | 1161 | WP_000101710 | type IV secretion system protein VirB10 | virB10 |
| AB190_RS00870 (AB190_00860) | 29999..30883 | - | 885 | WP_000735066 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| AB190_RS00875 (AB190_00865) | 30894..31592 | - | 699 | WP_000646594 | type IV secretion system protein | virB8 |
| AB190_RS26735 | 31582..31740 | - | 159 | WP_012561180 | hypothetical protein | - |
| AB190_RS00880 (AB190_00870) | 31811..32851 | - | 1041 | WP_001749958 | type IV secretion system protein | virB6 |
| AB190_RS00885 (AB190_00875) | 32867..33094 | - | 228 | WP_001749959 | IncN-type entry exclusion lipoprotein EexN | - |
| AB190_RS00890 (AB190_00880) | 33102..33815 | - | 714 | WP_001749960 | type IV secretion system protein | virB5 |
| AB190_RS00895 (AB190_00885) | 33833..36301 | - | 2469 | WP_224743086 | VirB4 family type IV secretion/conjugal transfer ATPase | virb4 |
| AB190_RS00900 (AB190_00890) | 36433..36750 | - | 318 | WP_000496058 | VirB3 family type IV secretion system protein | virB3 |
| AB190_RS00905 (AB190_00895) | 36800..37093 | - | 294 | WP_001749962 | hypothetical protein | virB2 |
| AB190_RS00910 (AB190_00900) | 37103..37384 | - | 282 | WP_000440698 | transcriptional repressor KorA | - |
| AB190_RS00915 (AB190_00905) | 37393..38127 | - | 735 | WP_001749963 | lytic transglycosylase domain-containing protein | virB1 |
| AB190_RS00920 (AB190_00910) | 38170..38541 | + | 372 | WP_011867773 | H-NS family nucleoid-associated regulatory protein | - |
| AB190_RS00925 (AB190_00915) | 38557..38901 | + | 345 | WP_001749964 | hypothetical protein | - |
| AB190_RS00930 (AB190_00920) | 38995..39699 | - | 705 | WP_001067855 | IS6-like element IS26 family transposase | - |
| AB190_RS00935 (AB190_00925) | 39765..40262 | + | 498 | Protein_45 | Tn3 family transposase | - |
| AB190_RS00940 (AB190_00930) | 40272..40637 | - | 366 | WP_012561167 | DUF6710 family protein | - |
| AB190_RS00945 (AB190_00935) | 40637..43873 | - | 3237 | WP_012561166 | MobF family relaxase | - |
| AB190_RS00950 (AB190_00940) | 43873..45402 | - | 1530 | WP_000342688 | type IV secretion system DNA-binding domain-containing protein | virb4 |
| AB190_RS00955 (AB190_00945) | 45404..45820 | - | 417 | WP_001749975 | hypothetical protein | - |
| AB190_RS26545 (AB190_00950) | 46311..46730 | + | 420 | WP_001749974 | plasmid stabilization protein StbA | - |
| AB190_RS00965 (AB190_00955) | 46739..47455 | + | 717 | WP_001749973 | StbB family protein | - |
| AB190_RS00970 (AB190_00960) | 47457..47825 | + | 369 | WP_000414913 | plasmid stabilization protein StbC | - |
| AB190_RS00975 (AB190_00965) | 48007..48351 | + | 345 | WP_000999874 | hypothetical protein | - |
| AB190_RS00980 (AB190_00970) | 48462..48782 | - | 321 | WP_001749971 | protein CcgAII | - |
| AB190_RS00985 (AB190_00975) | 48837..49016 | - | 180 | WP_000214483 | protein CcgAI | - |
| AB190_RS00990 (AB190_00980) | 49743..50252 | - | 510 | WP_000129958 | antirestriction protein ArdA | - |
Host bacterium
| ID | 1174 | GenBank | NZ_CP011589 |
| Plasmid name | pKPC_CAV1043 | Incompatibility group | IncN |
| Plasmid size | 59138 bp | Coordinate of oriT [Strand] | 45730..46356 [+] |
| Host baterium | Enterobacter asburiae strain CAV1043 |
Cargo genes
| Drug resistance gene | dfrA14, blaKPC-3 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |