Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100713
Name   oriT_pCAV1311-3223 in_silico
Organism   Enterobacter hormaechei strain CAV1311
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP011569 (1500..1692 [-], 193 nt)
oriT length   193 nt
IRs (inverted repeats)      22..29, 33..40  (TTCGGGGC..GCCCTGAA)
Location of nic site      73..74
Conserved sequence flanking the
  nic site    		  CTGG|CTAA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 193 nt

>oriT_pCAV1311-3223
GCGGCAGTGGTTTTAGCGGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTAAACATGTTATGACGGAGTGGTAATTTATGAAAGTGCTTCATGTGACAGGGGAAAAATGCGGCTGCCGGGCGTAAGTGCAAAATGCAGGTGGTGATGAAATGACGCCTCCTCGCTCAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   785 GenBank   WP_045265544
Name   PutativerelaxaseofpCAV1311-3223 insolico UniProt ID   A0A142BR45
Length   497 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 497 a.a.        Molecular weight: 54828.84 Da        Isoelectric Point: 6.7956

>WP_045265544.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacterales]
MIVKFHPRGRGGGGGPVDYLLGKDRQRDGASVLQGKPDEVRELIDASPYAKKYTSGVLSFAEQDLPPGQR
EKLMASFERVLMPGLDKDQYSVLWVEHRDKGRLELNFLIPNTELLTGKRLQPYYDRADRPRIDAWQTIVN
GRLGLHDPNAPENRRVLVSPSALPEAKQEAAQAITSGLLALASSGELKTRQDVTEALESAGFEVVRTTKS
SISIADPDGGRNIRLKGAIYEQSFNAGEGLRAEIESAAADYRRDAESRIQRAREVCQSGTERKREENQRR
HPRPRPEAVLSHEPAHERDAAHGQPDVADHRSGLRAADSVERGHSVVAGAADTRELYDHPGAEEHAGHAV
REEQRRTALDLRRAETPLREGEPGSGTVRRGLELDDTGGEIAHDGAGKTVAERIRAATAGLLEKAGRVGE
RLRGMADDVWSYSTGERGAERARHGLEQAGAEFERAAAPVVERLNAIETHRQQERAVQHQKALELERSQW
QHHGPSL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(57-238)


  Protein structure


Source ID Structure
AlphaFold DB A0A142BR45


Host bacterium

ID   1173 GenBank   NZ_CP011569
Plasmid name   pCAV1311-3223 Incompatibility group   ColRNAI
Plasmid size   3223 bp Coordinate of oriT [Strand]   1500..1692 [-]
Host baterium   Enterobacter hormaechei strain CAV1311

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -