Detailed information of oriT
oriT
The information of the oriT region
oriTDB ID | 100712 |
Name | oriT_pNCM3722-F |
Organism | Escherichia coli strain NCM3722 isolate K-12 |
Sequence Completeness | intact |
NCBI accession of oriT (coordinates [strand]) | NZ_CP011496 (2243..2532 [-], 290 nt) |
oriT length | 290 nt |
IRs (inverted repeats) | 225..232, 235..242 n (GCAAAAAC..GTTTTTGC) |
Location of nic site | 251..252 |
Conserved sequence flanking the nic site |
GTGGGGTGT|GG |
Note | predicted by the oriTfinder |
oriT sequence
Download Length: 290 nt
>oriT_pNCM3722-F
CGCACCGCTAGCAGCGCCCCTAGCGGTATCCTATAAAAAAACACACCGCGCCGCTAGCAGCACCCCTAATATAAAATAATGTTTTTTATAAAAATAGTCAGTACCACCCCTACAAAACGGTGTCGGCGCGTTGTTGTAGCCGCGCCGACACCGCTTTTTTAAATATCATAAAGAGAGTAAGAGAAACTAATTTTTCATAACACTCTATTTATAAAGAAAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCT
CGCACCGCTAGCAGCGCCCCTAGCGGTATCCTATAAAAAAACACACCGCGCCGCTAGCAGCACCCCTAATATAAAATAATGTTTTTTATAAAAATAGTCAGTACCACCCCTACAAAACGGTGTCGGCGCGTTGTTGTAGCCGCGCCGACACCGCTTTTTTAAATATCATAAAGAGAGTAAGAGAAACTAATTTTTCATAACACTCTATTTATAAAGAAAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure fileRelaxase
ID | 784 | GenBank | WP_000987005 |
Name | TraI_pNCM3722-F | UniProt ID | _ |
Length | 1756 a.a. | PDB ID | |
Note | putative relaxase |
Relaxase protein sequence
Download Length: 1756 a.a. Molecular weight: 192015.91 Da Isoelectric Point: 5.8951
>WP_000987005.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]
MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEIKMAEWMQT
LKETGFDIRAYRDAADQRADLRTLTPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNMKQDERLSGELITGR
RQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGLPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVSDSPFTALKL
ENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KTRAGETSLETAISHQKSALHTPAQQAIHLALPVVESKKLAFSMVDLLTEAKSFAAEGTGFTELGGEINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVMPLMERVPGELMEKLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEA
QQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDVREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWETHRDALVLVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVT
LYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTREIRPGQEQAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVF
EPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGKTEQAVREIAGQERDRAAITEREAALPEGVLREPQRVREAVREIARENLLQERLQQMERDMVRDLQKE
KTLGGD
MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEIKMAEWMQT
LKETGFDIRAYRDAADQRADLRTLTPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNMKQDERLSGELITGR
RQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGLPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVSDSPFTALKL
ENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KTRAGETSLETAISHQKSALHTPAQQAIHLALPVVESKKLAFSMVDLLTEAKSFAAEGTGFTELGGEINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVMPLMERVPGELMEKLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEA
QQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDVREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWETHRDALVLVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVT
LYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTREIRPGQEQAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVF
EPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGKTEQAVREIAGQERDRAAITEREAALPEGVLREPQRVREAVREIARENLLQERLQQMERDMVRDLQKE
KTLGGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1681..28082
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
AA953_RS27195 (AA953_23295) | 14..133 | + | 120 | WP_014106970 | hypothetical protein | - |
AA953_RS23235 (AA953_23300) | 158..445 | + | 288 | WP_000107542 | hypothetical protein | - |
AA953_RS23240 (AA953_23305) | 564..1385 | + | 822 | WP_001234417 | DUF932 domain-containing protein | - |
AA953_RS23245 (AA953_23310) | 1681..2283 | - | 603 | WP_000243701 | transglycosylase SLT domain-containing protein | virB1 |
AA953_RS23250 (AA953_23315) | 2604..2987 | + | 384 | WP_001151527 | conjugal transfer relaxosome DNA-binding protein TraM | - |
AA953_RS23255 (AA953_23320) | 3174..3863 | + | 690 | WP_000283385 | conjugal transfer transcriptional regulator TraJ | - |
AA953_RS23260 (AA953_23325) | 3962..4357 | + | 396 | WP_001309237 | conjugal transfer relaxosome DNA-bindin protein TraY | - |
AA953_RS23265 (AA953_23330) | 4390..4755 | + | 366 | WP_000994779 | type IV conjugative transfer system pilin TraA | - |
AA953_RS23270 (AA953_23335) | 4770..5081 | + | 312 | WP_000012106 | type IV conjugative transfer system protein TraL | traL |
AA953_RS23275 (AA953_23340) | 5103..5669 | + | 567 | WP_000399792 | type IV conjugative transfer system protein TraE | traE |
AA953_RS23280 (AA953_23345) | 5656..6384 | + | 729 | WP_001230787 | type-F conjugative transfer system secretin TraK | traK |
AA953_RS23285 (AA953_23350) | 6384..7811 | + | 1428 | WP_000146685 | F-type conjugal transfer pilus assembly protein TraB | traB |
AA953_RS23290 (AA953_23355) | 7801..8391 | + | 591 | WP_000002792 | conjugal transfer pilus-stabilizing protein TraP | - |
AA953_RS23295 (AA953_23360) | 8378..8575 | + | 198 | WP_001443030 | conjugal transfer protein TrbD | - |
AA953_RS23300 (AA953_23365) | 8587..8838 | + | 252 | WP_001038342 | conjugal transfer protein TrbG | - |
AA953_RS23305 (AA953_23370) | 8835..9350 | + | 516 | WP_000809843 | type IV conjugative transfer system lipoprotein TraV | traV |
AA953_RS25570 (AA953_23375) | 9485..9706 | + | 222 | WP_001278689 | conjugal transfer protein TraR | - |
AA953_RS23315 (AA953_23380) | 9866..12493 | + | 2628 | WP_001064243 | type IV secretion system protein TraC | virb4 |
AA953_RS23320 (AA953_23385) | 12490..12876 | + | 387 | WP_000099690 | type-F conjugative transfer system protein TrbI | - |
AA953_RS23325 (AA953_23390) | 12873..13505 | + | 633 | WP_001203720 | type-F conjugative transfer system protein TraW | traW |
AA953_RS23330 (AA953_23395) | 13502..14494 | + | 993 | WP_000830185 | conjugal transfer pilus assembly protein TraU | traU |
AA953_RS23335 (AA953_23400) | 14503..15141 | + | 639 | WP_000777688 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
AA953_RS23340 (AA953_23405) | 15138..16946 | + | 1809 | WP_000821835 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
AA953_RS23345 (AA953_23410) | 16973..17230 | + | 258 | WP_000864318 | conjugal transfer protein TrbE | - |
AA953_RS23350 (AA953_23415) | 17223..17966 | + | 744 | WP_001030367 | type-F conjugative transfer system pilin assembly protein TraF | traF |
AA953_RS23355 (AA953_23420) | 17982..18329 | + | 348 | WP_001287913 | conjugal transfer protein TrbA | - |
AA953_RS23360 (AA953_23425) | 18331..18645 | - | 315 | WP_000415566 | protein ArtA | - |
AA953_RS23365 (AA953_23430) | 18726..19010 | + | 285 | WP_000624109 | type-F conjugative transfer system pilin chaperone TraQ | - |
AA953_RS23370 (AA953_23435) | 18997..19542 | + | 546 | WP_000059850 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
AA953_RS23375 (AA953_23440) | 19472..19813 | + | 342 | WP_001443031 | P-type conjugative transfer protein TrbJ | - |
AA953_RS23380 (AA953_23445) | 19800..20180 | + | 381 | WP_001366201 | F-type conjugal transfer protein TrbF | - |
AA953_RS23385 (AA953_23450) | 20180..21553 | + | 1374 | WP_021553026 | conjugal transfer pilus assembly protein TraH | traH |
AA953_RS23390 (AA953_23455) | 21550..24366 | + | 2817 | WP_001007055 | conjugal transfer mating-pair stabilization protein TraG | traG |
AA953_RS25575 | 24399..24920 | + | 522 | WP_000632670 | conjugal transfer entry exclusion protein TraS | - |
AA953_RS23400 (AA953_23465) | 24945..25676 | + | 732 | WP_000850422 | conjugal transfer complement resistance protein TraT | - |
AA953_RS23405 (AA953_23470) | 25929..28082 | + | 2154 | WP_000009350 | type IV conjugative transfer system coupling protein TraD | virb4 |
AA953_RS23410 (AA953_23475) | 28091..28489 | - | 399 | WP_000911324 | type II toxin-antitoxin system VapC family toxin | - |
AA953_RS23415 (AA953_23480) | 28489..28716 | - | 228 | WP_000450532 | toxin-antitoxin system antitoxin VapB | - |
Host bacterium
ID | 1172 | GenBank | NZ_CP011496 |
Plasmid name | pNCM3722-F | Incompatibility group | IncFIC |
Plasmid size | 67545 bp | Coordinate of oriT [Strand] | 2243..2532 [-] |
Host baterium | Escherichia coli strain NCM3722 isolate K-12 |
Cargo genes
Drug resistance gene | - |
Virulence gene | - |
Metal resistance gene | - |
Degradation gene | - |
Symbiosis gene | - |
Anti-CRISPR | - |