Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100711
Name   oriT_pYU39_4.8 in_silico
Organism   Salmonella enterica subsp. enterica serovar Typhimurium strain YU39 isolate YUHS 05-78
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP011433 (2412..2494 [+], 83 nt)
oriT length   83 nt
IRs (inverted repeats)      3..12, 16..25  (GTGTCGGGGC..GCCCTGACCC)
Location of nic site      56..57
Conserved sequence flanking the
  nic site    		  CTGG|CTTA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 83 nt

>oriT_pYU39_4.8
GGGTGTCGGGGCGCAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGTGGCATCAGTGCGGATT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   783 GenBank   WP_000635819
Name   Nuclease_pYU39_4.8 insolico UniProt ID   D5I152
Length   529 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 529 a.a.        Molecular weight: 59863.49 Da        Isoelectric Point: 9.8459

>WP_000635819.1 MULTISPECIES: nuclease [Bacteria]
MIVKFHARGKGGGSGPVDYLLGRERNREGATVLRGNPEEIRELIDATPFSKKYTSGVLSFAEKELPPGER
ERVMTSFERVLMPGLEKDQYSILWVEHQDKGRLELNFVIPNMELQSGKRLQPYYDRADRPRINAWQTLVN
HHYGLRDPNAPENRRTLVTPNNLPKAKQEAAEAITRGLEALYRAGAIKTRQDVTEALTAAGFEVVRTTRS
SISIADPEGGRNLRLKGALYEQSFTNGNGLREETERASRFYREHAEQRVQEARRVCRQGTELKQAENQRR
YQRTYNIRETATGYAEHHREHAAQHHRGRTVPAAEKPALAGDQRAGTVSGADRAQRHGRHFPGVLGADSV
AGKQDCEKYLDHQRAGKHAGETRGKNLGGDVRQWREREISGAAGRGERGKHLDGGRQERREAGQGVNEYD
GTGRAFAERLRAFATGLYAAAERVGERLRRIAEDARGYATEQRDTERACRELESAGAAISRTSRPLESVI
YHEQQIRDEQERQAQELARRWHEEQEPEARIWRGPSMGM

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(57-238)


  Protein structure


Source ID Structure
AlphaFold DB D5I152


Host bacterium

ID   1171 GenBank   NZ_CP011433
Plasmid name   pYU39_4.8 Incompatibility group   ColRNAI
Plasmid size   4800 bp Coordinate of oriT [Strand]   2412..2494 [+]
Host baterium   Salmonella enterica subsp. enterica serovar Typhimurium strain YU39 isolate YUHS 05-78

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -