Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100710
• Name: oriT_pC227-11-1
• Organism: Escherichia coli O104:H4 str. C227-11 isolate 368 shch
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NZ_CP011332 (17000..17449 [+], 450 nt)
• oriT length: 450 nt
• IRs (inverted repeats): IR1: 201..215, 222..236 (ATTCATTGGTGAATC..GATTCACCAATGAAT) IR2: 308..315, 318..325 (GCAAAAAC..GTTTTTGC)
• Location of nic site: 334..335
• Conserved sequence flanking the nic site: GTGGGGTGT|GG
• Note: predicted by the oriTfinder

oriT sequence

• Length: 450 nt

>oriT_pC227-11-1
ATATGTATACCTCTTTATTTTCTTATCGCATCAAATTTATTTCACATATAAAAAAATGACAATTCATCGATGAATCGAATCGTGACGCAAGTTACGAAAATTACGATTCATGCAGCAAATCACATCACGAATTGAATCTAGAGTCAATTCGCTTTAACTCGTTGTTTTATTATTACTGATTCATCTATGAGTTGCGTTAAATTCATTGGTGAATCATATGCGATTCACCAATGAATCCTTTTTATAATGTAAAATAAATTAAAATACATTATTTAAAACATAAGTTAATGATTCAAATAGCAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCTTTTTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAGGTGGAAAAATGAAAAAATGGATGTTAGCAATCT

oriT secondary structure

Predicted by RNAfold.

Relaxase

TraI_pC227-11-1

• ID: 782
• GenBank: WP_001390493
• Name: TraI_pC227-11-1
• UniProt ID: _
• Length: 1231 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 1231 a.a.
• Molecular weight: 133814.33 Da
• Isoelectric Point: 6.0682

>WP_001390493.1 conjugative transfer relaxase/helicase TraI [Escherichia coli]
MAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLQDGMTFTPGSTVIVDQGEKLSLKETLTLLDG
AARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARLAGDFAASVKAG
EESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETR
SHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKTPGLRVSGGDR
LQVASVSEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMDNATLNG
LARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETSLETAISLQKAGLHTPAQQAIHLALPV
LESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSIL
RHILEGKEAVTPLMERVPGELMETLTSGQRAATRMILEASDRFTVVQGYAGVGKTTQFRAVMSAVNMLPA
SERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAY
ALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINRDVERALSG
LESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAR
EQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVPVLNTANIRDGELRRLSTWEKNPDALALVDSVY
HRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTV
TAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYV
ALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVFEPKPDREVMNAQRLFSTARELRDVAAGRAVLRQA
GLSGGDSPARFIAPGRKYPQPYVALPAFDRNGRSAGIWLNPLTTDDGNGLRGFSGEGRVKGSEDAQFVAL
QGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIPDSSVQPGAGNGEPVT
AEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTE

Protein domains

Predicted by InterproScan.

• single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N) (121-172)

• AAA domain (AAA_30) (514-702)

• DNA helicase TraI (TraI_C) (980-1103)

• DNA relaxase TraI 2B/2B-like domain (TraI_2B) (179-258)

Protein structure

No available structure.

Host bacterium

• ID: 1170
• GenBank: NZ_CP011332
• Plasmid name: pC227-11-1
• Incompatibility group: IncFII
• Plasmid size: 75079 bp
• Coordinate of oriT [Strand]: 17000..17449 [+]
• Host baterium: Escherichia coli O104:H4 str. C227-11 isolate 368 shch

Cargo genes

• Drug resistance gene: -
• Virulence gene: aap/aspU, aggA, aggC, aggD
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -