Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100705
• Name: oriT_pSJ_3
• Organism: Escherichia coli str. Sanji
• Sequence Completeness: core
• NCBI accession of oriT (coordinates [strand]): NZ_CP011066 (39..98 [-], 60 nt)
• oriT length: 60 nt
• IRs (inverted repeats): 3..12, 16..25 (GTGTCGGGGC..GCCCTGACCC)
• Location of nic site: 57..58
• Conserved sequence flanking the nic site: CTGG|CTTA
• Note: predicted by the oriTfinder

oriT sequence

• Length: 60 nt

>oriT_pSJ_3
GGGTGTCGGGGCGCAGCCCTGACCCAGTTCACAGAGCGATAGCGATGTGTATACTGGCTT

oriT secondary structure

Predicted by RNAfold.

Relaxase

PutativerelaxaseofpSJ_3

• ID: 777
• GenBank: WP_029391997
• Name: PutativerelaxaseofpSJ_3
• UniProt ID: _
• Length: 524 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 524 a.a.
• Molecular weight: 59247.78 Da
• Isoelectric Point: 9.9130

>WP_029391997.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Bacteria]
MIVKFHARGKGGGSGPVDYLLGRERNREGATVLRGNPEEVRELIDSTPFSKKYTSGVLSFAEKELPPGER
ERVMTSFERVLMPGLEKDQYSILWVEHQDKGRLELNFVIPNMELQSGKRLQPYYDRADRPRINAWQTLVN
HHYGLRDPNAPENRRTLVTPNNLPKAKQEAAEAITRGLEALYHAGAIKTRQDVTEALTAAGFEVVRTTKS
SISIADPEGGRNLRLKGALYEQSFTNGNRLREETERASRFYREHAEQRVQEARRVCRQGIELKQAENQRR
YQRTYNIREKATGYAEHHREHAVQHHRGRTVPAAEKTALAGDQRADTVSGADRAQRHGRDFPGVLGADGV
AGKQDCEKYPDYQRAGKHAGETRGKNLGGDVRQWREREISGVAGRGERGKHLDGGRQERREAGQGVNEYD
GTGRAFAERLRAFATGLYAAAERVGERLRGIAEDARGYATEQRDTERARRDIESAGAEISRASGSLESVI
YREQQLRDEQERARYRHEVQERAARVRSGPSMEM

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (57-240)

Protein structure

No available structure.

Host bacterium

• ID: 1165
• GenBank: NZ_CP011066
• Plasmid name: pSJ_3
• Incompatibility group: _
• Plasmid size: 3373 bp
• Coordinate of oriT [Strand]: 39..98 [-]
• Host baterium: Escherichia coli str. Sanji

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -