Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100702 |
| Name | oriT_p6411-66.409kb  |
| Organism | Acinetobacter nosocomialis strain 6411 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP010903 (49102..49490 [+], 389 nt) |
| oriT length | 389 nt |
| IRs (inverted repeats) | 275..287, 292..304 (AAAAAGACCTTGC..GCAAGGTTTTTTT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 389 nt
>oriT_p6411-66.409kb
TCAAGCTGAAGCGACTGAAGAACCTGAAGTTAAAAAACTGATGGATCAATCAAACCAGAAAGAGCAGCCAGAAGCTAAAGAAGCAGATCCAGACAAAATCAAAAAACCGCTTTTAGTTGTTATTCATGACGGCCGACAAGCGAACCTGATGCTTAATAAAAAGCCTTCTTCTTTGGGTTTTGCATGGATCAAATACGAAGATGGAACAGAGGTATCTGTAGATTGCAGCAGCCTTGAAATCGAGTATTTACAAGAAGCCTAAACCTACCAATGGAAAAAGACCTTGCTTATGCAAGGTTTTTTTTGGCGGGTTATCCCGACCACCTAGCTTGATTACCCTTAGTCATGACTAAGGAGAAAAGAGGGAAAAATGAAGGGATATAATCTAG
TCAAGCTGAAGCGACTGAAGAACCTGAAGTTAAAAAACTGATGGATCAATCAAACCAGAAAGAGCAGCCAGAAGCTAAAGAAGCAGATCCAGACAAAATCAAAAAACCGCTTTTAGTTGTTATTCATGACGGCCGACAAGCGAACCTGATGCTTAATAAAAAGCCTTCTTCTTTGGGTTTTGCATGGATCAAATACGAAGATGGAACAGAGGTATCTGTAGATTGCAGCAGCCTTGAAATCGAGTATTTACAAGAAGCCTAAACCTACCAATGGAAAAAGACCTTGCTTATGCAAGGTTTTTTTTGGCGGGTTATCCCGACCACCTAGCTTGATTACCCTTAGTCATGACTAAGGAGAAAAGAGGGAAAAATGAAGGGATATAATCTAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 774 | GenBank | WP_015060290 |
| Name | MobC_p6411-66.409kb |
UniProt ID | A0A0D5LXG0 |
| Length | 222 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 222 a.a. Molecular weight: 25970.78 Da Isoelectric Point: 9.3680
>WP_015060290.1 MULTISPECIES: plasmid mobilization relaxosome protein MobC [Acinetobacter]
MTKSNLDQVLKFRVSNAVYDWWQAKCEKQGIKKGEPFQKALENAYKKENDPNYSGQDFSLNLEKDLTEKI
YQLAKFEEVDPDDLIKKMIKEKLSEIEKTQDVIDVFEKTSRKELRLKPSLMKAINEKAAQIGMKPNQYII
SVLTANVTKDSIFFSQEEALKLGESNSQLLAIGRNLNQMAKNMNQGIYESYDREFVERVHQLVKAQVRHV
FTLLERNKKRWE
MTKSNLDQVLKFRVSNAVYDWWQAKCEKQGIKKGEPFQKALENAYKKENDPNYSGQDFSLNLEKDLTEKI
YQLAKFEEVDPDDLIKKMIKEKLSEIEKTQDVIDVFEKTSRKELRLKPSLMKAINEKAAQIGMKPNQYII
SVLTANVTKDSIFFSQEEALKLGESNSQLLAIGRNLNQMAKNMNQGIYESYDREFVERVHQLVKAQVRHV
FTLLERNKKRWE
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 27773..38177
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| RR32_RS18970 (RR32_18755) | 22897..23283 | - | 387 | WP_050049536 | hypothetical protein | - |
| RR32_RS18975 (RR32_18760) | 23710..24999 | - | 1290 | WP_050049537 | LPD7 domain-containing protein | - |
| RR32_RS18980 (RR32_18765) | 25010..25291 | - | 282 | WP_050049538 | hypothetical protein | - |
| RR32_RS18985 (RR32_18770) | 25302..27776 | - | 2475 | WP_050049539 | type IV secretory system conjugative DNA transfer family protein | - |
| RR32_RS18990 (RR32_18775) | 27773..28885 | - | 1113 | WP_050049540 | P-type DNA transfer ATPase VirB11 | virB11 |
| RR32_RS18995 (RR32_18780) | 28866..30287 | - | 1422 | WP_050049541 | type IV secretion system protein VirB10 | virB10 |
| RR32_RS19000 (RR32_18785) | 30287..31177 | - | 891 | WP_050049542 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| RR32_RS19005 (RR32_18790) | 31189..32037 | - | 849 | WP_050049543 | type IV secretion system protein | virB8 |
| RR32_RS19010 (RR32_18795) | 32200..32901 | - | 702 | WP_050049544 | P-type DNA transfer protein VirB5 | virB5 |
| RR32_RS19015 (RR32_18800) | 32913..33884 | - | 972 | WP_228683080 | type IV secretion system protein | virB6 |
| RR32_RS19020 (RR32_18805) | 33962..34261 | - | 300 | WP_015060281 | hypothetical protein | - |
| RR32_RS19025 (RR32_18810) | 34264..36726 | - | 2463 | WP_050049546 | VirB4 family type IV secretion/conjugal transfer ATPase | virb4 |
| RR32_RS19030 (RR32_18815) | 36734..37060 | - | 327 | WP_005407440 | VirB3 family type IV secretion system protein | virB3 |
| RR32_RS19035 (RR32_18820) | 37060..37365 | - | 306 | WP_004644768 | TrbC/VirB2 family protein | virB2 |
| RR32_RS19040 (RR32_18825) | 37500..38177 | - | 678 | WP_050049547 | lytic transglycosylase domain-containing protein | virB1 |
| RR32_RS19045 (RR32_18830) | 38177..40429 | - | 2253 | WP_050049548 | type I DNA topoisomerase | - |
| RR32_RS19050 (RR32_18840) | 40713..40991 | - | 279 | WP_050049549 | hypothetical protein | - |
| RR32_RS19055 (RR32_18845) | 41534..41782 | + | 249 | WP_050049550 | hypothetical protein | - |
| RR32_RS19060 (RR32_18850) | 41811..42131 | - | 321 | WP_050049551 | hypothetical protein | - |
| RR32_RS19065 (RR32_18855) | 42143..42415 | - | 273 | WP_050049552 | hypothetical protein | - |
Host bacterium
| ID | 1162 | GenBank | NZ_CP010903 |
| Plasmid name | p6411-66.409kb | Incompatibility group | _ |
| Plasmid size | 66409 bp | Coordinate of oriT [Strand] | 49102..49490 [+] |
| Host baterium | Acinetobacter nosocomialis strain 6411 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |