Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100701
Name   oriT_pAcX50c in_silico
Organism   Azotobacter chroococcum NCIMB 8003
Sequence Completeness      core
NCBI accession of oriT (coordinates [strand])   NZ_CP010418 (12642..12731 [-], 90 nt)
oriT length   90 nt
IRs (inverted repeats)      1..19, 22..40  (GTGAAGATAGATAACCGGC..GCCGGTTAGCTAACTTCAC)
Location of nic site      48..49
Conserved sequence flanking the
  nic site    		  TGTCTTG|C
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 90 nt

>oriT_pAcX50c
GTGAAGATAGATAACCGGCTTGCCGGTTAGCTAACTTCACCTGTCTTGCCCGGCTCTTCGAGCCGTTTAACGCCATATAAGTATCGCATA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   773 GenBank   WP_040107071
Name   TraI_pAcX50c insolico UniProt ID   A0A0C4WL02
Length   799 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 799 a.a.        Molecular weight: 87713.68 Da        Isoelectric Point: 10.8696

>WP_040107071.1 TraI/MobA(P) family conjugative relaxase [Azotobacter chroococcum]
MISKRIPMNSAKKSSFAGLVNYITNSQGISERVGEVRITNCQSESVTWAVRDILSAQQKNTRAEGDKTYH
LLISFAPGEKPSAEVLRDVEDRICAAMGYGEHQRISAVHNDTDVLHIHVAINKIHPQRLTMHEPYRDFKT
RAEICAKLEIEHGLERVNHTGRKSLSESKANDMERHAGVESLLSWVRRECLEQIQNATSWKEMHQVLHEH
GLELRKRGNGLVIADHEGTMVKASTVARDLSKNKLEDRLGEFEGRQHGRPTPSPGTPLPKPGIGKVGRRP
PPASRNRLRSMSSLQSLKIDSGKRYEKRPVGHRTDTTELYARYKDEQSAVRHTRAKELAAERDRRDRALE
NARRAADLKRASIKLMVAPGLNKKLLYASTHKTLKEQIAKIQTEHRKAVEKINTTRKARQWADWLQVKAK
EGDQEALKALRARAGVKGLQGDAIAAKGPQKPSQAVLAGQDHITKEGTVIYLAGAAAIRDDGSKLQVSRG
TTFDGIETALRMAVARYGKQVTVTGSPQFKELVAQTAAVRNLPIKFDDPALEQRRQALQQAIEKERNNVR
ADRGRAAGTSAGGNGFDPARRGRSDTGRTTGAADGQGDGVRGSATGTGVPERLGAVDGGRIPVRALGGTA
KPGVARVGRKPPPESQNRLRALSQLGVVQLTHGAEVLLPRHVPGDVEHQGAKPTDGLRRDVSRPGVAAPT
SPISGQQAARIYIEEREEKRAKGFDIPKHRLYNQADDGAASFEGVRQVKGQFMALLKRGDDVLVLPINEA
TARRMKRMKLGDPVRATAKGAIKSKGKSR

  Protein domains


Predicted by InterproScan.

Large polyvalent protein-associated domain 7 (LPD7)

(460-548)

Relaxase/Mobilisation nuclease domain (Relaxase)

(12-251)


  Protein structure


Source ID Structure
AlphaFold DB A0A0C4WL02


T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 54014..62621

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Achr_RS20875 (Achr_c420) 49725..50747 - 1023 WP_052264040 RES family NAD+ phosphorylase -
Achr_RS24535 (Achr_c430) 51200..51814 - 615 WP_082045612 muramidase -
Achr_RS24540 (Achr_c440) 51818..52123 - 306 WP_082045613 TrbM/KikA/MpfK family conjugal transfer protein -
Achr_RS20880 (Achr_c450) 52375..53752 - 1378 Protein_46 P-type conjugative transfer protein TrbL -
Achr_RS20885 (Achr_c460) 53762..54007 - 246 WP_040107093 entry exclusion lipoprotein TrbK -
Achr_RS20890 (Achr_c470) 54014..54799 - 786 WP_040107094 P-type conjugative transfer protein TrbJ virB5
Achr_RS20895 (Achr_c480) 54823..56238 - 1416 WP_040107095 TrbI/VirB10 family protein virB10
Achr_RS20900 (Achr_c490) 56245..56697 - 453 WP_040107096 conjugal transfer protein TrbH -
Achr_RS20905 (Achr_c500) 56701..57591 - 891 WP_052264041 P-type conjugative transfer protein TrbG virB9
Achr_RS20910 (Achr_c510) 57603..58370 - 768 WP_040107097 conjugal transfer protein TrbF virB8
Achr_RS20915 (Achr_c520) 58367..60925 - 2559 WP_040107098 VirB4 family type IV secretion/conjugal transfer ATPase virb4
Achr_RS20920 (Achr_c530) 60922..61233 - 312 WP_040107099 conjugal transfer protein TrbD virB3
Achr_RS20925 (Achr_c540) 61236..61640 - 405 WP_040107100 conjugal transfer system pilin TrbC virB2
Achr_RS20930 (Achr_c550) 61659..62621 - 963 WP_040107101 P-type conjugative transfer ATPase TrbB virB11

Host bacterium

ID   1161 GenBank   NZ_CP010418
Plasmid name   pAcX50c Incompatibility group   _
Plasmid size   62783 bp Coordinate of oriT [Strand]   12642..12731 [-]
Host baterium   Azotobacter chroococcum NCIMB 8003

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -