Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100697
Name   oriT_p34977-5.006kb in_silico
Organism   Enterobacter hormaechei subsp. steigerwaltii strain 34977
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP010375 (1339..1531 [-], 193 nt)
oriT length   193 nt
IRs (inverted repeats)      22..29, 33..40  (TTCGGGGC..GCCCTGAA)
Location of nic site      73..74
Conserved sequence flanking the
  nic site    		  CTGG|CTTA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 193 nt

>oriT_p34977-5.006kb
GCGGCAGTGGTTTTAGCGGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTATTATGTTACGGCGGAGTGGTGATTTATGAAAGTGCTTCATGTAGCAAGGGAAAAATGCGGCTGCCGGGCGTAGGTGCAACATGCGGGTAAAGGTGAAATGACGCCTCCTCGCTCAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   769 GenBank   WP_040117005
Name   Putativerelaxaseofp34977-5.006kb insolico UniProt ID   A0A0E1J6W6
Length   499 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 499 a.a.        Molecular weight: 55202.40 Da        Isoelectric Point: 7.8063

>WP_040117005.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacter cloacae complex]
MIVKFHPRGRGGGAGPVDYLLGKDRQRDGASVLQGKPEEVRELIDASPYAKKYTSGVLSFAEKDLPPGQR
EKLMAGFEQVLMPGLDKDQYSVLWVEHRDKGRLELNFLIPNTELLTGRRLQPYYDRADRPRIDAWQTIVN
GRLGLHDPNAPENRRALVTPSALPEAKLEAAQAITRGLLTLAASGELKTRQDVTEALKSAGFDVVRTTKS
SISIADPDGGRNIRLKGAIYEQSFNAGEELRAEIESAAADYRRDAESRIQRAREVCQSGTERKREENQRR
HPRPRPETVLGHEPAHERHGAHGQPDVADHRPGVRAADSLRRGHSVVAGTADSRQLHDHPGADAHAGHAV
REEQRRAAHDLRGEETPLREGEPGSGTIRRGFELDDTGGEIAHDRAGKTVAERIRAATAGLLEKAGRVGE
RLRGMADDVWSYATGERGAERARHGLEQAGAEFERAAAPVVERLNGIETHREQVRAAQHQKELELERSQR
QRTYDGPSL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(57-238)


  Protein structure



No available structure.




Host bacterium

ID   1157 GenBank   NZ_CP010375
Plasmid name   p34977-5.006kb Incompatibility group   ColRNAI
Plasmid size   5007 bp Coordinate of oriT [Strand]   1339..1531 [-]
Host baterium   Enterobacter hormaechei subsp. steigerwaltii strain 34977

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -