Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100691
Name   oriT_pECC-1470_100 in_silico
Organism   Escherichia coli ECC-1470
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NZ_CP010345 (64638..65099 [-], 462 nt)
oriT length   462 nt
IRs (inverted repeats)      142..151, 154..163  (ATAGGGTCGT..ACGACCCTAT)
 231..238, 241..248  (GCAAAAAC..GTTTTTGC)
Location of nic site      257..258
Conserved sequence flanking the
  nic site    		  GTGGGGTGT|GG
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 462 nt

>oriT_pECC-1470_100
AAGAAGAGAACCCCTAGCAGCGCCCCTAGGGGCATACTATAAAAAAACGCACGGCGCTGCTAGGGGCGGCCCTAATATATCCAAATGTTTTTCATGAAAATTGTCAGCACTGACCCTAACAAGGGTCGTCATAGGGTCGCTATAGGGTCGTCAACGACCCTATTTAATAATGTAAAATAAATTAAAATACATTATCTAGAACATAAACTAATGATTTAAAAAACAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCTTTTTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAGGTGGAAAAATGAAAAAATGGATGTTAGCCATCTGCCTGATGTTTATAAATGAGATCTGCCATGCCACTGATTGCTTTGATCTTGCAGGCCGGGATTACAAAATAGATCCTGATTTACTGAGA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   763 GenBank   WP_041521561
Name   TraI_pECC-1470_100 insolico UniProt ID   _
Length   1756 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 1756 a.a.        Molecular weight: 192181.03 Da        Isoelectric Point: 6.0139

>WP_041521561.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]
MMSIAQVRSAGSAGNYYTDKDNYYVLGCMGERWAGQGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGRNRHRPGYDLTFSAPKSVSMMAMLGGDKRLIEAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDVADQRAETRTQAPGPASPDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERALAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLQEGMAFTPGNTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGRPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSNSLTLRDAQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPASLPVSDSPFMALKL
ENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETLLETAISLQKAGLHTPAQQAIHLALSVVESKNLAFSMVDLLTEAKSFAAEGTSFVDLGREINT
QIKRGDLLHVDVAKGYGTDLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPERERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEA
QQKSMLKGEAFPDVPMTLYEAIVRDYTGRTPKAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGQEQVM
VPVLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVT
LYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKQMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVL
EPKSDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGTITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGKTEQAVRDIAGLERDRSAISEREAALPESVLREPQRVREAVREVARENLLQERLQQMERDMVRDLQKE
KTLGGD

  Protein domains


Predicted by InterproScan.

TrwC relaxase (TrwC)

(10-283)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(575-626)

DNA helicase TraI (TraI_C)

(1434-1557)

AAA domain (AAA_30)

(969-1156)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(633-712)


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 64242..90442

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E1470_RS27915 (E1470_100p00700) 59646..59795 + 150 Protein_55 DUF5431 family protein -
E1470_RS24140 (E1470_100p00710) 59737..59862 + 126 WP_096937776 type I toxin-antitoxin system Hok family toxin -
E1470_RS24145 (E1470_100p00720) 60069..60851 - 783 WP_001317493 IS21-like element IS100kyp family helper ATPase IstB -
E1470_RS24150 (E1470_100p00730) 60848..61870 - 1023 WP_000255956 IS21-like element IS100 family transposase -
E1470_RS27545 (E1470_100p00740) 62123..62419 - 297 Protein_59 hypothetical protein -
E1470_RS27920 62489..62695 + 207 WP_000547963 hypothetical protein -
E1470_RS24165 (E1470_100p00750) 62720..63007 + 288 WP_000107542 hypothetical protein -
E1470_RS24170 (E1470_100p00760) 63126..63947 + 822 WP_041521559 DUF932 domain-containing protein -
E1470_RS24175 (E1470_100p00770) 64242..64844 - 603 WP_000243701 transglycosylase SLT domain-containing protein virB1
E1470_RS24180 (E1470_100p00780) 65166..65549 + 384 WP_000124981 conjugal transfer relaxosome DNA-binding protein TraM -
E1470_RS24185 (E1470_100p00790) 65739..66425 + 687 WP_000332492 PAS domain-containing protein -
E1470_RS24190 (E1470_100p00800) 66519..66746 + 228 WP_001254386 conjugal transfer relaxosome protein TraY -
E1470_RS24195 (E1470_100p00810) 66780..67145 + 366 WP_000340270 type IV conjugative transfer system pilin TraA -
E1470_RS24200 (E1470_100p00820) 67160..67471 + 312 WP_000012126 type IV conjugative transfer system protein TraL traL
E1470_RS24205 (E1470_100p00830) 67493..68059 + 567 WP_000399792 type IV conjugative transfer system protein TraE traE
E1470_RS24210 (E1470_100p00840) 68046..68774 + 729 WP_001230787 type-F conjugative transfer system secretin TraK traK
E1470_RS24215 (E1470_100p00850) 68774..70201 + 1428 WP_041521560 F-type conjugal transfer pilus assembly protein TraB traB
E1470_RS24220 (E1470_100p00860) 70191..70781 + 591 WP_000002792 conjugal transfer pilus-stabilizing protein TraP -
E1470_RS24225 (E1470_100p00870) 70768..70965 + 198 WP_001324648 conjugal transfer protein TrbD -
E1470_RS24230 (E1470_100p00880) 70977..71228 + 252 WP_001038342 conjugal transfer protein TrbG -
E1470_RS24235 (E1470_100p00890) 71225..71740 + 516 WP_000809843 type IV conjugative transfer system lipoprotein TraV traV
E1470_RS26245 (E1470_100p00900) 71875..72096 + 222 WP_001278689 conjugal transfer protein TraR -
E1470_RS24245 (E1470_100p00910) 72256..74883 + 2628 WP_001064243 type IV secretion system protein TraC virb4
E1470_RS24250 (E1470_100p00920) 74880..75266 + 387 WP_000099690 type-F conjugative transfer system protein TrbI -
E1470_RS24255 (E1470_100p00930) 75263..75895 + 633 WP_001203720 type-F conjugative transfer system protein TraW traW
E1470_RS24260 (E1470_100p00940) 75892..76884 + 993 WP_000830185 conjugal transfer pilus assembly protein TraU traU
E1470_RS24265 (E1470_100p00950) 76893..77531 + 639 WP_000777688 type-F conjugative transfer system pilin assembly protein TrbC trbC
E1470_RS24270 (E1470_100p00960) 77528..79336 + 1809 WP_000821838 type-F conjugative transfer system mating-pair stabilization protein TraN traN
E1470_RS24275 (E1470_100p00970) 79363..79620 + 258 WP_000864318 conjugal transfer protein TrbE -
E1470_RS24280 (E1470_100p00980) 79613..80356 + 744 WP_001030378 type-F conjugative transfer system pilin assembly protein TraF traF
E1470_RS24285 (E1470_100p00990) 80372..80719 + 348 WP_001287913 conjugal transfer protein TrbA -
E1470_RS24290 (E1470_100p01000) 80721..81035 - 315 WP_000415565 hypothetical protein -
E1470_RS24295 (E1470_100p01010) 81116..81400 + 285 WP_000624108 type-F conjugative transfer system pilin chaperone TraQ -
E1470_RS24300 (E1470_100p01020) 81387..81932 + 546 WP_000059834 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
E1470_RS24305 (E1470_100p01030) 81862..82203 + 342 WP_001443031 P-type conjugative transfer protein TrbJ -
E1470_RS24310 (E1470_100p01040) 82190..82582 + 393 WP_001208954 F-type conjugal transfer protein TrbF -
E1470_RS24315 (E1470_100p01050) 82569..83942 + 1374 WP_000944323 conjugal transfer pilus assembly protein TraH traH
E1470_RS24320 (E1470_100p01060) 83939..86761 + 2823 WP_001007008 conjugal transfer mating-pair stabilization protein TraG traG
E1470_RS24325 (E1470_100p01070) 86777..87274 + 498 WP_000605859 entry exclusion protein -
E1470_RS24330 (E1470_100p01080) 87306..88037 + 732 WP_000850430 conjugal transfer complement resistance protein TraT -
E1470_RS24335 (E1470_100p01090) 88289..90442 + 2154 WP_023568632 type IV conjugative transfer system coupling protein TraD virb4
E1470_RS24340 90451..90849 - 399 WP_000911317 type II toxin-antitoxin system VapC family toxin -
E1470_RS24345 90849..91076 - 228 WP_000450532 toxin-antitoxin system antitoxin VapB -

Host bacterium

ID   1151 GenBank   NZ_CP010345
Plasmid name   pECC-1470_100 Incompatibility group   IncFIB
Plasmid size   100061 bp Coordinate of oriT [Strand]   64638..65099 [-]
Host baterium   Escherichia coli ECC-1470

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -