Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100690
• Name: oriT_pAPEC-O78-3
• Organism: Escherichia coli strain 789
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NZ_CP010318 (2047..2129 [-], 83 nt)
• oriT length: 83 nt
• IRs (inverted repeats): 3..12, 16..25 (GTGTCGGGGC..GCCCTGACCC)
• Location of nic site: 56..57
• Conserved sequence flanking the nic site: CTGG|CTTA
• Note: predicted by the oriTfinder

oriT sequence

• Length: 83 nt

>oriT_pAPEC-O78-3
GGGTGTCGGGGCGCAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGTGGCATCAGTGCGGATT

oriT secondary structure

Predicted by RNAfold.

Relaxase

Nuclease_pAPEC-O78-3

• ID: 762
• GenBank: WP_000635819
• Name: Nuclease_pAPEC-O78-3
• UniProt ID: _
• Length: 529 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 529 a.a.
• Molecular weight: 59863.49 Da
• Isoelectric Point: 9.8459

>WP_000635819.1 MULTISPECIES: nuclease [Bacteria]
MIVKFHARGKGGGSGPVDYLLGRERNREGATVLRGNPEEIRELIDATPFSKKYTSGVLSFAEKELPPGER
ERVMTSFERVLMPGLEKDQYSILWVEHQDKGRLELNFVIPNMELQSGKRLQPYYDRADRPRINAWQTLVN
HHYGLRDPNAPENRRTLVTPNNLPKAKQEAAEAITRGLEALYRAGAIKTRQDVTEALTAAGFEVVRTTRS
SISIADPEGGRNLRLKGALYEQSFTNGNGLREETERASRFYREHAEQRVQEARRVCRQGTELKQAENQRR
YQRTYNIRETATGYAEHHREHAAQHHRGRTVPAAEKPALAGDQRAGTVSGADRAQRHGRHFPGVLGADSV
AGKQDCEKYLDHQRAGKHAGETRGKNLGGDVRQWREREISGAAGRGERGKHLDGGRQERREAGQGVNEYD
GTGRAFAERLRAFATGLYAAAERVGERLRRIAEDARGYATEQRDTERACRELESAGAAISRTSRPLESVI
YHEQQIRDEQERQAQELARRWHEEQEPEARIWRGPSMGM

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (57-238)

Protein structure

No available structure.

Host bacterium

• ID: 1150
• GenBank: NZ_CP010318
• Plasmid name: pAPEC-O78-3
• Incompatibility group: ColRNAI
• Plasmid size: 4599 bp
• Coordinate of oriT [Strand]: 2047..2129 [-]
• Host baterium: Escherichia coli strain 789

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -